Purification and characterization of two extracellular peroxidases from Streptomyces sp. strain AM2, a decolorizing actinomycetes responsible for the biodegradation of natural humic acids

Fodil, Djamila; Badis, Abdelmalek; Jaouadi, Bassem; Zaraî, Nedia; Ferradji, Fatma Zohra; Boutoumi, Hocine

doi:10.1016/j.ibiod.2011.01.009

Cited by 31 publications

(17 citation statements)

References 38 publications

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“…These values are consistent with previous studies, where PO was recovered from several sources with optimal temperature between 30 and 50 °C. 1,[20][21][22] For both forms of PO, the pH that presented the greatest enzymatic activity was 5, a value consistent with findings when purified from Streptomyces sp 23 and Leucaena leucocephala. 24 In the evaluation of pH, there weren't significant differences in reaction pH of the crude extract between 4.5 and 6 and of the pure extract between 5 and 6.…”

Section: Optimal Reaction Conditionssupporting

confidence: 84%

Peroxidase from soybean meal: obtention, purification and application in reduction of deoxynivalenol levels

Feltrin¹,

Fontes²,

Gracia³

et al. 2017

Quim. Nova

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This study established the conditions for the extraction of the enzyme peroxidase (PO) from soybean meal (SBM). An experimental design methodology was carried out in order to evaluate the effects of stirring rate time, pH and extracting solvent volume on the enzyme extraction. By using 5 g SBM and 50 mL phosphate buffer 10 mmol L -1 pH 4.7, 60 min stirring rate at 100 rpm, an enzyme with specific activity of 100 U mg -1 for SBM was obtained. Two techniques of purification were tested and compared for purification of peroxidase from soybean meal: triphasic partition (TPP) and molecular exclusion chromatography. TPP showcased a greater efficiency with 50% recuperation and a purification factor of 13.6. Peroxidase in crude and pure forms was characterized for kinetics, thermodynamics and biochemistry. The parameter of thermal inactivation indicates high stability to exposure time and temperature increase, showing that enzyme activity is not altered by the presence of constituents of the reaction medium. Peroxidase in crude form represented a greater upkeep in activity, keeping 50% activity for 114 days at 0 °C. Peroxidase in pure form had greater affinity for substrate and reduced Deoxynivalenol levels by 80%, 20% more than the crude form.

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Section: Optimal Reaction Conditionssupporting

confidence: 84%

Peroxidase from soybean meal: obtention, purification and application in reduction of deoxynivalenol levels

Feltrin¹,

Fontes²,

Gracia³

et al. 2017

Quim. Nova

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“…The spectral characteristics of the purified peroxidase illustrated that LiP-SN had an amino-acid peak at 280 nm and a heme peak at 409 nm (data not shown). The deduced Reinheitzahl (RZ) value was 1.63, which was comparable to those previously reported for other peroxidases [9][10][11]13,22]. These data strongly suggested that LiP-SN contained a heme cofactor that was in an oxidized state.…”

Section: Enzyme Purificationsupporting

confidence: 84%

“…Moreover, and despite the large flow of data on peroxidases, only few studies have so far reported on the purification and characterization of peroxidases from Streptomyces [9][10][11][12][13]. Additionally, and to the authors' knowledge, no previous work has so far been performed to investigate the incorporation of peroxidase in detergent formulations.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9

Rekik

Nadia

Bejar

et al. 2015

International Journal of Biological Macromolecules

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“…All these enzymes together contribute to its biocontrol potential against a wide range of phytopathogens, because the cell wall of most fungal and bacterial pathogens consist of polymers such as chitin, glucan, cellulose, proteins, and lipids (Gupta et al 1995;Fodil et al 2011). Among them, chitinases are of great importance and many Streptomyces spp.…”

Section: Cell Wall Degrading Enzymesmentioning

confidence: 99%

Plant growth-promoting actinobacteria: a new strategy for enhancing sustainable production and protection of grain legumes

2017

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Grain legumes are a cost-effective alternative for the animal protein in improving the diets of the poor in South-East Asia and Africa. Legumes, through symbiotic nitrogen fixation, meet a major part of their own N demand and partially benefit the following crops of the system by enriching soil. In realization of this sustainability advantage and to promote pulse production, United Nations had declared 2016 as the ''International Year of pulses''. Grain legumes are frequently subjected to both abiotic and biotic stresses resulting in severe yield losses. Global yields of legumes have been stagnant for the past five decades in spite of adopting various conventional and molecular breeding approaches. Furthermore, the increasing costs and negative effects of pesticides and fertilizers for crop production necessitate the use of biological options of crop production and protection. The use of plant growth-promoting (PGP) bacteria for improving soil and plant health has become one of the attractive strategies for developing sustainable agricultural systems due to their eco-friendliness, low production cost and minimizing consumption of non-renewable resources. This review emphasizes on how the PGP actinobacteria and their metabolites can be used effectively in enhancing the yield and controlling the pests and pathogens of grain legumes.

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Purification and characterization of two extracellular peroxidases from Streptomyces sp. strain AM2, a decolorizing actinomycetes responsible for the biodegradation of natural humic acids

Cited by 31 publications

References 38 publications

Peroxidase from soybean meal: obtention, purification and application in reduction of deoxynivalenol levels

Peroxidase from soybean meal: obtention, purification and application in reduction of deoxynivalenol levels

Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9

Plant growth-promoting actinobacteria: a new strategy for enhancing sustainable production and protection of grain legumes

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