Purification and identification of a novel cutinase from Coprinopsis cinerea by adsorptive bubble separation

Foaming of proteins: New prospects for enzyme purification processes

“…Electrostatic repul- sive forces between the adsorbed molecules are at a minimum. Merz et al (2009) obtained maximal enzyme recovery of a cutinase close to its pI, and Loha et al (1997) observed an optimized transport of invertase and alpha-amylase both at their isoelectric points.…”

“…Not much attention is paid to the actual enzyme concentrations and the matrices of the media generated during the fermentation processes. Foaming of multi-component culture media was performed rather rarely (Holmström, 1968;Fernandes et al, 2002a;Linke et al, 2005aLinke et al, , 2007Linke et al, , 2009Merz et al, 2009;Sarkar et al, 1987). A crude pellet culture of a basidiomycete was submitted to the fractionation process resulting in equivalent recovery, enrichment and purification in the cell free foam compared to the filtrated supernatant (Linke et al, 2007).…”

Characterization of an acidic cold-adapted cutinase from Thielavia terrestris and its application in flavor ester synthesis

“…Cutinases are mainly found in different species of fungi (Castro-Ochoa et al, 2012;Fraga, Carvalho, & Macedo, 2012;Nyyssölä et al, 2014;Pio & Macedo, 2007;Roussel et al, 2014;Speranza & Macedo, 2013), although several bacterial cutinolytic enzymes have also been reported (Dutta, Krishnamoorthy, & Dasu, 2013;Hegde & Veeranki, 2013;Kitadokoro et al, 2012). A number of cutinases have been purified and characterized from various fungi, including Alternaria brassicicola (Koschorreck, Liu, Kazenwadel, Schmid, & Hauer, 2010), Coprinopsis cinerea (Merz, Schembecker, Riemer, Nimtz, & Zorn, 2009), Fusarium oxysporum (Fraga et al, 2012;Speranza & Macedo, 2013), Fusarium solani (Kwon, Kim, Yang, Song, & Song, 2009), Monilinia fructicola (Wang, Michailides, Hammock, Lee, & Bostock, 2002), Sirococcus conigenus , Trichoderma harzianum (Rubio, Cardoza, Hermosa, Gutierrez, & Monte, 2008) and Trichoderma reesei (Roussel et al, 2014). Most of these are mesophilic fungi; there are no reports on cutinases from thermophilic fungi, except for Humicola insolens (Nielsen, Borch, & Westh, 2007).…”

Recovery of Extracellular Lipolytic Enzymes fromMacrophomina phaseolinaby Foam Fractionation with Air

“…However, it has been demonstrated that larger bubbles increase enrichment [30, 31]. Some works, which start from bulk solutions of known protein/enzyme content, show the influence of gas flow rate on enrichment and recovery during batch foaming [32, 33]. No reports were found showing the influence on the efficiency parameters of varying the gas flow rate during a batch foam separation.…”

“…Besides, when a surfactant was used to help foam fractionate enzymes, the majority of experiments report the addition of a nonnegligible amount of the substance to the initial solution [21, 33, 34], which could render the purified enzymes suitable for restricted applications [35]. Some reports even show that the use of a surfactant denatured the enzyme [28].…”