Purification and Characterization of Lignin Peroxidase Isozymes from Humicola grisea (Traaen) and Its Application in Bioremediation of Textile Dyes

Abstract

PEROXIDASES have numerous important applications in industries and biodegradation of organopollutants , so there is a need to explore more and more sources of enzymes with different characteristics. In the present study, lignin peroxidases (LiPs) producing by fungus Humicola grisea were extracted and purified using gel filtration and ion exchange chromatography. Protein and heme protein were eluted in different peaks about 6 of these peaks had LiP activity and this is an indication that LiP in H. grisea was isoenzymes. Four of these isoenzymes were characterized regarding their molecular mass, optimum temperature and optimum pH for activity, substrate concentration and effect of some metal ions. The four purified isoenzymes have maximum activities at different temperatures, where optimum temperature for H2 and H4 isoenzyme were at 60 ºC, and the optimum temperatures for H1 and H3 were at 40 ºC and 30 ºC, respectively. The maximum activities of the four isoenzymes were within the acidic range. The different LiP isoenzymes showed different Km and Vmax values representing the varied affinity of the four isoenzymes using veratryl alcohol as a substrate. Km values for H1, H2, H3 and H4 were 5.26, 5.0, 1.3 and 1.4, respectively. While Vmax values for the same isoenzymes were 1.25, 0.8, 0.87 and 1.1, respectively.

In Vitro Investigation of the Impact of Bacterial–Fungal Interaction on Carbapenem-Resistant Klebsiella pneumoniae

“…The less dense zone of bacterial growth did not occur when the co-culture was allowed to grow at 26 • C. This is likely because the antibacterial agent secreted by S. brevicaulis was produced in much higher concentrations at 37 • C than at 26 • C. It is a well-established fact that fungi secrete secondary metabolites during stress conditions, and we assume that two stress factors were present in this study. The first stress factor being the high temperature at 37 • C, which is not favorable for fungal growth [41], and the second being the presence of bacteria in the same confined area [42]. To evaluate the effect of the coculture on K. pneumoniae at the molecular level, a comparison of genes responsible for antibiotic resistance, virulence factors, efflux pumps, and efflux pump related products was conducted.…”

Lignin peroxidase from the white-rot fungus Lentinus squarrosulus MPN12 and its application in the biodegradation of synthetic dyes and lignin

“…In principle, it could replace the use of chemical compounds for degrading paper waste; the reduced 5-fold concentration of lignin was observed after adding LiP enzymes in wastewater as compared with untreated wastewater, which is necessary due to the presence of lignin and its derivatives in pulp effluent increasing the pollution in the environment (Gaur et al 2018). Furthermore, LiP is also known to play a role in the decolorization of dyes in textile industrial wastewater, which was also demonstrated by Parshetti et al (2012) and Moubasher et al (2017). It is predicted that the demand for LiP will increase in the future.…”

Evaluation of Inactivation Kinetics and Thermodynamic Properties of Peroxidase from Cucumis sativus for Biotechnological and Industrial Applications

“…Peroxidase is used for Immuno labelling during research and in medical diagnosis [1] as secondary antibody. It is also useful in food processing as it serves as biological signal for the formation of harmful reactive oxygen species [7] and as catalysts for breaking down of lignocellulosic components into its fibrous units during paper pulp [8,9]. Peroxidase applications are also seen in area of recovery of polluted soils when it is being immobilize for bioremediation purposes (8).…”

“…It is also useful in food processing as it serves as biological signal for the formation of harmful reactive oxygen species [7] and as catalysts for breaking down of lignocellulosic components into its fibrous units during paper pulp [8,9]. Peroxidase applications are also seen in area of recovery of polluted soils when it is being immobilize for bioremediation purposes (8). Although peroxidases are found from various sources such as fungi, plants are the major target if it is for commercial scale applications.…”