Purification and expression analysis of imaginal disc growth factor in the silkworm, Bombyx mori

Wang, Huabing; Sakudoh, Takashi; Kawasaki, Hideki; Iwanaga, Masako; Araki, Kyosuke; Fujimoto, Haruhiro; Takada, Naoko; Iwano, Hidetoshi; Tsuchida, Kozo

doi:10.1016/j.jinsphys.2009.08.001

Cited by 17 publications

(23 citation statements)

References 24 publications

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“…The primer sequences have been described previously [19][20][21] and are listed in Supplementary Table S1 (http://www.biochemj.org/bj/459/bj4590137add.htm). The primer sequences have been described previously [19][20][21] and are listed in Supplementary Table S1 (http://www.biochemj.org/bj/459/bj4590137add.htm).…”

Section: Qpcr (Quantitative Real-time Pcr) Analysismentioning

confidence: 99%

Niemann–Pick disease type C2 protein induces triglyceride accumulation in silkworm and mammalian cell lines

Adachi

Ishii

Matsumoto

et al. 2014

Biochemical Journal

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Silkworm haemolymph induced both the cessation of growth and an increase in triglyceride (triacylglycerol) storage in BmN4 cells. We purified the growth inhibitory factor from the silkworm haemolymph and identified this protein as the Bombyx mori PP (promoting protein), an orthologue of NPC2 (Niemann-Pick disease type C2) protein. Recombinant silkworm NPC2 inhibited cellular proliferation and increased triglyceride accumulation in BmN4 cells. Injection of either the recombinant protein or antiserum of NPC2 into living silkworms increased or decreased respectively triglyceride levels in the fat body. A mutation that depletes the cholesterol-binding capacity did not abolish the activity of NPC2. We further revealed that NPC2 induced the phosphorylation of AMPK (AMP-activated protein kinase) and that an AMPK inhibitor suppressed NPC2-dependent triglyceride accumulation. These findings suggest that NPC2 induces triglyceride accumulation via the activation of AMPK independently of its cholesterol-binding capacity in the silkworm.

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Section: Qpcr (Quantitative Real-time Pcr) Analysismentioning

confidence: 99%

Niemann–Pick disease type C2 protein induces triglyceride accumulation in silkworm and mammalian cell lines

Adachi

Ishii

Matsumoto

et al. 2014

Biochemical Journal

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“…Quantitative PCR (quantitative RT-PCR: qPCR) was performed and analyzed as described previously (Wang et al, 2009). qPCR was conducted on an ABI7500 real-time PCR machine (Applied Biosystems) using FastStart Universal SYBR Green Master (Roche).…”

Section: Quantitative Pcrmentioning

confidence: 99%

20-hydroxyecdysone and juvenile hormone analog prevent precocious metamorphosis in recessive trimolter mutants of Bombyx mori

Wang

Ali

Moriyama

et al. 2012

Insect Biochemistry and Molecular Biology

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“…: BAC67246), and BmIDGF (Imaginai disc growth factor, GenBank accession No. : NP_001036847) were previously reported (Kim et al 1998; Daimon et al 2003; Wang et al 2009). These three proteins were renamed based on the phylogenetic analysis of the catalytic domain.…”

Section: Resultsmentioning

confidence: 86%

“…In Mamestra brassicae , recombinant M. brassicae IDGF could stimulate the growth of cells derived from the fat body and hemocytes without insulin (Zhang et al 2006). Previous work (Wang et al 2009; Pan et al 2010) found that BmIDGF was expressed in all developmental stages of silkworm larvae and various larval tissues and was located in the extracellular space. It was predicted that BmIDGF is produced mainly by the fat body, secreted into the hemolymph, and then brought to other organs.…”

Section: Resultsmentioning

confidence: 93%

In silicoIdentification of Novel Chitinase-Like Proteins in the Silkworm,Bombyx mori, Genome

Pan

Wang

et al. 2012

Journal of Insect Science

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In insects, chitinases participate in the periodic shedding of old exoskeletons and the turnover of peritrophic membranes. Chitinase family members have been identified in dozens of species, including Tribolium castaneum, Drosophila melanogaster, and Anopheles gambiae. In this study, nine chitinases and three hypothetical chitinases have been identified in Bombyx mori L. (Lepidoptera: Bombycidae) through genome-wide searching. Phylogenetic analyses revealed that seven of them belong to the seven chitinase groups, respectively. BmCht25 and BmCht26 could not be grouped into the known chitinase groups, and might belong to two new groups of the chitinase family. BmCht10, BmCht25, and BmIDGF have glutamate amino acid substitutions in the active catalytic domain. Only BmCht5 and BmCht10 contain CBD domain and PEST sequences (rich in proline, glutamic acid, serine, and threonine). BmCht5 and BmCht26 are located on chromosome 7, and others (BmCht6, BmCht7, BmCht10, BmCht11, BmCht20, BmIDGF) are located on separate chromosomes of Bombyx mori, respectively. The present study provides important background information for future studies using Bombyx mori as a model organism for insect development and virus and host interaction.

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Purification and expression analysis of imaginal disc growth factor in the silkworm, Bombyx mori

Cited by 17 publications

References 24 publications

Niemann–Pick disease type C2 protein induces triglyceride accumulation in silkworm and mammalian cell lines

Niemann–Pick disease type C2 protein induces triglyceride accumulation in silkworm and mammalian cell lines

20-hydroxyecdysone and juvenile hormone analog prevent precocious metamorphosis in recessive trimolter mutants of Bombyx mori

In silicoIdentification of Novel Chitinase-Like Proteins in the Silkworm,Bombyx mori, Genome

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