Purification and characterization of intracellular galactose oxidase from Dactylium dendroides

Directed Evolution of Galactose Oxidase: Generation of Enantioselective Secondary Alcohol Oxidases

“…GOase is a soluble, Cu‐dependent enzyme that catalyses the O 2 dependent oxidation of the C6‐OH of D ‐galactose 1 to the corresponding aldehyde 2 (Scheme ). Wild‐type GOase has a relatively narrow substrate specificity,7, 11 possessing highest activity towards specific sugars ( D ‐galactose 1 , raffinose, guar gum) and simple primary alcohols (for example, dihydroxyacetone, benzyl alcohol). A striking example of its high specificity is given by its activity towards D ‐glucose which is about 10 6 ‐fold less reactive than D ‐galactose.…”

Copper Active Sites in Biology

“…It has been reported that the mature protein contains a small amount of carbohydrate (less than 10% of the total mass), although this has not been observed in the crystal structures. 624 …”

The substrate tolerance of alcohol oxidases

“…activity for d -melibiose, up to 161 % rel. activity for d -stachyose) (Table 7 , entries 14–16) (Mendonca and Zancan 1987 ). For d -fructose (Table 7 , entry 7), a GOase mutant from Fusarium seems to be an appropriate biocatalyst (Deacon et al 2004 ).…”

“…Furthermore, galactose oxidase was active on guaran, a galactomannan (Table 7 , entry 38) (47 % rel. activity) (Mendonca and Zancan 1987 ). This enzyme was also applied for the oxidation of the nucleotide sugars uridine 5′-diphospho-α- d -galactose and uridine 5′-diphospho- N -acetyl-α- d -galactosamine (Table 7 , entry 39) for subsequent biotinylation (Bülter et al 2001 ; Namdjou et al 2007 ).…”