Purification and Characterization of the Amino-Terminal Domain of Lumazine Protein from Photobacterium leiognathi

Kim, Soyoung; Kim, Ryu-Ryun; Choi, Ji-Sun; Kim, Young-Doo; Lee, Chan Yong

doi:10.5012/bkcs.2010.31.04.1017

Bulletin of the Korean Chemical Society

2010

DOI: 10.5012/bkcs.2010.31.04.1017

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Purification and Characterization of the Amino-Terminal Domain of Lumazine Protein from Photobacterium leiognathi

Soyoung Kim¹,

Ryu-Ryun Kim²,

Ji-Sun Choi³

et al.

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2013

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Spectrofluorometric Properties of N-Terminal Domain of Lumazine Protein from Photobacterium leiognathi

Kang¹,

Kim²,

Lee³

et al. 2013

Bulletin of the Korean Chemical Society

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Lumazine protein is a member of the riboflavin synthase superfamily and the intense fluorescence is caused by non-covalently bound to 6,7-dimethyl 8-ribityllumazine. To figure out the binding modes and the structure of the N-terminal domain of lumazine protein, the wild type of protein extending to amino acid 118 (N-LumP 118 Wt) and mutants of N-LumP 118 V41W, S48W, T50W, D64W, and A66W from Photobacterium leiognathi were purified. The biochemical properties of the wild type and mutants of N-LumP 118 proteins were analyzed by absorbance and fluorescence spectroscope. The peak of absorbance and fluorescence of lumazine ligand were shifted to longer wavelength on binding to N-LumPs. The observed absorbance value at 410 nm of lumazine bound to N-LumP 118 proteins indicate that one mole of N-LumP 118 proteins bind to one mole of ligand of lumazine. Fluorescence analysis show that the maximum peak of fluorescence of N-LumP S48W was shifted to the longest wavelength by binding with 6,7-dimethyl 8-ribityllumazine and was shown to the greatest quench effect by acrylamide among all tryptophan mutants.

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Spectrofluorometric Properties of N-Terminal Domain of Lumazine Protein from Photobacterium leiognathi

Kang¹,

Kim²,

Lee³

et al. 2013

Bulletin of the Korean Chemical Society

Self Cite

View full text Add to dashboard Cite

show abstract

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Purification and Characterization of the Amino-Terminal Domain of Lumazine Protein from Photobacterium leiognathi

Cited by 1 publication

References 21 publications

Spectrofluorometric Properties of N-Terminal Domain of Lumazine Protein from Photobacterium leiognathi

Spectrofluorometric Properties of N-Terminal Domain of Lumazine Protein from Photobacterium leiognathi

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