Purification and Characterization of the [NiFe]-Hydrogenase of Shewanella oneidensis MR-1

Shi, Liang; Belchik, Sara M.; Plymale, Andrew E.; Heald, Steve M.; Dohnálková, Alice; Sybirna, Kateryna; Bottin, Hervé; Squier, Thomas C.; Zachara, John M.; Fredrickson, James K.

doi:10.1128/aem.00260-11

Cited by 33 publications

(35 citation statements)

References 44 publications

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“…This dual reduction pattern at the cell surface and in the inner compartments was observed for U(VI) and Cr(VI) (84,94). Both compounds alter their solubility upon reduction.…”

Section: Extracellular Electron Acceptorsmentioning

confidence: 82%

Dissimilatory Reduction of Extracellular Electron Acceptors in Anaerobic Respiration

Richter

Schicklberger

Gescher

2012

Appl Environ Microbiol

246

181

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An extension of the respiratory chain to the cell surface is necessary to reduce extracellular electron acceptors like ferric iron or manganese oxides. In the past few years, more and more compounds were revealed to be reduced at the surface of the outer membrane of Gram-negative bacteria, and the list does not seem to have an end so far. Shewanella as well as Geobacter strains are model organisms to discover the biochemistry that enables the dissimilatory reduction of extracellular electron acceptors. In both cases, c-type cytochromes are essential electron-transferring proteins. They make the journey of respiratory electrons from the cytoplasmic membrane through periplasm and over the outer membrane possible. Outer membrane cytochromes have the ability to catalyze the last step of the respiratory chains. Still, recent discoveries provided evidence that they are accompanied by further factors that allow or at least facilitate extracellular reduction. This review gives a condensed overview of our current knowledge of extracellular respiration, highlights recent discoveries, and discusses critically the influence of different strategies for terminal electron transfer reactions.

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“…This dual reduction pattern at the cell surface and in the inner compartments was observed for U(VI) and Cr(VI) (84,94). Both compounds alter their solubility upon reduction.…”

Section: Extracellular Electron Acceptorsmentioning

confidence: 82%

Dissimilatory Reduction of Extracellular Electron Acceptors in Anaerobic Respiration

Richter

Schicklberger

Gescher

2012

Appl Environ Microbiol

246

181

View full text Add to dashboard Cite

show abstract

“…In addition, both redox processes should contribute to the bioeletrocatalytic electron transfer and they might be related to outer membrane cytochromes of exoelectrogenic G. sulfurreducens, which are crucial for the electron transfer from electricigens to a solid anode acceptor, such as OmcB, OmcE and OmcS, as reported previously. 45,46 Taking OmcB as an example, a typical potential of À0.19 V vs. SHE was reported, 47 and such potential appeared most likely to represent the redox system E f,1 (À0.374 V vs. SCE) from this study. The presence of a second involved species at a more positive potential was also reported in prior studies, indicating a parallel and similar membrane-associated species.…”

mentioning

confidence: 55%

Macroporous monolithic Magnéli-phase titanium suboxides as anode material for effective bioelectricity generation in microbial fuel cells

You

Liu

et al. 2016

J. Mater. Chem. A

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A macroporous monolithic ceramic anode material based on Magnéli-phase titanium suboxides, fabricated by a facile method, is able to effectively generate bioelectricity in microbial fuel cells (MFCs). Owing to their highly active surface area and efficient extracellular electron transfer from electricigens to the anode, MFCs can achieve a peak biocurrent time of 37 h with a maximum power density of 1541 AE 18 mW m À2 .Microbial fuel cells (MFCs) are able to convert chemical energy from organic substances into electricity by an exoelectrogenic biolm grown on the anode, which offers a sustainable technology to recover energy from organic waste. 1 In MFCs, the electrons stored in organic matter are rst released during the microbial metabolism, followed by transfer to the anode through the extracellular electron transfer (EET) process. The EET can proceed via direct contact of c-type cytochromes on the bacterial outer membrane, 2 long-distance transport by conductive bacterial pili (i.e. nanowires), 3 and indirect mediated transfer via electron shuttles.4 As EET constitutes one of the most signicant steps that limit the magnitude of power produced in an MFC, there is an increasing interest in developing effective anode materials and structures for electroactive biolm attachment and power generation.Much effort has been invested in exploring anode materials for the enrichment of exoelectrogenic bacteria. These anode materials should have an open-cell macro-and mesoscale porous structure to allow a multi-dimensional colonization and to increase the anolyte-biolm-anode interfacial area for mass transfer and electron transport.5 For materials containing 2D and 3D microscale pores, the rapid microbial growth will clog the pores and hinder the inner colonization and substrate diffusion.6 Moreover, the long-term mechanical strength and stability of conventional carbon-based electrodes may be another concern as a consequence of interfacial corrosion and oxidation of the anode material.7 Based on these considerations, it will be highly desirable to develop macroporous, highly conductive and chemically stable electrode materials for efficient attachment of electrochemically active microorganisms and viable electricity generation in MFC systems.Titanium dioxide (TiO 2 ) is a well-known semi-conductor, which has been widely used in photocatalytic reactions. Interestingly, the electronic properties of TiO 2 can be drastically transformed by establishing oxygen deciencies within the crystalline lattice, affording what is known as Magnéli-phase titanium suboxides, with a generic formula of Ti x O 2xÀ1 (4 # x # 10).8 Magnéli-phase titanium suboxides have a crystal structure with an oxygen deciency for every xth layer, resulting in shear planes where 2D chains of octahedra become face-sharing to accommodate the deciency in oxygen. This unique structure leads to a combination of outstanding electrical conductivity similar to that of metals and a great corrosion resistance close to that of ceramic materials.9 For example, Ti 4...

