Purification and characterization of β-Fructosidase with inulinase activity from Aspergillus niger - 245

Cruz, Vinı́cius D'Arcadia; Belote, Juliana Gisele; Dorta, Cláudia; Santos, Luíza Helena Oliveira dos; Andriolo, Cláudia Regina; Khenayfes, Marcelo de Oliveira; Cruz, Rubens

doi:10.1590/s1516-89131998000300003

Braz. arch. biol. technol.

1998

DOI: 10.1590/s1516-89131998000300003

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Purification and characterization of β-Fructosidase with inulinase activity from Aspergillus niger - 245

Vinı́cius D'Arcadia Cruz

Juliana Gisele Belote

Cláudia Dorta

et al.

Abstract: ABSTRACT

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2021

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“…Also, at 70ºC, the lipase activity decreased to about 40% within 120 minutes of incubation. This was similar to the in which lipase production showed maximum activity at 50°C in et al [36] also reported that the enzyme activity declined as the temperature was gradually increased from 30 o C. This is because temperature affects the metabolic activity of microbial cells. Etabilli and ] also reported that denaturation of enzymes occurs when the temperature is high.…”

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confidence: 86%

Lipolytic Activities of Bacteria and Fungi Isolated from Soil Samples

Fashogbon

Adebayo

Musa

et al. 2021

MRJI

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This study was carried out at the Department of Microbiology, Microbiology Laboratory, Ado-Ekiti State University, Ekiti State, Nigeria between July, 2018 to March, 2019. Due to the diverse biotechnological importance of lipases as a biocatalytic enzyme, extracellular production of microbial lipases has to gain lots of interest. This study, therefore, focused on the physicochemical parameters of lipase producing microorganisms from different soil samples. Microorganisms were isolated from four different soil samples using Nutrient Agar (NA) and Potato Dextrose Agar (PDA). The isolates were identified and characterized. Production, an assay for Lipase enzymes, purification, the effect of pH, Temperature and metal ion was investigated. The isolates were culturally, morphologically and biochemically characterized. Two of the bacteria strains (Bacillus sp. and Staphylococcus sp.) and four fungi (Fusarium sp., Aspergillus fumigatus, Aspergillus niger, and Trichophyton sp.) isolates were able to produce lipid using Sudan Black B Fat staining techniques. Fusarium sp. isolated from dumpsite soil had the highest specific lipase activity (21.16 µmol/min/ml) while Bacillus sp. isolated from red oil spill soil had the highest lipase activity (0.59 µmol/min/mg). The specific activity of partially purified lipase for Fusarium sp. was 2.39 µmol/min/mg while Bacillus sp. had a specific activity of 2.46 µmol/min/mg. 30oC - 50oC, pH 7.0 to 9.0 and KCl2 (139.672%) supported the highest production of lipase by the Bacillus sp. and Fusarium sp. This study demonstrated that the Bacillus sp. produced a high amount of lipase activity followed by Fusarium sp. Extensive and persistent screening for new microorganisms and their lipolytic activities will help to provide faster ways to solve most environmental soil pollution.

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supporting

confidence: 86%

Lipolytic Activities of Bacteria and Fungi Isolated from Soil Samples

Fashogbon

Adebayo

Musa

et al. 2021

MRJI

View full text Add to dashboard Cite

show abstract

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Purification and characterization of β-Fructosidase with inulinase activity from Aspergillus niger - 245

Abstract: ABSTRACT

Cited by 1 publication

References 22 publications

Lipolytic Activities of Bacteria and Fungi Isolated from Soil Samples

Lipolytic Activities of Bacteria and Fungi Isolated from Soil Samples

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