Purification and characterization of potato lectin.

Matsumoto, Isamu; Jimbo, Akiko; Mizuno, Yasuko; Seno, Nobuko; Jeanloz, Roger W.

doi:10.1016/s0021-9258(18)32801-1

Cited by 45 publications

(3 citation statements)

References 55 publications

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“…Concanavalin ensiformis (ConA) [ 34 ] were obtained from Dako (Carpinteria, CA, USA). The lectin obtained from Vector Laboratories (Burlingame, CA, USA) was Solanum tuberosum (STA) [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

“… Mesopolysaccharide (MPS) of murine small bowel MPS. The glucose/mannose group lectin Concanavalin ensiformis (ConA) [ 34 ] ( A ) and the N-acetylglucosamine group lectin Solanum tuberosum (STA) [ 35 ] ( B ) were used to demonstrate carbohydrates on the serosal surface (white arrows; bar = 50 µm). ( C ) Transmission electron microscopy (TEM) of the serosa demonstrating mesothelial cells (mc) and microvilli (mv).…”

Section: Figurementioning

confidence: 99%

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Biomaterial-Assisted Anastomotic Healing: Serosal Adhesion of Pectin Films

et al. 2021

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Anastomotic leakage is a frequent complication of intestinal surgery and a major source of surgical morbidity. The timing of anastomotic failures suggests that leaks are the result of inadequate mechanical support during the vulnerable phase of wound healing. To identify a biomaterial with physical and mechanical properties appropriate for assisted anastomotic healing, we studied the adhesive properties of the plant-derived structural heteropolysaccharide called pectin. Specifically, we examined high methoxyl citrus pectin films at water contents between 17–24% for their adhesivity to ex vivo porcine small bowel serosa. In assays of tensile adhesion strength, pectin demonstrated significantly greater adhesivity to the serosa than either nanocellulose fiber (NCF) films or pressure sensitive adhesives (PSA) (p < 0.001). Similarly, in assays of shear resistance, pectin demonstrated significantly greater adhesivity to the serosa than either NCF films or PSA (p < 0.001). Finally, the pectin films were capable of effectively sealing linear enterotomies in a bowel simulacrum as well as an ex vivo bowel segment. We conclude that pectin is a biomaterial with physical and adhesive properties capable of facilitating anastomotic healing after intestinal surgery.

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Section: Methodsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Biomaterial-Assisted Anastomotic Healing: Serosal Adhesion of Pectin Films

et al. 2021

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“…(D. stramonium plants were grown in an University of Edinburgh Department of Botany greenhouse.) The lectin from potato fruits was prepared as previously described (Kilpatrick, 1980), and that from potato tubers in the same way after initial extraction of grated tubers in 3M-acetic acid and precipitation by (NH4)2SO4 at 55% saturation (Matsumoto et al, 1983). The potato-fruit and -tuber lectins formed a line of identity when placed in adjacent wells and subjected to immunodiffusion against antisera raised against the tuber lectin.…”

Section: Lectinsmentioning

confidence: 99%

A comparison of tomato (Lycopersicon esculentum) lectin with its deglycosylated derivative

Kilpatrick¹,

Graham²,

Urbaniak³

et al. 1984

Biochemical Journal

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A deglycosylated derivative of tomato (Lycopersicon esculentum) lectin was prepared with the use of trifluoromethanesulphonic acid. Its properties were generally similar to those of the native lectin, but differences were evident in terms of relative agglutinating activity towards sheep, (untreated) human and trypsin-treated human erythrocytes. The two forms of tomato lectin were used in conjunction with a battery of specific antisera to investigate structural relatedness among solanaceous lectins. Immunological cross-reactivity between tomato, potato and Datura lectins depends on the integrity of the glycosylated region of those lectins; that between Datura lectin and other seed lectins, however, has a separate structural basis.

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Structure and Function of Plant Lectins

Rüdiger¹

1996

Glycosciences

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Purification and characterization of potato lectin.

Cited by 45 publications

References 55 publications

Biomaterial-Assisted Anastomotic Healing: Serosal Adhesion of Pectin Films

Biomaterial-Assisted Anastomotic Healing: Serosal Adhesion of Pectin Films

A comparison of tomato (Lycopersicon esculentum) lectin with its deglycosylated derivative

Structure and Function of Plant Lectins

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