Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility

Iqbal, Hafiz M.N.; Ahmed, Ishtiaq; Zia, Muhammad Anjum; Irfan, Muhammad

doi:10.4236/abb.2011.23024

Cited by 111 publications

(79 citation statements)

References 29 publications

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“…Stabilizers such as glycerol, for example, can increase the enzymatic stability (Singh et al 1991;Kapoor and Kuhad 2008) and the enzyme half-life during storage. In another study, an endoglucanase from T. viride showed a residual activity of 58 % after 30 days at 4 °C (Iqbal et al 2011). These data show that the enzyme produced by Melanoporia sp.…”

Section: Cellulase Stability During Storage At Low Temperaturesmentioning

confidence: 71%

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

Oliveira

Maciel

Sancho

et al. 2016

Bioresour. Bioprocess.

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Background:The use of residues in industrial processing is expanding due to their low cost and abundant availability. Coconut shell is generated in large amounts in tropical areas due to the fruit processing and the coconut water consumption. In the present work, a new microbial strain was isolated from the coconut shell powder, molecularly identified as Melanoporia sp. CCT 7736 and applied for cellulase production in solid-state fermentation using the green coconut shell powder as substrate. Results:The complete production process was optimized. Fermentation time was only 24 h, and the enzyme produced presented maximal activity at neutral pH (6.5) and 60 °C. The maximal enzyme activity after extraction optimization was 7.5 IU/gds (international units of enzyme activity per gram of dry solid). For the enzyme extraction, the rotation velocity, the extraction time, the temperature, and the solvent volume (buffer) were optimized using response surface methodology (RSM). The best results for the enzyme extraction were obtained at 250 rpm (orbital shaker) at 30 °C using 13.79 mL of a sodium acetate buffer (200 mM) at pH 6.5 after 10 min. Delignification pretreatment was not necessary since this fungus strain was able to degrade the lignin. Conclusions:To the best of our knowledge, this work is the first report of cellulase production by Melanoporia sp. CCT 7736. Good results were obtained without the need for expensive pretreatment usually applied to lignocellulosic residues because the strain was isolated from coconut shell powder, and it is well adapted to this kind of substrate. The enzyme presented maximum activity at neutral pH instead of acidic pH as reported for the majority of industrial cellulases. The use of lignified coconut shell and the optimal pH at neutral values are the main advantages of the enzyme produced by Melanoporia sp. CCT 7736. In addition, the enzyme showed good stability during storage even at the crude broth and without any cryoprotection.

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Section: Cellulase Stability During Storage At Low Temperaturesmentioning

confidence: 71%

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

Oliveira

Maciel

Sancho

et al. 2016

Bioresour. Bioprocess.

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“…The enzyme activity of the collected supernatants was measured using the method of Iqbal et al (2011). The Bradford (1976) assay was applied to determine the protein contents of the crude and purified enzyme using bovine serum albumin (BSA) as a standard.…”

Section: Enzyme Activity and Protein Contentsmentioning

confidence: 99%

“…A Sephadex-G 100 (Sigma-Aldrich, USA) with specifications of 120-cm height and 2.0-cm interior diameter (Sharma et al 2006) was used. Twenty fractions measuring 1 mL each were collected at a flow rate of 0.5 mL min −1 and analyzed for protein content and the determination of enzyme activity using the methodology of Iqbal et al (2011) andBradford (1976).…”

Section: Gel Filtration Chromatographymentioning

confidence: 99%

Detergent-Compatible Purified Endoglucanase from the Agro-Industrial Residue by Trichoderma harzianum under Solid State Fermentation

2016

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A robust process of purification, characterization, and application of endoglucanase from the agro-industrial waste was performed using solid state fermentation (SSF). Trichoderma harzianum as a micro-organism and wheat straw as a growth supportive substrate were used in SSF under pre-optimized conditions. The maximum activity of 480 ± 4.22 U/mL of endoglucanase was attained when a fermentation medium was inoculated using 10% inoculum size and 3% substrate concentration with pH = 5.5 at 35 °C for an optimized fermentation period. In comparison with crude extract, enzyme was 1.83-fold purified with a specific activity of 101.05 U/mg using Sephadex-G-100 column chromatography. Sodium dodecyl sulfate (SDS) poly-acrylamide gel electrophoresis revealed that the enzyme exhibited a low molecular weight of 43 kDa. The purified enzyme displayed maximum activity at pH = 6 and a temperature of 50 °C, respectively. The maximum activity (Vmax) of 156 U/mL and KM value of 63 µM were observed. Ethylenediaminetetraacetic acid (EDTA), SDS, and Hg 2+ inhibited enzyme activity, while Co 2+ and Mn 2+ enhanced enzyme activity at 1 mM concentration. The maximum substrate affinity and specific activity of biosynthesized endoglucanase revealed that it can be potentially useful for industrial applications.

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“…For biological treatments, the use of various fungi shows unique characteristics when the de-lignification of the lignocellulosic biomass produces residues. The white rot fungi (WRF) are the most efficient degraders of lignin due to their ligninolytic enzymes, such as lignin peroxidase (LiP), manganese peroxidase (MnP), laccase, and H2O2-producing oxidases (Iqbal et al 2011). These ligninolytic enzymes have low substrate specificity, non-stereo selectivity, and strong oxidative abilities (Iqbal et al 2011;Asgher et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The white rot fungi (WRF) are the most efficient degraders of lignin due to their ligninolytic enzymes, such as lignin peroxidase (LiP), manganese peroxidase (MnP), laccase, and H2O2-producing oxidases (Iqbal et al 2011). These ligninolytic enzymes have low substrate specificity, non-stereo selectivity, and strong oxidative abilities (Iqbal et al 2011;Asgher et al 2013). Among WRF, Ganoderma lucidum, Trametes versicolor, and Pleurotus ostreatus are efficient producers of ligninolytic enzymes (Xavier et al 2007;Batool et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Enzyme-treated Wheat Straw-based PVOH Bio-composites: Development and Characterization

Ahmad¹,

Bajwa²,

Iqbal³

2017

BioResources

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Valorization of lignocellulosic waste residues in the development of potential biodegradable composites has been of recent research interest. Recent research has shown that wheat straw can be used as a reinforcement material for the synthesis of novel polyvinyl alcohol (PVOH)-based composites. However, certain pretreatment methodology needs to be used for the selective removal of the lignin component. The delignification of native wheat straw was performed using an in-house isolated ligninolytic consortium. The bio-composites were developed using the de-lignified wheat straw along with PVOH as the matrix phase and glycerol as a plasticizer via a compression molding technique. In this study, a structural analysis by Fourier transform infrared spectroscopy (FT-IR) showed that the enzymatic treatment led to noticeable changes in the chemical structure of the materials used. A dynamic mechanical analysis (DMA) of the composites revealed an increase in the tensile strength of the sample from 46.1 MPa ± 0.1 MPa to 53.0 MPa ± 0.9 MPa, upon the addition of the plasticizer. Also, there was a noticeable increase in the tensile modulus of composites from 2,130 MPa to 4,520 MPa, respectively. Topographical features of the newly synthesized PVOHbased bio-composites were observed using scanning electron microscopy.

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Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility

Cited by 111 publications

References 29 publications

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

Detergent-Compatible Purified Endoglucanase from the Agro-Industrial Residue by Trichoderma harzianum under Solid State Fermentation

Enzyme-treated Wheat Straw-based PVOH Bio-composites: Development and Characterization

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