Purification and characterization of plantaricin Y, a novel bacteriocin produced by Lactobacillus plantarum 510

Abstract

Lactobacillus plantarum 510, previously isolated from a koshu vineyard in Japan, was found to produce a bacteriocin-like inhibitory substance which was purified and characterized. Mass spectrometry analysis showed that the mass of this bacteriocin is 4,296.65 Da. A partial sequence, NH2- SSSLLNTAWRKFG, was obtained by N-terminal amino acid sequence analysis. A BLAST search revealed that this is a unique sequence; this peptide is thus a novel bacteriocin produced by Lactobacillus plantarum 510 and was termed plantaricin Y. Plantaricin Y shows strong inhibitory activity against Listeria monocytogenes BCRC 14845, but no activity against other pathogens tested. Bacteriocin activity decreased slightly after autoclaving (121 °C for 15 min), but was completely inactivated by protease K. Furthermore, trypsin-digested bacteriocin product fragments retained activity against L. monocytogenes BCRC 14845 and exhibited a different inhibitory spectrum.

Metabolic role of lactobacilli in weight modification in humans and animals

“…The production of bacteriocins has been described for several strains of lactobacilli in 350 weight-gain and weight-loss associated genomes [126][127][128][129][130][131][132]. However, weight gain-associated 351 genomes were found to encode more bacteriocins than weight loss-associated genomes [88].…”

Purification and Molecular Characterization of Two-Antimicrobial Peptides Produced by Lactobacillus plantarum DU10

“…Plantaricin LD1 produced by food isolate of Lactobacillus plantarum LD1 inhibited not only related strains but also other gram-positive and gram-negative bacteria such as S. aureus, P. aeruginosa, S. typhi as described by Gupta and Tiwari (2014). Plantaricin Y produced by L. plantarum 510 reported by Chen et al (2014) showed strong inhibitory activity against L. monocytogenes BCRC 14845.…”

“…They are completely active usually because of the synergistic action of the two peptides, while each peptide alone has less or no activity. In all previous studies, the optimal combination ratio of the two peptides is about 1:1 (Chen et al, 2014).…”

Detection and Characterization of Weissellicin 110, a Bacteriocin Produced by Weissella cibaria

“…The harvested cell-free supernatant was desalted and purified using a C 18 cartridge (Sep-Pak C 18 ; Waters, Milford, MA, USA) following Chen et al (17). Antimicrobial activity of the peptide fractions was tested using agar-spot diffusion assay (18).…”