Purification and characterization of tyrosinase from walnut leaves (Juglans regia)

Zekiri, Florime; Molitor, Christian; Mauracher, S.G.; Michael, Claudia; Mayer, Rupert L.; Gerner, Christopher; Rompel, Annette

doi:10.1016/j.phytochem.2014.02.010

Cited by 78 publications

(86 citation statements)

References 46 publications

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“…By applying the method described by Zekiri et al (2014), the enzyme was purified to homogeneity. Initial attempts to crystallize the most abundant form jrPPO1(Asp 101 -Arg 445 ), as described in Zekiri et al (2014), covering a wide range of crystallization conditions proved to be very successful.…”

Section: Resultsmentioning

confidence: 99%

“…Initial attempts to crystallize the most abundant form jrPPO1(Asp 101 -Arg 445 ), as described in Zekiri et al (2014), covering a wide range of crystallization conditions proved to be very successful. Crystals suitable for X-ray diffraction experiments were obtained using 30% PEG 5000 MME, 200 mM ammonium sulfate, 100 mM MES pH 6.5 (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The isolation and purification process of the enzyme has been described in detail by Zekiri et al (2014). Briefly, extraction of the enzyme from the natural source (walnut leaves) was performed using the method developed by Mauracher, Molitor, Michael et al (2014) based on detergent and soluble polymer polyethylene glycol (PEG) phase separations.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Recently, a tyrosinase from walnut leaves (Juglans regia; jrPPO1; UniProt COLU17) was isolated and characterized as having a monophenolase activity (Escobar et al, 2008;Araji et al, 2014;Zekiri et al, 2014), thus making jrPPO1 an attractive enzyme for crystallographic studies of a plant tyrosinase. Although it exhibits monophenolase activity, jrPPO1 has the highest sequence identity to catechol oxidase from V. vinifera (sequence identity 62%; UniProt P4331; Virador et al, 2010) and has only weak identity (<20%) to bacterial and fungal tyrosinases (Matoba et al, 2006;Ismaya et al, 2011;Sendovski et al, 2011;Mauracher, Molitor, Michael et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Crystallization and preliminary X-ray crystallographic analysis of polyphenol oxidase fromJuglans regia(jrPPO1)

et al. 2014

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Tyrosinase is a type 3 copper enzyme that catalyzes the ortho-hydroxylation of monophenols to diphenols as well as their subsequent oxidation to quinones, which are precursors for the biosynthesis of melanins. The first plant tyrosinase from walnut leaves (Juglans regia) was purified to homogeneity and crystallized. , and diffracted to 2.39 Å resolution. Crystals were only obtained from solutions containing at least 30% polyethylene glycol 5000 monomethyl ether in a close-to-neutral pH range.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Crystallization and preliminary X-ray crystallographic analysis of polyphenol oxidase fromJuglans regia(jrPPO1)

et al. 2014

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“…In connection with the discovery of the JrPPO genes, a tyrosinase was isolated from J. regia leaves and identified as a PPO corresponding to the known JrPPO1 sequence (Zekiri et al 2014). Tyrosinases can catalyze both ortho-hydroxylation of monophenols to odiphenols (monophenolase activity) and the subsequent oxidation of o-diphenols to the corresponding o-quinones (diphenolase activity).…”

Section: Proteomics Researchmentioning

confidence: 99%

Walnut: past and future of genetic improvement

Bernard

Lheureux²,

Dirlewanger

2017

Tree Genetics & Genomes

163

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Persian or English walnut (Juglans regia L.), the walnut species cultivated for nut production, is one of the oldest food sources known. Persian walnuts, native to the mountain valleys of Central Asia, are grown worldwide in temperate areas. World production exceeds three million tons since 2012, mostly provided by China, the USA, and Iran. Despite very ancient culture of walnut species (Juglans spp.), breeding actually started in the twentieth century. Using a range of methodologies, from morphological markers to the most recent advances in genome analysis, many genetic studies of walnut have been conducted during the past 30 years, including examination of diversity, determination of relationships within or among germplasm collections and populations, phylogenetic and origin elucidation, genetic map construction, and biotic or abiotic stress investigations. The genetic improvement of walnut has undergone great evolution. The producing countries of the Middle East have widely studied morphological characteristics of walnut. The USA and France, for example, are behind important cultivar releases such as BChandler^and BFranquette.^Finally, genomics represents a major breakthrough in walnut improvement, in particular by recent sequencing of both chloroplast and nuclear genomes. This review summarizes worldwide molecular and Bomics^studies and gives an overview of the main walnut breeding programs.

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Kristallstruktur einer pflanzlichen Tyrosinase aus Walnussblättern: die Bedeutung “substratlenkender Aminosäurereste” für die Enzymspezifität

et al. 2015

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Tyrosinasen und Catecholoxidasen gehçren zur Klasse der Typ-III-Kupferenzyme.F orscher arbeiten seit Jahrzehnten daran, die Monophenolase-/Diphenolase-Spezifität dieser Enzyme auf struktureller Ebene zu erklären und entwickelten dazu eine Hypothese (basierend auf der ersten Catechol-und Tyrosinasestruktur), der zufolge der Grund für das Fehlen von Monophenolase-Aktivität in Catecholoxidasen das strukturell eingeschränkte aktive Zentrum sein kçnnte. Jüngste strukturelle und biochemische Studien an dieser Enzymklasse liefern jedochAnlass zu Zweifeln an dieser Theorie. Hier wird die erste Kristallstruktur einer pflanzlichen Tyrosinase (Juglans regia) präsentiert, anhand derer gezeigt wird, dass die Unterscheidung zwischen Mono-und Diphenolase-Aktivität auf struktureller Ebene nicht durch eine strukturelle Einschränkung des aktiven Zentrums bestimmt wird;vielmehr spielen Aminosäuren, die sich am Eingang zu und innerhalb der zweiten Schale um das aktive Zentrum befinden, eine viel wichtigere Rolle,a ls bisher angenommen.

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Purification and characterization of tyrosinase from walnut leaves (Juglans regia)

Cited by 78 publications

References 46 publications

Crystallization and preliminary X-ray crystallographic analysis of polyphenol oxidase fromJuglans regia(jrPPO1)

Crystallization and preliminary X-ray crystallographic analysis of polyphenol oxidase fromJuglans regia(jrPPO1)

Walnut: past and future of genetic improvement

Kristallstruktur einer pflanzlichen Tyrosinase aus Walnussblättern: die Bedeutung “substratlenkender Aminosäurereste” für die Enzymspezifität

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