Purification and further characterization of a glycoprotein from the skin mucus of Japanese eels

Sumi, Toshihisa; Hama, Yoichiro; Nakagawa, Hiroki; Maruyama, Daisuke; Asakawa, Makio

doi:10.1111/j.1095-8649.2004.00286.x

Cited by 5 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Atlantic salmon, sialyl Tn was the predominant structure in skin mucins, whereas sialyl core 5 [GalNAcα1-3(NeuAcα2-6)GalNAcol] was predominant on GI mucins. Core 5-containing O -glycans were predominant structures of Atlantic salmon skin and GI mucins, which is in agreement with previous studies on rainbow trout skin, , egg envelope, and Japanese eel skin (Anguilla japonica) . The GI predominant mucin O -glycan core structures in Atlantic salmon are different from the human ones.…”

Section: Discussionsupporting

confidence: 90%

“…Core 5containing O-glycans were predominant structures of Atlantic salmon skin and GI mucins, which is in agreement with previous studies on rainbow trout skin, 16,17 egg envelope, 26 and Japanese eel skin (Anguilla japonica). 28 The GI predominant mucin O-glycan core structures in Atlantic salmon are different from the human ones. In humans, core 3 is the predominant structure, although both cores 3 and 5 are present on mucins isolated from the GI tract.…”

Section: ■ Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Atlantic Salmon Carries a Range of Novel O-Glycan Structures Differentially Localized on Skin and Intestinal Mucins

et al. 2015

View full text Add to dashboard Cite

Aquaculture is a growing industry, increasing the need for understanding host−pathogen interactions in fish. The skin and mucosal surfaces, covered by a mucus layer composed of mucins, is the first point of contact between fish and pathogens. Highly O-glycosylated mucins have been shown to be an important part of the defense against pathogens, and pathogens bind to host surfaces using lectinlike adhesins. However, knowledge of piscine O-glycosylation is very limited. We characterized mucin O-glycosylation of five freshwater acclimated Atlantic salmon, using mass spectrometry. Of the 109 O-glycans found, most were sialylated and differed in distribution among skin, pyloric ceca, and proximal and distal intestine. Skin O-glycans were shorter (2−6 residues) and less diverse (33 structures) than intestinal O-glycans (2−13 residues, 93 structures). Skin mucins carried Oglycan cores 1, 2, 3, and 5 and three types of sialic acids (Neu5Ac, Neu5Gc, and Kdn) and had sialyl-Tn as the predominant structure. Intestinal mucins carried only cores 1, 2, and 5, Neu5Ac was the only sialic acid present, and sialylated core 5 was the most dominant structure. This structural characterization can be used for identifying structures of putative importance in host− pathogen interactions for further testing in biological assays and disease intervention therapies.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: ■ Discussionmentioning

confidence: 99%

Atlantic Salmon Carries a Range of Novel O-Glycan Structures Differentially Localized on Skin and Intestinal Mucins

et al. 2015

View full text Add to dashboard Cite

show abstract

“…From the different proteins present in mucus surrounding epithelial tissues, mucin is one of the most conserved and abundant (Sumi et al ., 1997). In fact, the gill mucin from eels has been chemically characterized as a glycoprotein, similar to other mammalian mucins (Sumi et al ., 2004). When mammalian mucin was tested as substratum in adhesion and chemotaxis assays, the results were statistically similar to those found with gill mucus.…”

Section: Discussionmentioning

confidence: 99%

Role of the metalloprotease Vvp and the virulence plasmid pR99 of Vibrio vulnificus serovar E in surface colonization and fish virulence

Valiente¹,

Lee²,

Hor³

et al. 2007

Environmental Microbiology

View full text Add to dashboard Cite

The virulence for eels of Vibrio vulnificus biotype 2 serovar E (VSE) is conferred by a plasmid that codifies ability to survive in eel serum and cause septicaemia. To find out whether the plasmid and the selected chromosomal gene vvp plays a role in the initial steps of infection, the VSE strain CECT4999, the cured strain CT218 and the Vvp-deficient mutant CT201 (obtained in this work by allelic exchange) were used in colonization and virulence experiments. The eel avirulent biotype 1 (BT1) strain YJ016, whose genome has been sequenced, was used for comparative purposes. The global results demonstrate that the plasmid does not play a significant role in surface colonization because (i) CECT4999 and CT218 were equally chemoattracted towards and adherent to eel mucus and gills, and (ii) CT218 persisted in gills from bath-infected eels 2 weeks post infection. In contrast, mutation in vvp gene reduced significantly chemoattraction and attachment to eel mucus and gills, as well as virulence degree by immersion challenge. Co-infection experiments by bath with CECT4999 and CT201 confirmed that Vvp was involved in eel colonization and persistence in gills, because CECT4999 was recovered at higher numbers compared with CT201 from both internal organs of moribund fish (ratio 4:1) and gills from survivors (ratio 50:1). Interestingly, YJ016 also showed chemoattraction and attachment to mucus, and complementation of CT201 with BT1-vvp gene restored both activities together with virulence degree by immersion challenge. Additional experiments with algae mucus and purified mucin gave similar results. In conclusion, the protease Vvp of V. vulnificus seems to play an essential role in colonization of mucosal surfaces present in aquatic environments. Among the V. vulnificus strains colonizing fish mucus, only those harbouring the plasmid could survive in blood and cause septicaemia.

show abstract

Mucin from loach skin mucus and its interfacial behavior on gold surface

Wang

Song

et al. 2014

Chin J Polym Sci

View full text Add to dashboard Cite

Purification and further characterization of a glycoprotein from the skin mucus of Japanese eels

Cited by 5 publications

References 22 publications

Atlantic Salmon Carries a Range of Novel O-Glycan Structures Differentially Localized on Skin and Intestinal Mucins

Atlantic Salmon Carries a Range of Novel O-Glycan Structures Differentially Localized on Skin and Intestinal Mucins

Role of the metalloprotease Vvp and the virulence plasmid pR99 of Vibrio vulnificus serovar E in surface colonization and fish virulence

Mucin from loach skin mucus and its interfacial behavior on gold surface

Contact Info

Product

Resources

About