Purification and characterization of the U-particle, a cellular constituent whose synthesis is stimulated by Mengovirus infection

Mulvey, Michael R.; Fang, Hengsheng; Scraba, Douglas G.

doi:10.1007/978-3-7091-9326-6_30

Positive-Strand RNA Viruses 1994

DOI: 10.1007/978-3-7091-9326-6_30

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Purification and characterization of the U-particle, a cellular constituent whose synthesis is stimulated by Mengovirus infection

Michael R. Mulvey

Hengsheng Fang

Douglas G. Scraba

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1996

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“…Other experiments have found that there is an increase in serum and red blood cell ferritin levels in patients infected with human immunodeficiency virus (36). The picornaviruses Mengo and Theiler's murine encephalitis have been shown to induce the synthesis of both L and H apoferritin proteins in infected mouse L929 fibroblasts and human HeLa cells (1,37,38). In contrast, no increase in ferritin production in HeLa cells infected with poliovirus or L929 cells infected with reovirus was observed.…”

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confidence: 98%

Induction of Ferritin Synthesis in Cells Infected with Mengo Virus

Mulvey

Kühn²,

Scraba

1996

Journal of Biological Chemistry

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We have recently identified ferritin as a cellular protein particle whose synthesis is stimulated in mouse or human cells infected by the picornavirus Mengo. Immunoprecipitation of the particle from infected murine L929 cells showed a 4- and 6-fold increase in the intracellular concentrations of H and L apoferritin subunits, respectively. This differential expression altered the H/L subunit ratio from 3.0 in uninfected cells to 2.2 in Mengo virus-infected cells. The induction is not due to an increase in transcription of the apoferritin L and H genes, nor is it due to an increase in stability of the apoferritin mRNAs. At the level of translation, the iron regulatory protein (IRP) remained intact, with similar amounts being detected in uninfected and infected cells. The Mengo virus RNA genome does not compete with the iron regulatory element (IRE) for the binding of IRP, and sequence analysis confirmed that there are no IREs in the virus RNA. The IRE binding activity of IRP in infected cells decreased approximately 30% compared with uninfected cells. The decrease in binding activity could be overcome by the addition of Desferal (deferoxamine mesylate; CIBA) an intracellular iron chelator, which suggests that virus infection causes an increase in intracellular free iron. Electron paramagnetic resonance (EPR) studies have confirmed the increase in free iron in Mengo virus infected cells. The permeability of cells for iron does not change in virus infected cells, suggesting that the induction of ferritin by Mengo virus is due to a change in the form of intracellular iron from a bound to a free state.

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mentioning

confidence: 98%

Induction of Ferritin Synthesis in Cells Infected with Mengo Virus

Mulvey

Kühn²,

Scraba

1996

Journal of Biological Chemistry

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Purification and characterization of the U-particle, a cellular constituent whose synthesis is stimulated by Mengovirus infection

Cited by 1 publication

References 4 publications

Induction of Ferritin Synthesis in Cells Infected with Mengo Virus

Induction of Ferritin Synthesis in Cells Infected with Mengo Virus

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