Purification and identification of an angiotensin I-converting enzyme inhibitory peptide from fermented soybean extract

In vitro and in vivo antihypertensive activity of palm kernel cake protein hydrolysates: Sequencing and characterization of potent bioactive peptides

“…Numerous studies on alleviating hypertension have focused on the generation and isolation of ACE-inhibitory compounds from various food sources (Boschin et al, 2014;Lin et al, 2011;Rho et al, 2009;Wu et al, 2008). Among the natural sources of ACE inhibitors, plant protein hydrolysate and bio-peptides are among the promising alternatives to synthetic drugs.…”

Bioactive peptides from meat and their influence on human health

“…For the same reason, hydrophilic peptides are incompatible with the active sites of ACE, and exhibit none or a weak ACE inhibitory activity (Li et al, 2004;Matsui and Matsumoto, 2006;Ryan et al, 2011). Studies found that N-terminal end of the peptides with ACE-inhibitory activity is hydrophobic (Hayes et al, 2007;Rho et al, 2009;Ryan et al, 2011). Moreover, it has been found that amino acid at the position three from the C-terminal requires the L-configuration (Hernández-Ledesma et al, 2011).…”

Concentration and selective fractionation of an antihypertensive peptide from an alfalfa white proteins hydrolysate by mixed ion-exchange centrifugal partition chromatography

“…Nevertheless, these hydrolysates are usually composed of about one hundred different peptides and therefore, an enrichment of raw mixtures in bioactive peptides must be envisaged in order to increase the target biological activity. In most cases, the enrichment is achieved by a tangential ultrafiltration step combined to at least two low-pressure liquid chromatographic steps (ion-exchange chromatography then size exclusion chromatography) [4][5][6]. The purification factor observed after such a process combination classically ranges from 10 to 15 and is costly and time consuming.…”