Purification and characterization of phycocyanin from the marine cyanobacterium Synechococcus sp. IO9201

Abalde, Julio; Betancourt, L. M.; Torres, Enrique; Cid, Ángeles; Barwell, C. J.

doi:10.1016/s0168-9452(98)00113-7

Cited by 170 publications

(107 citation statements)

References 22 publications

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“…C-phycocyanin purity (EP): The C-phycocyanin purity was calculated spectrophotometrically using Equation 2 (Abalde et al 1998). Where: OD 620 is the optical density of the sample at 620 nm and OD 280 is the optical density of the sample at 280 nm.…”

Section: Experimental Designsmentioning

confidence: 99%

Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design

Antelo

Costa

Kalil

2015

Braz. arch. biol. technol.

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C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the conditions of the purification was studied using a central composite rotatable design for each molar mass of PEG. The ATPS composed of 7% (w/w) PEG

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Section: Experimental Designsmentioning

confidence: 99%

Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design

Antelo

Costa

Kalil

2015

Braz. arch. biol. technol.

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“…On the other hand, when the pH is below its pI the biomolecule will be positively charged. The isoelectric point of C-phycocyanin is around 4.6 to 5.2 (SantiagoSantos et al, 2004;Abalde et al, 1998), and thus the extract used in this study was negatively charged and the adsorbents were anionic. Hence, when C-phycocyanin made contact with the adsorbent, the bufferadsorbent bond formed was weaker than the C-phycocyanin-adsorbent bond, resulting in an adsorption process.…”

Section: Effects Of Ph On the Partition Coefficientmentioning

confidence: 96%

Kinetics and adsorption isotherm of C-phycocyanin from Spirulina platensis on ion-exchange resins

Sala

Figueira

Cerveira

et al. 2014

Braz. J. Chem. Eng.

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-C-phycocyanin is a natural blue dye extracted from Spirulina platensis, which has many applications in the food and pharmaceutical industries. In this paper the effect of pH and temperature on the adsorption of C-phycocyanin onto two different ion exchange resins (Streamline DEAE and Streamline Q XL) for expanded bed adsorption chromatography was investigated. Moreover, the kinetics and adsorption isotherm were evaluated. The equilibrium for the Q XL matrix was reached after 60 min, while for DEAE it was only reached after 140 min. C-phycocyanin showed the highest partition coefficient at pH 7.5 for both resins at 25 °C. The C-phycocyanin adsorption isotherm was very well represented by the Langmuir, Freundlich and Langmuir-Freundlich models, where the estimated values for Q m and K d obtained by the Langmuir isotherm were, respectively, 33.92 mg.mL -1 and 0.123 mg.mL -1 for DEAE, and 28.12 mg.mL -1 and 0.082 mg.mL -1 for the Q XL matrix. A negative cooperativity was observed for C-phycocyanin binding when the Q XL matrix was used, while the cooperativity was purely independent using the DEAE matrix.

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“…PBPs are also used in many other food products such as fermented milk products, ice creams, soft drinks, desserts, sweet cake decoration, milk shakes, maintaining the color for at least 1 mo at room temperature. Experiments of [71] Rhodella violace Gradient centrifugation; Hydroxylapatite chromatography; Preparative "native" PAGE --PC Abalde et al [72] Synechochoccus sp. Hydrophobic interaction chromatography; Ion exchange chromatography 4.85 -PC Zhang et al [41] S. platensis Ammonum sulphate fractionation; Ion exchange chromatography; Gel filtration chromatography 5.06 -PE Galland-Irmouli et al [73] Palmaria palmate Preparative "native" PAGE 3.2 -PC Rito-Palomares et al [74] S. maxima Two-phase aqueous extraction; Ultrafiltration; Ammonum sulphate precipitation 3.8 29.5 PC Minkova et al [75] S. fusiformis Rivanol treatment; Ammonum sulphate precipitation; Gel filtration chromatography; Ammonum sulphate precipitation 4.3 45.7 PC Soni et al [39] O. quadripunctulata Ammonum sulphate precipitation; Gel filtration chromatography; Anion exchange chromatography 3.…”

Section: Colorant In Foodsmentioning

confidence: 99%

Recent advances in production, purification and applications of phycobiliproteins

Sonani

Rastogi

Patel

et al. 2016

WJBC

159

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light-harvesting, PBPs are found to involve in several life sustaining phenomena including photoprotection in cyanobacteria. The unique spectral features (like strong absorbance and fluorescence), proteineous nature and, some imperative properties like hepato-protective, antioxidants, anti-inflammatory and anti-aging activity of PBPs enable their use in food, cosmetics, pharmaceutical and biomedical industries. PBPs have been also noted to show beneficial effect in therapeutics of some disease like Alzheimer and cancer. Such large range of applications increases the demand of PBPs in commodity market. Therefore, the large-scale and coast effective production of PBPs is the real need of time. To fulfil this need, many researchers have been working to find the potential producer of PBPs for the production and purification of PBPs. Results of these efforts have caused the inventions of some novel techniques like mixotrophic and heterotrophic strategies for production and aqueous two phase separation for purification purpose. Overall, the present review summarises the recent findings and identifies gaps in the field of production, purification and applications of this biological and economically important proteins. Core tip: The present review compiles the overall research that has been done so far in the field of produ ction, purification and application of phycobiliptoteins (PBPs). The comparative accounts for different methods for PBPs production, purification have been discussed. The potential applications of PBPs in various industries including food, cosmetics and pharmaceutical have been debated rationally. Remaining questions and rese arch gaps in respected field have been highlighted as conclusion. Recent advances in production, purification and applications of phycobiliproteins AbstractAn obligatory sunlight requirement for photosynthesis has exposed cyanobacteria to different quantity and quality of light. Cyanobacteria can exhibit efficient photosynthesis over broad region (450 to 650 nm) of solar spectrum with the help of brilliantly coloured pigment proteins called phycobiliproteins (PBPs). Besides REVIEW 100February 26, 2016|Volume 7|Issue 1|

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Purification and characterization of phycocyanin from the marine cyanobacterium Synechococcus sp. IO9201

Cited by 170 publications

References 22 publications

Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design

Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design

Kinetics and adsorption isotherm of C-phycocyanin from Spirulina platensis on ion-exchange resins

Recent advances in production, purification and applications of phycobiliproteins

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