Purification and characterization of three novel keratinolytic metalloproteases produced by Chryseobacterium indologenes TKU014 in a shrimp shell powder medium

Wang, San-Lang; Hsu, Wan Ting; Liang, Tzu Wen; Yen, Yue Horng; Wang, Chuan Lu

doi:10.1016/j.biortech.2007.10.024

Cited by 76 publications

(45 citation statements)

References 31 publications

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“…There are few reports on the role of degraded feather lysate to promote plant growth as they are rich source of nitrogen (Bhange et al 2016;Kim et al 2001). Although the Chryseobacterium genus is known to have strong keratinase activity (Riffel et al 2007;Wang et al 2008); however, this is the first report on salinity stress amelioration using the combination of ACC deaminase producing organism with feather-degraded lysate.…”

Section: Discussionmentioning

confidence: 96%

Synergistic effect of Chryseobacterium gleum sp. SUK with ACC deaminase activity in alleviation of salt stress and plant growth promotion in Triticum aestivum L.

2017

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Soil salinity is major abiotic stresses affecting morphological, biochemical and physiological processes of plant growth. Chryseobacterium gleum sp. SUK isolated from salt-stressed soil exhibited ACC (1-aminocyclopropane-1-carboxylate) deaminase activity with IAA (indole acetic acid), siderophore, ammonia, hydrogen cyanide production, 2% salt tolerance and fungal cell wall degrading enzyme production (cellulase, protease). The isolate also showed a poultry feather degrading activity which is the main waste material of poultry industry and opulent source of proteins, amino acids, nitrogen, phosphorous, calcium, potassium, manganese, zinc and copper. Application of feather-degraded lysate with the degrading isolate, C. gleum sp. SUK denotes triple role of bioformulation to surmount salinity stress, management of poultry waste disposal and utilization of feathers degraded products as a biostimulant for better growth of plants as well as strain SUK having multifarious plant growth promoting traits. Wheat crops exposed to salt stressor were inoculated with studied bioformulation. Results of plant analysis showed improvement in root and shoot length, fresh and dry weight, chlorophyll, proteins, amino acids, phenolics, flavonoids content and decreased level of proline. In addition, Na ? uptake was decreased and K ? uptake was increased. Therefore, application of novel bioformulation could increase the yield of crops by ameliorating growth of plants and alleviating the salinity stress.

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Section: Discussionmentioning

confidence: 96%

Synergistic effect of Chryseobacterium gleum sp. SUK with ACC deaminase activity in alleviation of salt stress and plant growth promotion in Triticum aestivum L.

2017

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“…Although their overall clinical impact is lower than that of Enterobacteriaceae, Pseudomonas, or Acinetobacter species, Flavobacteriaceae may cause various clinical syndromes that are not always straightforward to treat, due mainly to their intrinsic resistance to several antimicrobial agents (29). Eight different sublineages of resident enzymes in members of the family Flavobacteriaceae have been identified thus far (4-7, 18, 20, 26), and most of them (BlaB from Elizabethkingia meningoseptica, IND from Chryseobacterium indologenes, JOHN from Flavobacterium johnsoniae, CGB from Chryseobacterium gleum, EBR from Empedobacter brevis, TUS from Myroides odoratus, and MUS from Myroides odoratimimus) belong to subclass B1, although the GOB enzymes (from E. meningoseptica) belong to subclass B3.The most common flavobacterium from clinical specimens is C. indologenes, which is associated with different types of infections, such as intra-abdominal and urinary tract infections, catheter-related bacteremia, cellulitis, sepsis, and pneumonia, likely promoted by the bacterium's ability to form biofilm and to produce proteases (1,3,15,16,21,23,24,33). In some nosocomial settings, C. indologenes infections were also associated with relatively high mortality rates (16).…”

mentioning

confidence: 99%

“…The most common flavobacterium from clinical specimens is C. indologenes, which is associated with different types of infections, such as intra-abdominal and urinary tract infections, catheter-related bacteremia, cellulitis, sepsis, and pneumonia, likely promoted by the bacterium's ability to form biofilm and to produce proteases (1,3,15,16,21,23,24,33). In some nosocomial settings, C. indologenes infections were also associated with relatively high mortality rates (16).…”

mentioning

confidence: 99%

IND-6, a Highly Divergent IND-Type Metallo-β-Lactamase fromChryseobacterium indologenesStrain 597 Isolated in Burkina Faso

