Purification and Characterization of Two Novel Laccases from Peniophora lycii

Abstract

Although, currently, more than 100 laccases have been purified from basidiomycete fungi, the majority of these laccases were obtained from fungi of the Polyporales order, and only scarce data are available about the laccases from other fungi. In this article, laccase production by the white-rot basidiomycete fungus Peniophora lycii, belonging to the Russulales order, was investigated. It was shown that, under copper induction, this fungus secreted three different laccase isozymes. Two laccase isozymes—Lac5 and LacA—were purified and their corresponding nucleotide sequences were determined. Both purified laccases were relatively thermostable with periods of half-life at 70 °C of 10 and 8 min for Lac5 and LacA, respectively. The laccases demonstrated the highest activity toward ABTS (97 U·mg−1 for Lac5 and 121 U·mg−1 for LacA at pH 4.5); Lac5 demonstrated the lowest activity toward 2,6-DMP (2.5 U·mg−1 at pH 4.5), while LacA demonstrated this towards gallic acid (1.4 U·mg−1 at pH 4.5). Both Lac5 and LacA were able to efficiently decolorize such dyes as RBBR and Bromcresol Green. Additionally, phylogenetic relationships among laccases of Peniophora spp. were reconstructed, and groups of orthologous genes were determined. Based on these groups, all currently available data about laccases of Peniophora spp. were systematized.

Comparative Analysis of Peniophora lycii and Trametes hirsuta Exoproteomes Demonstrates “Shades of Gray” in the Concept of White-Rotting Fungi

“…2 GP—control GP medium; AL— GP+Alder medium; BR— GP+Birch medium; PN— GP+Pine medium. 3 Naming of laccase and POD isozymes are in accordance with [ 19 , 20 ] for T. hirsuta LE-BIN 072 and [ 21 ] for P. lycii LE-BIN 2142.…”

High-Resolution Screening for Marine Prokaryotes and Eukaryotes With Selective Preference for Polyethylene and Polyethylene Terephthalate Surfaces

“…12 fungal OTUs were found only on the plastic samples including the species Pyrenochaetopsis leptospora , Candida sake , Debaryomyces vindobonensis, Aspergillus penicillioides , and Bipolaris sorokiniana , a wheat pathogen that causes leaf spot disease ( Ye et al, 2019 ). Peniophora lycii , a species that was found on both PE and PET, but not on glass or water, has recently been shown to secrete three laccase isozymes ( Glazunova et al, 2020 ), that may be capable of breaking down non-hydrolyzable plastics such as PE ( Inderthal et al, 2021 ). Many marine fungi can degrade complex hydrocarbons [reviewed in Amend et al (2019) ] and often dominate in oil polluted environments ( Bik et al, 2012 ; McGenity et al, 2012 ).…”

High-resolution screening for marine prokaryotic and eukaryotic taxa with selective preference for PE and PET surfaces

“…12 fungal OTUs were found to be unique to the plastic samples including the species Pyrenochaetopsis leptospora, Candida sake, Debaryomyces vindobonensis, Aspergillus penicillioides and Bipolaris sorokiniana, a wheat pathogen that causes leaf spot disease (Ye et al, 2019). Peniophora lycii, species that was found on both PE and PET, but not on glass or water, has recently been shown to secrete three laccase isozymes (Glazunova et al, 2020), that may be capable of breaking down non-hydrolysable plastics such as PE (Inderthal et al, 2021). Many marine fungi can degrade complex hydrocarbons (reviewed in (Amend et al, 2019)) and often dominate in oil polluted environments (Bik et al, 2012;McGenity et al, 2012).…”