Purification and Characterization ofγ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) fromPseudomonas paucimobilis

Nagata, Yasunobu; Hatta, Takashi; Imai, Ryozo; Kimbara, Kazuhide; Fukuda, Masao; Yano, Keiji; Takagi, Masamichi

doi:10.1271/bbb.57.1582

Cited by 65 publications

(60 citation statements)

References 5 publications

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“…LinA is a homotetrameric protein with a 16.5-kDa molecular mass (111,112), which appears to be located in the periplasm of sphingomonads (110), although a homologue in Rhodanobacter lindaniclasticus appears to be secreted extracellularly (120,173). LinA is believed to be a unique type of dehydrogenase.…”

Section: Lina Hch Dehydrochlorinase Biochemistrymentioning

confidence: 99%

“…Upon the abstraction of the proton and the formation of the double bond, the leaving-group chloride is expelled in a bimolecular elimination reaction. Only qualitative work on the substrate range of LinA has been performed so far (111), and this suggests that its substrate range may be restricted to ␣-, ␥-, and ␦-HCH and their corresponding PCCH products. Since ␤-HCH, which is not hydrolyzed by LinA, is the only major isomer that lacks at least one adjacent transdiaxial H/Cl pair ( Fig.…”

Section: Lina Hch Dehydrochlorinase Biochemistrymentioning

confidence: 99%

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Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Lal

Pandey

Sharma

et al. 2010

Microbiol Mol Biol Rev

352

338

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SUMMARY Lindane, the γ-isomer of hexachlorocyclohexane (HCH), is a potent insecticide. Purified lindane or unpurified mixtures of this and α-, β-, and δ-isomers of HCH were widely used as commercial insecticides in the last half of the 20th century. Large dumps of unused HCH isomers now constitute a major hazard because of their long residence times in soil and high nontarget toxicities. The major pathway for the aerobic degradation of HCH isomers in soil is the Lin pathway, and variants of this pathway will degrade all four of the HCH isomers although only slowly. Sequence differences in the primary LinA and LinB enzymes in the pathway play a key role in determining their ability to degrade the different isomers. LinA is a dehydrochlorinase, but little is known of its biochemistry. LinB is a hydrolytic dechlorinase that has been heterologously expressed and crystallized, and there is some understanding of the sequence-structure-function relationships underlying its substrate specificity and kinetics, although there are also some significant anomalies. The kinetics of some LinB variants are reported to be slow even for their preferred isomers. It is important to develop a better understanding of the biochemistries of the LinA and LinB variants and to use that knowledge to build better variants, because field trials of some bioremediation strategies based on the Lin pathway have yielded promising results but would not yet achieve economic levels of remediation.

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Section: Lina Hch Dehydrochlorinase Biochemistrymentioning

confidence: 99%

Section: Lina Hch Dehydrochlorinase Biochemistrymentioning

confidence: 99%

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Lal

Pandey

Sharma

et al. 2010

Microbiol Mol Biol Rev

352

338

View full text Add to dashboard Cite

show abstract

“…linA encodes dehydrochlorinase (LinA) which eliminates hydrochlorine from γ-HCH 4,5,15,20) . linA is speculated to be a gene of foreign origin due to its low GC content compared with that of other lin genes in UT26 5,15) .…”

mentioning

confidence: 99%

Presence of linA-Homologous DNA Sequences in Different Types of Soil and Their Sequence Diversity

et al. 2007

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“…It can also convert tetrachlorocyclohexadiene to 1,2,4-trichlorobenzene, but this is not the actual degradation pathway in vivo because tetrachlorocyclohexene is hydrolytically converted (see above). The enzyme is active with t~-, T-, and ~-hexachlorocyclohexane, but not with the [~-isomer or several linear chloroalkanes (42).…”

Section: Reductive Dehalogenationmentioning

confidence: 91%

Genetics and Biochemistry of Dehalogenating Enzymes

Janssen¹

1994

Annual Review of Microbiology

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Purification and Characterization ofγ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) fromPseudomonas paucimobilis

Cited by 65 publications

References 5 publications

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Presence of linA-Homologous DNA Sequences in Different Types of Soil and Their Sequence Diversity

Genetics and Biochemistry of Dehalogenating Enzymes

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