Purification and characterization of peroxidases from Withania somnifera (AGB 002) and their ability to oxidize IAA

Johri, Sarojini; Jamwal, Urmila; Rasool, Shafaq; Kumar, Arvind; Verma, Ved Vrat; Qazi, Ghulam N.

doi:10.1016/j.plantsci.2005.05.015

Cited by 45 publications

(32 citation statements)

References 31 publications

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“…4b), where the molecular weight of POII had the same value. This value is similar to the molecular weight value of buck wheat peroxidase (56,100 kDa) [23] and higher than those reported for peroxidases from papaya fruit (41-54 kDa) [24], oranges (22 to 44 kDa) [8], kiwi fruit (40-42 kDa) [25], pea nuts (40-42 kDa) [26], horse radish (44 kDa) [27] and Withania somnifera (34-48 kDa) [28]. The highest molecular weight was detected for marula fruit peroxidase (71 kDa) [29].…”

Section: Resultssupporting

confidence: 84%

“…POII had no peroxidase activity toward the other examined substrates especially ascorbic acid indicating that this enzyme does not belong to the family of ascorbate peroxidases. Similarly, W. somnifera peroxidases readily catalyzed the oxidation of phynolic substrates like guaiacol and o-dianisidine and did not catalyze ascorbic acid [28]. However, Cucumis sativus peroxidases catalyzed the oxidation of pyrogallol and guaiacol than that of ascorbate [31].…”

Section: Resultsmentioning

confidence: 99%

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Properties of a Cationic Peroxidase from Citrus jambhiri cv. Adalia

Mohamed

El-Badry

Drees

et al. 2008

Appl Biochem Biotechnol

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The major pool of peroxidase activity is present in the peel of some Egyptian citrus species and cultivars compared to the juice and pulp. Citrus jambhiri cv. Adalia had the highest peroxidase activity among the examined species. Four anionic and one cationic peroxidase isoenzymes from C. jambhiri were detected using the purification procedure including ammonium sulfate precipitation, chromatography on diethylaminoethanol-cellulose, carboxymethyl-cellulose, and Sephacryl S-200 columns. Cationic peroxidase POII is proved to be pure, and its molecular weight was 56 kDa. A study of substrate specificity identified the physiological role of POII, which catalyzed the oxidation of some phenolic substrates in the order of o-phenylenediamine > guaiacol > o-dianisidine > pyrogallol > catechol. The kinetic parameters (K (m), V (max), and V (max)/K (m)) of POII for hydrolysis toward H2O2 and electron donor substrates were studied. The enzyme had pH and temperature optima at 5.5 and 40 degrees C, respectively. POII was stable at 10-40 degrees C and unstable above 50 degrees C. The thermal inactivation profile of POII is biphasic and characterized by a rapid decline in activity on exposure to heat. The most of POII activity (70-80%) was lost at 50, 60, and 70 degrees C after 15, 10, and 5 min of incubation, respectively. Most of the examined metal ions had a very slight effect on POII except of Li+, Zn2+, and Hg2+, which had partial inhibitory effects. In the present study, the instability of peroxidase above 50 degrees C makes the high temperature short time treatment very efficient for the inactivation of peel peroxidase contaminated in orange juice to avoid the formation of off-flavors.

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Properties of a Cationic Peroxidase from Citrus jambhiri cv. Adalia

Mohamed

El-Badry

Drees

et al. 2008

Appl Biochem Biotechnol

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“…Similarly, Ahn et al (2002) while studying the activity profile of POX during tomato fruit ripening reported two major isozymes whereas Ullrich et al (2004) observed a total of 6 isozymes in Agrocybe aegerita (a mushroom). Number of isoforms have been reported to be four in roots of 5 month old Withania somnifera (AGB002) (Johri et al 2005) and in the skin of maturing tomato fruit (Andrews et al 2000). Bernardi et al (2004), in two poplar clones differing in sensitivity towards ozone exposure, found six POX isozymes in young leaves and seven in mature leaves, indicating expression of one new POX during maturity.…”

Section: Resultsmentioning

confidence: 99%

Isozymes of antioxidative enzymes during ripening and storage of ber (Ziziphus mauritiana Lamk.)

et al. 2011

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Isozyme profile of antioxidative enzymes viz. superoxide dismutase (SOD), peroxidase (POX), catalase (CAT) and ascorbate peroxidase (APX) was studied during ripening and storage of two cultivars of ber fruit (Ziziphus mauritiana Lamk.) differing in their shelf-lives viz. Umran (shelf-life, 8-9 d) and Kaithali (shelf-life, 4-5 d). The profile revealed that Umran variety exhibited three bands each of SOD and POX while in Kaithali, these enzymes had two isoenzymes throughout ripening. CAT and APX, however, showed two isozymes each during ripening of both the varieties and the pattern remained the same at all the stages of ripening except at the initial stage i.e immature green stage where single CAT isozyme was visible. During storage, one extra band each of SOD and POX present only in Umran got disappeared at later stages of storage, whereas in Kaithali, the pattern remained unchanged. Also, there was no change in the pattern of CAT and APX isozymes during storage of both the varieties. One isozyme of CAT could be considered as ripening related while one isozyme each of SOD and POX could be related to higher shelf life of fruits.

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“…Another study has detected nicotine, somniferine, somniferinine, withanine, withananine, pseudowithanine, tropine, pseudotropine, 3a-tigloyloxytropane, choline, cuscohygrine, dl-isopelletierine and new alkaloids anaferine and anhygrine in this medicinal plant (Gupta and Rana 2007). The reported total alkaloid content in the roots of Indian W. somnifera varies between 0.13 and 0.31%, though much higher yields (up to 4.3%) have been recorded in plants of other regions/countries (Johri et al 2005). Apart from these contents the plant also contains chemical constituents like acylsteryl glucosides, starch, hantreacotane, ducitol, and a variety of amino acids including aspartic acid, proline, tyrosine, alanine, glycine, glutamic acid, cystine, tryptophan, and high amount of iron (Gupta and Rana 2007).…”

Section: Some Other Components In W Somniferamentioning

confidence: 99%

Recent advances in elucidating the biological properties of Withania somnifera and its potential role in health benefits

et al. 2011

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Withania somnifera (L.) Dunal (Solanaceae), also known as ashwagandha, is an important medicinal plant that is widely used as a home remedy for several diseases in the Indian subcontinent and other parts of the world. W. somnifera is a dietary supplement composed of various nutrients, polyphenols and alkaloids that have free radical scavenging capacity, as well as other chemical constituents that possess anti-inflammatory, antitumor, anti-stress, antioxidant, immunomodulatory, and rejuvenating properties. The mechanism of action for these properties are not fully understood. W. somnifera also appears to influence the endocrine, cardiopulmonary and central nervous systems. Toxicity studies reveal that W. somnifera can be used without side effects. The findings presented in this review are very encouraging and indicate that this herb should be studied more extensively to confirm these results and to reveal other potential therapeutic effects.

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Purification and characterization of peroxidases from Withania somnifera (AGB 002) and their ability to oxidize IAA

Cited by 45 publications

References 31 publications

Properties of a Cationic Peroxidase from Citrus jambhiri cv. Adalia

Properties of a Cationic Peroxidase from Citrus jambhiri cv. Adalia

Isozymes of antioxidative enzymes during ripening and storage of ber (Ziziphus mauritiana Lamk.)

Recent advances in elucidating the biological properties of Withania somnifera and its potential role in health benefits

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