Purification and fractionation of phycobiliproteins from Arthrospira platensis and Corallina officinalis with evaluating their biological activities

Abstract

Phycobiliproteins (PBPs) are a class of water-soluble pigments with a variety of biological functions that are present in red macroalgae and cyanobacterial species. The crude forms of phycocyanin (C-PC) from the blue green alga Arthrospira platensis and allophycocyanin (APC) from the red macroalga Corallina officinalis were extracted and purified by ammonium sulphate precipitation, anion exchange chromatography, and size exclusion chromatography methods, respectively. The obtained C-PC and APC from A. platensis and C. officinalis were 0.31 mg/mL and 0.08 mg/mL, respectively, with molecular masses of “17.0 KDa and 19.0 KDa” and “15.0 KDa and 17.0 KDa” corresponding to α and β subunits, respectively. FT-IR was used to characterize the purified APC and C-PC in order to look into their structures. Highly purified extracts (A620/A280 > 4.0) were obtained from subtractions’ PC3 and PC4 that were tested for their biological activities. APC and C-PC crude extracts plus their fractions exhibited potent anti-oxidant in different ratios by using three techniques. PC1 showed high anti-inflammatory (75.99 and 74.55%) and anti-arthritic (78.89 and 76.92%) activities for C. officinalis and A. platensis, respectively compared with standard drugs (72.02 and 71.5%). The methanolic and water extracts of both species showed greater antibacterial efficacy against Gram +ve than Gram −ve marine bacteria. Our study shed light on the potential medical uses of C-PC and APC extracted from the tested species as natural substances in a variety of foods and drugs. Further investigations are required to explore the diverse chemical natures of distinct PBPs from different cyanobacteria and red algae because their amino acid sequences vary among different algal species.

Incidence, mortality, and predictors of acute kidney injury in patients with heart failure: a systematic review

“…The incidence of AKI in patients with HF has been shown to be as high as 27–45%, 44 , 45 , 46 which is substantially higher than the incidence of AKI in hospitalized general patients (3.2–9.6%), 47 showing that HF may increase the incidence of AKI in hospitalized patients. Our systematic review and meta‐analysis involved 37 studies, and we found a pooled incidence of AKI of 33% in patients with HF.…”

Influence of Critical Parameters on the Extraction of Concentrated C-PE from Thermotolerant Cyanobacteria

“…Although statistical tools were used, such as in the design of experiments, the response surface for purity shows that, unlike the C-PE concentration, its purity is far from presenting results that can be considered optimal. It should be noted that the test results show that adjusting the extraction conditions makes it possible to obtain an extract with a considerable concentration of extracted C-PE and a purity of higher than 0.7 (considered as food-grade) [ 51 , 52 ]. At the same time, it is assumed that the conditions are to bring out as much protein as possible.…”