Purification and Characterization of Levansucrases from<i>Bacillus amyloliquefaciens</i>in Intra- and Extracellular Forms Useful for the Synthesis of Levan and Fructooligosaccharides

Tian, Feng; Inthanavong, Lotthida; Karboune, Salwa

doi:10.1271/bbb.110315

Cited by 46 publications

(42 citation statements)

References 45 publications

(80 reference statements)

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“…Bacillus circulans (Perez Oseguera et al 1996), Bacillus amyloliquefaciens (Tian et al 2011), Bacillus sp. , and Geobacillus stearothermophilus (Inthanavong et al 2013) require sucrose as inducer to improve the levansucrase production and a very low or even no significant levansucrase production is achieved in the absence of sucrose.…”

Section: Levansucrase Production By Microbial Fermentationmentioning

confidence: 99%

“…B. amyloliquefaciens (Tian et al 2011), B. subtilis NRC33a (Abdel-Fattah et al 2005, and Bacillus sp. prefer to utilizing yeast extract to produce levansucrase, by contrast, high level of levansucrase from G. stearothermophilus is produced in the medium comprised of peptone or tryptone than yeast extract (Inthanavong et al 2013).…”

Section: Levansucrase Production By Microbial Fermentationmentioning

confidence: 99%

“…Generally, levan formation increases with an increasing concentration of sucrose (Ben Ammar et al 2002;Rairakhwada et al 2010;Tian et al 2011;Waldherr et al 2008;Zhang et al 2014), and the sucrose concentration usually plays an important role in controlling the molecular weight of the products.…”

Section: Effect Of the Sucrose Concentrationmentioning

confidence: 99%

“…B. amyloliquefaciens levansucrase synthesizes mainly levan at a very early stage of the reaction, and FOSs are only produced after the reaction proceeds for 36 h (Tian et al 2011). B. subtilis levansucrase produces levan with a DP higher than 15, as soon as the reaction is initiated.…”

Section: Production Of Levan-type Fructooligosaccharidesmentioning

confidence: 99%

“…Teixeira et al suggested that the metabolism of raffinose family oligosaccharides by lactobacilli, including raffinose, stachyose, and verbascose, involved the hydrolysis of the terminal sucrose residues by levansucrase (Teixeira et al 2012). B. amyloliquefaciens levansucrase can also use raffinose as a donor to produce levan (Tian et al 2011). In addition, Biedendieck et al reported that B. megaterium levansucrase could use some sucrose analogues, such as DGal-Fru, D-Xyl-Fru, D-Man-Fru, and D-Fuc-Fru, as novel donor substrates to produce a number of new fructosyl oligosaccharides (2007).…”

Section: Production Of Other Fructosylated Trisaccharides and Sucrosementioning

confidence: 99%

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Recent novel applications of levansucrases

Zhang

et al. 2015

Appl Microbiol Biotechnol

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Levansucrase catalyzes three distinct reactions depending on the fructosyl acceptor molecule, including polymerization, transfructosylation, and hydrolysis. As a key biocatalyst in the synthesis of levan and levan-type fructooligosaccharides, levansucrase has been widely and intensively studied. Due to the promising physiological effects of levan and levan-type fructooligosaccharides, they exhibit great potential in food and pharmaceutical industries. Another important point that attracts much attention is the wide substrate specificity of levansucrase toward monosaccharides, disaccharides, and aromatic and alkyl alcohols, producing diverse sucrose analogues, hetero-oligosaccharides (especially lactosucrose), and interesting fructosides. The present article summarizes and discusses the recent applications of microbial levansucrase in detail.

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Section: Levansucrase Production By Microbial Fermentationmentioning

confidence: 99%

Section: Levansucrase Production By Microbial Fermentationmentioning

confidence: 99%

Section: Effect Of the Sucrose Concentrationmentioning

confidence: 99%

Section: Production Of Levan-type Fructooligosaccharidesmentioning

confidence: 99%

Section: Production Of Other Fructosylated Trisaccharides and Sucrosementioning

confidence: 99%

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Recent novel applications of levansucrases

Zhang

et al. 2015

Appl Microbiol Biotechnol

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Purification and characterization of an intracellular levansucrase derived from Bacillus methylotrophicus SK 21.002

Zhang

Jiang

et al. 2015

Biotech and App Biochem

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An intracellular levansucrase from Bacillus methylotrophicus SK 21.002 was isolated, purified, and characterized. The final specific levansucrase activity was 135.40 U/mg protein with an 11.78-fold enrichment and a 9.28% recovery rate. The molecular weight of the enzyme was approximately 60,000 Da as evaluated by gel filtration and SDS-PAGE. Both the maximum transfructosylation and hydrolytic activities were observed at pH 6.5. The enzyme exhibited optimum transfructosylation activity at 40 °C, whereas the optimum temperature of hydrolytic activity was 45 °C. Cu(2+), Fe(2+), Zn(2+), and Ni(2+) inhibited both the transfructosylation and hydrolytic activities up to 100%, whereas Mn(2+) inhibited only hydrolytic activity. Ca(2+) and Mg(2+) stimulated both transfructosylation and hydrolytic activities. The chemical modifiers (n-bromosuccinimide and phenylmethanesulfonyl fluoride) strongly inhibited hydrolytic and transfructosylation activity of the levansucrase. The Km and Vmax values of the purified levansucrase were 117.2 mM and 33.23 μmol/mg·Min, respectively. When the fructose concentration was below 0.2 M, higher fructose concentrations promoted the transfructosylation and inhibited the hydrolytic activity.

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Properties of Geobacillus stearothermophilus levansucrase as potential biocatalyst for the synthesis of levan and fructooligosaccharides

Inthanavong

Tian

Khodadadi

et al. 2013

Biotechnology Progress

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The production of levansucrase (LS) by thermophilic Geobacillus stearothermophilus was investigated. LS production was more effective in the presence of sucrose (1%, w/v) than fructose, glucose, glycerol or raffinose. The results (Top 57°C; stable for 6 h at 47°C) indicate the high stability of the transfructosylation activity of G. stearothermophilus LS as compared with LSs from other microbial sources. Contrary to temperature, the pH had a significant effect on the selectivity of G. stearothermophilus LS-catalyzed reaction, favoring the transfructosylation reaction in the pH range of 6.0-6.5. The kinetic parameter study revealed that the catalytic efficiency of transfructosylation activity was higher as compared with the hydrolytic one. In addition to levan, G. stearothermophilus LS synthesized fructooligosaccharides in the presence of sucrose as the sole substrate. The results also demonstrated the wide acceptor specificity of G. stearothermophilus LS with maltose being the best fructosyl acceptor. This study is the first on the catalytic properties and the acceptor specificity of LS from G. stearothermophilus.

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Purification and Characterization of Levansucrases fromBacillus amyloliquefaciensin Intra- and Extracellular Forms Useful for the Synthesis of Levan and Fructooligosaccharides

Cited by 46 publications

References 45 publications

Recent novel applications of levansucrases

Recent novel applications of levansucrases

Purification and characterization of an intracellular levansucrase derived from Bacillus methylotrophicus SK 21.002

Properties of Geobacillus stearothermophilus levansucrase as potential biocatalyst for the synthesis of levan and fructooligosaccharides

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