Purification and characterization of xylanases from Trichoderma

Gerber, Paul; Heitmann, John A.; Joyce, T. W.

doi:10.1016/s0960-8524(97)00052-7

Cited by 15 publications

(10 citation statements)

References 42 publications

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“…Supplementation of nitrogen source is not always essential in the SSF system, as it depends on the availability of nitrogen in the substrate and requirement of the organisms. Xylanases with minimal cellulase can be produced by the low C:N source [32].…”

Section: Effect Of Different Nitrogen Sources On Xylanase Productionmentioning

confidence: 99%

Production of xylanase under solid-state fermentation by Aspergillus tubingensis JP-1 and its application

Pandya¹,

Gupte²

2012

Bioprocess Biosyst Eng

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The production of extracellular xylanase by a locally isolated strain of Aspergillus tubingensis JP-1 was studied under solid-state fermentation. Among the various agro residues used wheat straw was found to be the best for high yield of xylanase with poor cellulase production. The influence of various parameters such as initial pH, moisture, moistening agents, nitrogen sources, additives, surfactants and pretreatment of substrates were investigated. The production of the xylanase reached a peak in 8 days using untreated wheat straw with modified MS medium, pH 6.0 at 1:5 moisture level at 30 °C. Under optimized conditions yield as high as 6,887 ± 16 U/g of untreated wheat straw was achieved. Crude xylanase was used for enzymatic saccharification of agro-residues like wheat straw, rice bran, wheat bran, sugarcane bagasse and industrial paper pulp. Dilute alkali (1 N NaOH) and acid (1 N H(2)SO(4)) pretreatment were found to be beneficial for the efficient enzymatic hydrolysis of wheat straw. Dilute alkali and acid-pretreated wheat straw yielded 688 and 543 mg/g reducing sugar, respectively. Yield of 726 mg/g reducing sugar was obtained from paper pulp after 48 h of incubation.

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Section: Effect Of Different Nitrogen Sources On Xylanase Productionmentioning

confidence: 99%

Production of xylanase under solid-state fermentation by Aspergillus tubingensis JP-1 and its application

Pandya¹,

Gupte²

2012

Bioprocess Biosyst Eng

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“…Moreover it has been shown that the degree of selectivity for the production of a particular enzyme may be regulated by the carbon to nitrogen ratio. Xylanases with minimal cellulases can be produced by using a low nitrogen-to-carbon ratio (Gerber et al 1997). In the present study, combinations of organic and inorganic nitrogen sources have been studied by replacing the original sources of nitrogen in the modified MS medium (Table 3).…”

Section: Influence Of Nitrogen Sourcesmentioning

confidence: 99%

Xylanase production under solid-state fermentation and its characterization by an isolated strain of Aspergillus foetidus in India

Shah

Madamwar

2005

World J Microbiol Biotechnol

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A locally isolated strain of Aspergillus foetidus MTCC 4898 was studied for xylanase (EC 3.2.1.8) production using lignocellulosic substrates under solid state fermentation. Corncobs were found as the best substrates for high yield of xylanases with poor cellulase production. The influence of various parameters such as temperature, pH, moistening agents, moisture level, nitrogen sources and pretreatment of substrates were evaluated with respect to xylanase yield, specific activity and cellulase production. Influence of nitrogen sources on protease secretion was also examined. Maximum xylanase production (3065 U/g) was obtained on untreated corncobs moistened with modified Mandels and Strenberg medium, pH 5.0 at 1:5 moisture levels at 30°C in 4 days of cultivation. Submerged fermentation under the same conditions gave higher yield (3300 U/g) in 5 days of cultivation, but productivity was less. Ammonium sulphate fractionation yielded 3.56-fold purified xylanase with 76% recovery. Optimum pH and temperature for xylanase activity were found to be 5.3 and 50°C respectively. Kinetic parameters like K m and V max were found to be 3.58 mg/ml and 570 lmol/mg/min. Activity of the enzyme was found to be enhanced by cystiene hydrochloride, CoCl 2 , xylose and Tween 80, while significantly inhibited by Hg ++ , Cu ++ and glucose. The enzyme was found to be stable at 40°C. The half life at 50°C was 57.53 min. However thermostability was enhanced by glycerol, trehalose and Ca ++ . The crude enzyme was stable during lyophilization and could be stored at less than 0°C.

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“…Studies with T. reesei have reported two bands with apparent molecular masses of 19 and 20 kDa [29], and 23 and 32 kDa [30]. According to Chen et al [31] only one endoxylanase (37.7 kDa) was observed for T. longibrachiatum and Ang et al [24] detected three endoxylanase (45.7 kDa, 39.8 kDa and 18.2 kDa) produced by Aspergillus fumigatus SK1.…”

Section: Discussionmentioning

confidence: 97%

Trichoderma atroviride 102C1 Mutant: A High Endoxylanase Producer for Assisting Lignocellulosic Material Degradation

Oliveira¹

2014

Microb Biochem Technol

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Endoxylanases have played an important role in many industrial processes as bleachers to kraft pulp, animal feeds and baked goods. Also, nowadays, a special attention has been devoted to the role of these enzymes in saccharification of lignocellulose biomass for biofuels production. Trichoderma species are among fungi those that have been most extensively studied, due to their efficient production of these enzymes. Among the different strategies for improving the production and biochemical aspects of enzymes of commercial interest, mutations induced using chemical agents and/or physical devices can be cited. In the present strain T. atroviride 102C1 was obtained by using UV light and nitrosoguanidine as mutagenic agents. A factorial design (central composite rotational design, CCRD) was performed to estimate the optimal levels of C (sugarcane bagasse) and N (corn steep liquor) sources for best xylanase production. After the CCRD, the 102C1 mutant strain showed increased activity of 340% for xylanase production when compared to the wild type. The enzyme was partially characterized according to its pH and temperature profile, also using CCRD. The characterization of 102C1 mutant strain as a high endoxylanase producer allows its use in biotechnological applications, particularly in the hydrolysis of lignocellulosic biomass for biorefinary proposes.

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Purification and characterization of xylanases from Trichoderma

Cited by 15 publications

References 42 publications

Production of xylanase under solid-state fermentation by Aspergillus tubingensis JP-1 and its application

Production of xylanase under solid-state fermentation by Aspergillus tubingensis JP-1 and its application

Xylanase production under solid-state fermentation and its characterization by an isolated strain of Aspergillus foetidus in India

Trichoderma atroviride 102C1 Mutant: A High Endoxylanase Producer for Assisting Lignocellulosic Material Degradation

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