Purification and characterization of lysozyme from filipino venus, Ruditapes philippinarum

Kim, Misook; Park, Minjeong; Jeong, Yoonhwa

doi:10.1007/s10068-012-0193-z

Cited by 12 publications

(3 citation statements)

References 28 publications

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“…LZM has an anti-inflammatory function and is the most important defense factor in fish innate immunity, directly reflecting the immunity level of zebrafish [ 28 ]. In our study, the increased activities of ACP and AKP indicated that they play an important role in the immune response, while the decrease in LZM activity may be attributable to its function as a main anti-inflammatory molecule in the response to the 6:2 FTSA stress, resulting in a large amount of consumption [ 46 ]. Similarly, in a study by Chen et al, it was found that 6:2 and 8:2 polyfluoroalkyl phosphate diesters not only accumulate and transform in carp, but also cause a significant increase in ACP and glutathione S-transferase (GST) activity in their liver tissues [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Immunotoxicity and Transcriptome Analyses of Zebrafish (Danio rerio) Embryos Exposed to 6:2 FTSA

Zhang

Ren

Chen

2023

Toxics

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As a new alternative to perfluorooctane sulfonic acid (PFOS), 6:2 fluorotelomer sulfonic acid (6:2 FTSA) has been widely produced and used in recent years, and its concentration and frequency of detection in the aquatic environment and aquatic organisms are increasing. However, studies of its toxicity in aquatic biological systems are alarmingly scarce, and the relevant toxicological information needs to be improved. In this study, we investigated AB wild-type zebrafish (Danio rerio) embryos subjected to acute 6:2 FTSA exposure for immunotoxicity using immunoassays and transcriptomics. Immune indexes showed significant decreases in SOD and LZM activities, but no significant change in NO content. Other indexes (TNOS, iNOS, ACP, AKP activities, and MDA, IL-1β, TNF-α, NF-κB, TLR4 content) all showed significant increases. These results indicated that 6:2 FTSA induced oxidative stress and inflammatory responses in zebrafish embryos and exhibited immunotoxicity. Consistently, transcriptomics showed that genes involved in the MAPK, TLR and NOD-like receptor signaling pathways (hsp70, hsp701, stat1b, irf3, cxcl8b, map3k8, il1b, tnfa and nfkb) were significantly upregulated after 6:2 FTSA exposure, suggesting that 6:2 FTSA might induce immunotoxicity in zebrafish embryos through the TLR/NOD-MAPK pathway. The results of this study indicate that the safety of 6:2 FTSA should be examined further.

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Section: Discussionmentioning

confidence: 99%

Immunotoxicity and Transcriptome Analyses of Zebrafish (Danio rerio) Embryos Exposed to 6:2 FTSA

Zhang

Ren

Chen

2023

Toxics

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“…Researches indicate the presence of yet another hydrolytic enzyme in the plant system called lysozyme (EC 3.2.1.17), which has been purified, and their properties have been studied in a few plant species such as Carica papaya, Ficus glabrata, Rubus hispidus, Parthenocissus quinquifolia, Asclepias syriaca, and Triticum vulgare [3][4][5][6][7][8]. Recently, lysozymes from other animal sources such as snails of marine origin namely Cellana nigrolineata [9] and Ruditapes philippinarum [10] and from Emu (Dromaius novaehollandiae) egg white [11] have been purified and characterized.…”

Section: Introductionmentioning

confidence: 99%

A Single-Step Purification of Cauliflower Lysozyme and Its Dual Role Against Bacterial and Fungal Plant Pathogens

Muthu

Balasubramaniam

Chun

2015

Appl Biochem Biotechnol

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A novel lysozyme from cauliflower was purified in a single step, for the first time, using Sephadex G100 column chromatography. The purified lysozyme exhibited a homogenized single band in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and its molecular mass was calculated to be 22.0 kDa. The purified lysozyme showed activity between 30 to 60 °C with 40 °C as the optimum temperature for its maximal activity. Although the purified lysozyme was functional at pH ranges between 3.0 and 9.0, the optimum pH for the enzyme activity was 8.0. By Michaelis-Menten equation, the threshold substrate concentration for the optimal enzyme activity was calculated to be 133.0 μg. The purified lysozyme showed extraordinary activity against plant pathogenic bacteria and fungi. At 10-μg concentrations, it inhibited the growth of plant pathogenic bacteria such as Pseudomonas syringae, Xanthomonas campestris, and Erwinia carotovora exhibiting 4.28, 5.90, and 3.88-fold inhibition, respectively. Further, it also completely inhibited the conidial germination of Archemonium obclavatum and, to a very large extent, other fungal species such as Fusarium solani (79.3 %), Leptosphaeria maculans (88.6 %), Botrytis cinera (73.3 %), Curvularia lunata (68 %), Rhizoctonia solani (79.6 %), and Alternaria alternata (83.6 %).

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“…It also has many applications in the current biopharmaceutical industry, including medicinal use, antibacterial, hemostasis, the reduction of swelling and inflammation and an important soluble agent for cell-free extracts in the fermentation industry. Chicken egg white contains the highest amount of lysozyme, making it the best source of purified lysozyme (Chiu et al, 2012;Kim et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Effective and Low-cost Purification of Lysozyme by Combination of Conventional Processes

Ma¹,

Xue²,

Li³

et al. 2014

AJFST

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Purification and characterization of lysozyme from filipino venus, Ruditapes philippinarum

Cited by 12 publications

References 28 publications

Immunotoxicity and Transcriptome Analyses of Zebrafish (Danio rerio) Embryos Exposed to 6:2 FTSA

Immunotoxicity and Transcriptome Analyses of Zebrafish (Danio rerio) Embryos Exposed to 6:2 FTSA

A Single-Step Purification of Cauliflower Lysozyme and Its Dual Role Against Bacterial and Fungal Plant Pathogens

Effective and Low-cost Purification of Lysozyme by Combination of Conventional Processes

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