1997
DOI: 10.1128/jb.179.2.323-329.1997
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Purification and characterization of VSH-1, a generalized transducing bacteriophage of Serpulina hyodysenteriae

Abstract: Serpulina hyodysenteriae B204 cells treated with mitomycin (20 g of mitomycin/ml of culture broth) lysed and released bacteriophages. Bacteriophage particles, precipitated by using polyethylene glycol and purified by CsCl density gradient ultracentrifugation, had a buoyant density of 1.375 g/cm 3 and consisted of a head (45-nm diameter) and an ultrastructurally simple (noncontractile) tail (64 by 9 nm) composed of at least 13 proteins with molecular masses ranging between 13 and 101 kDa. The purified bacteriop… Show more

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Cited by 116 publications
(120 citation statements)
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“…2c), and contain 7.5 kb of linear double-stranded DNA 50 . The element is named VSH-1, and the packaged DNA is a random collection of fragments from the genome of the producing cell 50 .…”
Section: The D Desulfuricans Gtamentioning
confidence: 99%
See 4 more Smart Citations
“…2c), and contain 7.5 kb of linear double-stranded DNA 50 . The element is named VSH-1, and the packaged DNA is a random collection of fragments from the genome of the producing cell 50 .…”
Section: The D Desulfuricans Gtamentioning
confidence: 99%
“…2c), and contain 7.5 kb of linear double-stranded DNA 50 . The element is named VSH-1, and the packaged DNA is a random collection of fragments from the genome of the producing cell 50 . VSH-1 mediates the transfer of many markers between B. hyodysenteriae cells [50][51][52][53] .…”
Section: The D Desulfuricans Gtamentioning
confidence: 99%
See 3 more Smart Citations