Purification and Concentration of the Total Saikosaponins Extracted from<i>Radix Bupleuri</i>using Foam Fractionation

Xiao, Zongyuan; Li, Xianrong; Shao, Wenyao; Wu, Shuqi

doi:10.1080/01496395.2013.837922

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…Radix bupleuri rhizomes were purchased from Shanghai State-owned Changning Pharmacy (Shanghai, China); Saikosaponin A was isolated and characterized according to a previous report with slight modification [ 30 ]. 1000-g rhizomes of Radix bupleuri were taken, crushed, sieved through a 20-30-mesh sieve, soaked with 100% ethanol for three days, filtered, combined with the soaking solution, and evaporated under reduced pressure on a rotary evaporator to recover ethanol.…”

Section: Methodsmentioning

confidence: 99%

Saikosaponin A-Induced Gut Microbiota Changes Attenuate Severe Acute Pancreatitis through the Activation of Keap1/Nrf2-ARE Antioxidant Signaling

Han

et al. 2020

Oxidative Medicine and Cellular Longevity

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Objective. Severe acute pancreatitis (SAP) is a serious and life-threatening disease associated with multiple organ failure and a high mortality rate and is accompanied by distinct oxidative stress and inflammatory responses. Saikosaponin A has strong antioxidant properties and can affect the composition of gut microbiota. We sought to determine the effects of Saikosaponin A interventions on SAP by investigating the changes of gut microbiota and related antioxidant signaling. Methods. A SAP model was established in Sprague-Dawley (SD) rats through the injection of sodium taurocholate into the biliopancreatic duct and confirmed by elevated levels of serum lipase and amylase. The model was fed a standard diet either with saline solution or with Saikosaponin A. Fecal microbiota transplantation (FMT) from Saikosaponin A-induced rats into the rat model was performed to test the effects of gut microbiota. The composition of gut microbiota was analyzed by using 16S rRNA gene sequencing. We measured apoptotic status, inflammatory biomarkers, and Keap1-Nrf2-ARE ((Kelch-like ECH-associated protein 1) nuclear factor erythroid 2-related factor 2-antioxidant response element) antioxidant signaling. Results. Saikosaponin A intervention attenuated SAP lesions and reduced the levels of serum amylase and lipase, oxidative stress, and inflammatory responses by reducing pathological scores and affecting the serum level of oxidative and inflammatory factors. Meanwhile, the expression of Keap1-Nrf2-ARE was increased. Saikosaponin A intervention improved microbiota composition by increasing the relative abundance of Lactobacillus and Prevotella species. FMT resulted in similar results as those caused by the Saikosaponin A intervention, suggesting Saikosaponin A may exert its function via the improvement of gut microbiota composition. Conclusions. Saikosaponin A-induced gut microbiota changes attenuate SAP progression in the rat model and may be a potential natural drug for adjuvant treatment of SAP. Further work is needed to clear up the points.

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Section: Methodsmentioning

confidence: 99%

Saikosaponin A-Induced Gut Microbiota Changes Attenuate Severe Acute Pancreatitis through the Activation of Keap1/Nrf2-ARE Antioxidant Signaling

Han

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…The pH under 5 was not taken into account because it was detrimental for polymyxin E fermentation. Many researchers demonstrated the best pH for protein foam separation was at its isoelectric point (PI), [17][18][19] because the repulsive force between proteins was the lowest at PI. Therefore, proteins were prone to adsorb on gas-liquid interfaces.…”

Section: Part 2: Fermentation Coupling With Foam Separationmentioning

confidence: 99%

Process improvement for fermentation coupling with foam separation: a convenient strategy for cell recycle

Zhang

Dong

et al. 2015

Asia-Pacific J Chem Eng

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Applications of fermentation coupling with foam separation technology show great advantages in many natural biosurfactants fermentation such as nisin, surfactin, and polymyxin E. However, only a few reports focus on process improvement, i.e. cells overflowing with foam. Because the cells also have hydrophobicity, it will overflow during foam separation, which decrease the productivity and contaminate the environment. Therefore, it is important to prevent cell from overflowing. In this study, a new approach was proposed to increase yield, using fermentation coupling with foam separation technology. Meanwhile, we tried to prevent cell from overflowing. This strategy was characterized by enrichment, recovery percentage, and inactivity percentage for product and cells. The results indicated pH was the most important factor in foam separation of polymyxin E. The optimal condition for fermentation coupling with foam separation was determined. The maximum total titer of polymyxin E reached 917 932.31 U (1147.4 U/mL) using coupling strategy that was 1.36 times more than total titer in shaken-flask culture. Additionally, the adsorption mechanism for polymyxin E and cells was studied. The results indicated polymyxin E were mainly adsorbed on bubble, while the cells were partly in liquid form and partly adsorbed on undesired protein. Undesired protein can be considered as a stabilizer in foam separation of polymyxin E.

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“…Compared with other separation methods, foam fractionation is an interesting method owing to its advantages of simple equipment, low energy consumption, less investment and free pollution. It is specially suitable for separating trace materials from their solutions . Rujirawanich et al and Moussavi et al used SDS as the collector to remove C d 2+ and Hg + from their simulated wastewaters.…”

Section: Introductionmentioning

confidence: 99%

Recovery of streptomycin sulfate from the wastewater using foam fractionation coupled with adsorption separation for reusing sodium dodecyl sulfate

Kou

Zhao

et al. 2014

J of Chemical Tech & Biotech

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BACKGROUND: Surfactants are always used as collectors to form complexes with the hazardous materials of wastewaters in foam fractionation. An important topic is how to reuse the surfactants in the process of recovering the hazardous materials. In this work, a novel technology of foam fractionation coupled with adsorption separation was developed for separating streptomycin sulfate (SS) from its simulated wastewater and reusing the collector sodium dodecyl sulfate (SDS).

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Purification and Concentration of the Total Saikosaponins Extracted fromRadix Bupleuriusing Foam Fractionation

Cited by 5 publications

References 32 publications

Saikosaponin A-Induced Gut Microbiota Changes Attenuate Severe Acute Pancreatitis through the Activation of Keap1/Nrf2-ARE Antioxidant Signaling

Saikosaponin A-Induced Gut Microbiota Changes Attenuate Severe Acute Pancreatitis through the Activation of Keap1/Nrf2-ARE Antioxidant Signaling

Process improvement for fermentation coupling with foam separation: a convenient strategy for cell recycle

Recovery of streptomycin sulfate from the wastewater using foam fractionation coupled with adsorption separation for reusing sodium dodecyl sulfate

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