Purification and characterization of l-asparaginase from Pseudomonas aeruginosa strain SN004: Production optimization by statistical methods

Badoei-dalfard, Arastoo

doi:10.1016/j.bcab.2015.06.007

Cited by 39 publications

(10 citation statements)

References 50 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…L-asparagine was the ideal nitrogen source for the L-asparaginase production (170.7 Umg-1), which indicates that L-asparagine is an Lasparaginase inducer. This results in accordance with Badoei-Dalfard (2015), who confirmed that (0.5%) Lasparagine is the best source of nitrogen for P. aeruginosa strain SN004 L-asparaginase maximum production (785 U / ml). Shukla and Mandal (2013) reported that the use of L-asparagine followed by peptone and yeast extract can achieve Bacillus subtilis L-asparaginase maximum yield.…”

Section: Optimization Of P Aeruginosa Wchpa075019 Lasparaginase Productionsupporting

confidence: 91%

“…Badoei-Dalfard (2016) revealed that with Lasparaginase and glucose as nitrogen and carbon sources, the largest amount of L-asparaginase by P. pseudoalcaligens JHS-71 was obtained at pH 7.0 and 37°C after 48 h. This result was consistent with the data recorded by Badoei-Dalfard (2015), which showed that P. aeruginosa strain SN004 maximum production was achieved when glucose was used as carbon source. Various sources of organic and inorganic nitrogen have been tested.…”

Section: Optimization Of P Aeruginosa Wchpa075019 Lasparaginase Productionsupporting

confidence: 73%

See 1 more Smart Citation

roduction of Chemotherapeutic Agent L-asparaginase from Gamma-Irradiated Pseudomonas aeruginosa WCHPA075019.

2021

JJBS

View full text Add to dashboard Cite

Because of the dangers and painful effects of chemotherapeutic drugs, the need for therapeutic agents with less adverse effects will increase several times in the coming years. L-asparaginase enzyme is an effective antitumor agent, especially acute lymphoblastic leukemia, with no side effects compared to other chemotherapeutic agents. Microorganisms are emerging as a safer source of L-asparaginase. Therefore, the findings of new L-asparaginase-producing bacterial strains with high yield for therapeutic applications become necessary. From twenty bacterial isolates tested for their L-asparaginase activity, 16S rRNA sequencing for the most potent isolate showed that the selected isolate had 100% identity to pseudomonas aeruginosa strain (accession number: WCHPA075019). In the presence of L-asparagine (1%) and glucose (1%) as nitrogen and carbon sources at a low dose of gamma radiation (0.75 kGy), the maximum productivity of Lasparaginase was reached after 2 days at 35 ° C, pH 7.6 under shaking at 200 rpm. Purification of L-asparaginase with 70% ammonium sulphate, followed by Sephadex G100 increases enzyme purity by 1.5-fold after gel filtration. Pure Lasparaginase had a molecular weight of 123 kDa by SDS-PAGE. The maximum activity of the enzyme against L-asparagine was detected at 35°C and pH 9.0 after 30 min and 200 mM substrate. L-asparaginase activated in the presence of metal ions such as K+, and Na+, not affected when exposed to EDTA and strongly inhibited in the presence of Ba2+, and Cd2+. The anticancer activity of the purified enzyme was tested in vitro against three types of cell line carcinoma. The growth inhibition of L-asparaginase for HEPG2 carcinoma cell line (IC50 value of 3.5 µg/ml) was greater than the inhibition of HCT and MCF-7 carcinoma cell lines with IC50 value of 3.8 and 12.5 µg/ml, respectively relative to the growth of the untreated control cells.

show abstract

Section: Optimization Of P Aeruginosa Wchpa075019 Lasparaginase Productionsupporting

confidence: 91%

Section: Optimization Of P Aeruginosa Wchpa075019 Lasparaginase Productionsupporting

confidence: 73%

roduction of Chemotherapeutic Agent L-asparaginase from Gamma-Irradiated Pseudomonas aeruginosa WCHPA075019.

2021

JJBS

View full text Add to dashboard Cite

show abstract

“…Badoei-Dalfard purified, optimized, and characterized a bacterial L-asparaginase from a Pseudomonas aeruginosa strain. After cloning and purification, the maximum activity found using the method proposed by Imada et al [25] for the quantification of free ammonia identified 785 U/mL [26].…”

Section: Discussionmentioning

confidence: 99%

Optimization of aqueous two-phase micellar system for partial purification of L-asparaginase from Penicillium sp. grown in wheat bran as agro-industrial residue

et al. 2020

View full text Add to dashboard Cite

L-asparaginase has been used in the remission of malignant neoplasms such as acute lymphoblastic leukemia. The search for new sources of this enzyme has become attractive for therapeutics. Traditional methods for biomolecule purification involve several steps. A two-phase system may be a good strategy to anticipate one of these stages. This study aimed to produce and purify a fungal L-asparaginase through an aqueous two-phase micellar system (ATPMS) using Triton X-114. The fungus Penicillium sp.-encoded 2DSST1 was isolated from Cerrado soil. Plackett-Burman design followed by a 2 4 full factorial design was used to determine the best conditions to produce L-asparaginase. The evaluated variables were L-asparagine, L-proline, wheat bran, potato dextrose broth, ammonium sulfate, yeast extract, sucrose and glucose concentrations, incubation temperature, incubation period, and initial pH of the culture medium. L-asparaginase quantification was valued by the formation of β-aspartyl hydroxamate. The significant positive variables, L-asparagine, L-proline, potato dextrose broth, and sucrose concentrations, were evaluated at 2 levels (+ 1 and − 1) with triplicate of the central point. After 34 runs, maximum activity (2.33 IU/mL) was achieved at the factorial design central point. A central composite design was performed in ATPMS at two levels (+ 1 and − 1) varying Triton X-114 concentration (w/v), separation phase temperature, and crude extract concentration (w/v). The Lasparaginase partition coefficient (K) was considered the experimental design response. Out of the 16 systems that were examined, the most promising presented a purification factor of 1.4 and a yield of 100%.

show abstract

“…O microrganismo S. parvulus também apresentou destaque na produção de L-asparaginase quando comparada com bactérias produtoras, como a Pseudomonas aeruginosa relatada por Badoei-Dalfard (2015), que também obteve ótimos resultados, chegando a um pico de 785 U/ml. É importante ressaltar que ambos os estudos foram realizados utilizando condições semelhantes, inclusive o mesmo meio de cultura.…”

Section: Determinação Das Condições Ideais Para Produção Da Enzimaunclassified

Revitalização De Processadoras Automáticas Kodak M35 X-Omat Prox Processor

Cerutti¹,

Reis²,

Santos³

et al. 2019

Impactos Das Tecnologias Nas Ciências Biológicas E Da Saúde 2

View full text Add to dashboard Cite

show abstract

Purification and characterization of l-asparaginase from Pseudomonas aeruginosa strain SN004: Production optimization by statistical methods

Cited by 39 publications

References 50 publications

roduction of Chemotherapeutic Agent L-asparaginase from Gamma-Irradiated Pseudomonas aeruginosa WCHPA075019.

roduction of Chemotherapeutic Agent L-asparaginase from Gamma-Irradiated Pseudomonas aeruginosa WCHPA075019.

Optimization of aqueous two-phase micellar system for partial purification of L-asparaginase from Penicillium sp. grown in wheat bran as agro-industrial residue

Revitalização De Processadoras Automáticas Kodak M35 X-Omat Prox Processor

Contact Info

Product

Resources

About