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“…An understanding of the hydrogenase enzyme system, which functions to catalytically mediate proton-coupled electron transfer mechanisms to form hydrogen and has been shown to reduce Tc(VII) 74 , also is relevant to bioremediation. The availability of high-resolution crystal structures of hydrogenase enzymes stabilized in specific intermediate states will enable analysis of how function is conferred by conformational changes.…”

Section: Utem Permits Measurements Of Assembly and Functional Dynamicmentioning

confidence: 99%

“…Evidence suggests the aggregation behavior of engineered nanoparticles roughly follows the classical behavior of colloidal particles in aquatic systems. 74 Therefore, the key challenge is to determine the deposition behavior of nanoparticles to understand transport at reduced length scales. The need to characterize and understand nanoparticle deposition behavior in complex matrices and environments 102 echoes the dilemma faced in characterizing nanoparticles.…”

Section: Transport Of Nanoparticlesmentioning

confidence: 99%

Dynamic Processes in Biology, Chemistry, and Materials Science: Opportunities for UltraFast Transmission Electron Microscopy - Workshop Summary Report

Kabius¹,

Browning²,

Thevuthasan³

et al. 2012

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This report summarizes a 2011 workshop held at the Environmental Molecular Sciences Laboratory (EMSL) in Richland, Washington, that addressed the potential role of rapid, time-resolved electron microscopy measurements in accelerating the solution of important scientific and technical problems. A series of U.S. Department of Energy (DOE) and National Academy of Science workshops have highlighted the critical role advanced research tools play in addressing scientific challenges relevant to biology, sustainable energy, and technologies that will fuel economic development without degrading our environment. Among the specific capability needs for advancing science and technology are tools that extract more detailed information in realistic environments (in situ or operando) at extreme conditions (pressure and temperature) and as a function of time (dynamic and time-dependent). One of the DOE workshops, "Future Science Needs and Opportunities for Electron Scattering: Next Generation Instrumentation and Beyond," specifically addressed the importance of electron-based characterization methods for a wide range of energy-relevant Grand Scientific Challenges. Boosted by the electron optical advancement in the last decade, a diversity of in situ capabilities already is available in many laboratories. These capabilities enable the investigations of biological and chemical processes in situ with the high spatial and spectroscopic resolution provided by transmission electron microscopy (TEM). The remaining major capability gap is the lack of time resolution. State-of-the-art in situ TEM instrumentation allows time resolution of seconds or milliseconds at best. Present capabilities are far removed from the natural time scale on which processes on a microscopic level occur which is between microseconds (µs) to attoseconds (as).In an effort to address current capability gaps, EMSL, with support from FCSD, the Fundamental & Computational Sciences Directorate at PNNL, organized an "Ultrafast Electron Microscopy Workshop," held June 14-15, 2011, with the primary goal to identify the scientific needs that could be met by creating a facility capable of a strongly improved time resolution with integrated in situ capabilities. The workshop brought together more than 40 leading scientists involved in applying and/or advancing electron microscopy to address important scientific problems of relevance to DOE's research mission. This workshop built on previous workshops 1 and included three breakout sessions identifying scientific challenges in biology, biogeochemistry, catalysis, and materials science-frontier areas of fundamental science that underpin energy and environmental science-that would significantly benefit from ultrafast transmission electron microscopy. In addition, the current status of time-resolved electron microscopy was examined, and the technologies that will enable future advances in spatio-temporal resolution were identified in a fourth breakout session.The major conclusion of the workshop was that significant scientific...

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Purification and Characterization of the [NiFe]-Hydrogenase of Shewanella oneidensis MR-1

Cited by 33 publications

References 44 publications

Dissimilatory Reduction of Extracellular Electron Acceptors in Anaerobic Respiration

Dissimilatory Reduction of Extracellular Electron Acceptors in Anaerobic Respiration

Macroporous monolithic Magnéli-phase titanium suboxides as anode material for effective bioelectricity generation in microbial fuel cells

Dynamic Processes in Biology, Chemistry, and Materials Science: Opportunities for UltraFast Transmission Electron Microscopy - Workshop Summary Report

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