Zeba

Luca

Dubus

et al. 2009

Antimicrob Agents Chemother

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The genus Chryseobacterium and other genera belonging to the family Flavobacteriaceae include organisms that can behave as human pathogens and are known to cause different kinds of infections. Several species of Flavobacteriaceae, including Chryseobacterium indologenes, are naturally resistant to ␤-lactam antibiotics (including carbapenems), due to the production of a resident metallo-␤-lactamase. Although C. indologenes presently constitutes a limited clinical threat, the incidence of infections caused by this organism is increasing in some settings, where isolates that exhibit multidrug resistance phenotypes (including resistance to aminoglycosides and quinolones) have been detected. Here, we report the identification and characterization of a new IND-type variant from a C. indologenes isolate from Burkina Faso that is resistant to ␤-lactams and aminoglycosides Metallo-␤-lactamases (MBLs) belong to Ambler's class B, and their activity requires at least one zinc ion in the active site. These enzymes are particularly worrisome resistance determinants due to their ability to hydrolyze most ␤-lactam compounds, including carbapenems, while they are not susceptible to conventional ␤-lactamase inhibitors (e.g., clavulanic acid, sulbactam, and tazobactam) (25). Besides the acquired MBL genes which currently disseminate in major human pathogens (e.g., Pseudomonas aeruginosa) by means of mobile genetic elements (plasmids, integrons, or ISCR elements), most MBL genes identified thus far have been found in the chromosomes of various bacterial species, including nonpathogenic organisms (such as Caulobacter crescentus), plant pathogens (Erwinia carotovora), and occasional human pathogens such as Stenotrophomonas maltophilia, Aeromonas spp., and members of the family Flavobacteriaceae (25, 32). The latter family includes several species in which the production of a resident MBL has been detected. Although their overall clinical impact is lower than that of Enterobacteriaceae, Pseudomonas, or Acinetobacter species, Flavobacteriaceae may cause various clinical syndromes that are not always straightforward to treat, due mainly to their intrinsic resistance to several antimicrobial agents (29). Eight different sublineages of resident enzymes in members of the family Flavobacteriaceae have been identified thus far (4-7, 18, 20, 26), and most of them (BlaB from Elizabethkingia meningoseptica, IND from Chryseobacterium indologenes, JOHN from Flavobacterium johnsoniae, CGB from Chryseobacterium gleum, EBR from Empedobacter brevis, TUS from Myroides odoratus, and MUS from Myroides odoratimimus) belong to subclass B1, although the GOB enzymes (from E. meningoseptica) belong to subclass B3.The most common flavobacterium from clinical specimens is C. indologenes, which is associated with different types of infections, such as intra-abdominal and urinary tract infections, catheter-related bacteremia, cellulitis, sepsis, and pneumonia, likely promoted by the bacterium's ability to form biofilm and to produce proteases (1,3,15,16,21,23,24...

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“…Utilizing natural residues, particularly in countries where they are generated in abundance, might hence considerably reduce the cost of enzyme production [36]. Furthermore, using agro-industrial substrates or environmental wastes might greatly bring down pollution problems [37].…”

Section: Bacillus Pumilus Strain Cicchlj Q89mentioning

confidence: 99%

A Bacillus Strain Able to Hydrolyze Alpha- and Beta-Keratin

Fellahi

Zaghloul²,

Feuk-Lagerstedt³

et al. 2014

J Bioproces Biotech

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The ability to hydrolyze keratin, a rigid and strongly cross-linked protein in the waste of poultry feather and sheep wool, has made keratinase production by microorganisms highly important to the biotechnological industry. A proteindegrading bacterium (C 4 ) was isolated from compost. Based on morphology and biochemical tests, along with 16S rRNA sequencing, the isolated C 4 was tentatively identified as Bacillus sp. C 4 (2008). The proteolytic activity of the Bacillus sp. C 4 strain was broadly specific; it degraded keratinous and non-keratinous proteins to different degrees. Pea pods as substrate generated the highest protease production, followed by soybean meal and sheep wool. Notwithstanding, using wool keratin as a sole source of carbon and nitrogen yielded the highest level of soluble proteins. Furthermore, the C 4 bacterium grew well, and produced a significant level of keratinase when using wool and feather as substrates. Supplementing the medium with yeast extract and peptone shortened the time required for feather degradation, but delayed the onset of the wool keratin hydrolysis with two days. The predominant amino acids released in feather hydrolysate were tyrosine, phenylalanine, and histidine. In contrast, the wool lysate was rich in aspartic acid, methionine, tyrosine, phenylalanine, histidine, and lysine. Results established that utilizing the C 4 strain for keratin degradation in waste management holds considerable potential.

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Purification and characterization of three novel keratinolytic metalloproteases produced by Chryseobacterium indologenes TKU014 in a shrimp shell powder medium

Cited by 76 publications

References 31 publications

Synergistic effect of Chryseobacterium gleum sp. SUK with ACC deaminase activity in alleviation of salt stress and plant growth promotion in Triticum aestivum L.

Synergistic effect of Chryseobacterium gleum sp. SUK with ACC deaminase activity in alleviation of salt stress and plant growth promotion in Triticum aestivum L.

IND-6, a Highly Divergent IND-Type Metallo-β-Lactamase fromChryseobacterium indologenesStrain 597 Isolated in Burkina Faso

A Bacillus Strain Able to Hydrolyze Alpha- and Beta-Keratin

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