Purification and Identification of Antioxidant Peptides from Protein Hydrolysate of Scalloped Hammerhead (Sphyrna lewini) Cartilage

Li, Xuerong; Chi, Chang-Feng; Li, Li; Wang, Bin

doi:10.3390/md15030061

Cited by 55 publications

(39 citation statements)

References 51 publications

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“…Though, the DH reported for goby Figure 5: Reductive Potential of Mormyrus rume Muscle Protein Hydrolysates muscle protein using crude enzymes from different sources (Nasri et al, 2014) was lower compared to the present result, the shape of hydrolysis curves is similar. The degree of hydrolysis reported in the present work compared well with the DH published by Li et al (2017) for scalloped hammerhead (Sphyrna lewini) cartilage which ranges from 18.33% to 23.72%. In contrast to the above reports, higher DH (76.29% and 63.49%) was published for red tilapia (Oreochromis niloticus) byproduct protein using thermolysin and alcalase for hydrolysis, respectively (Roslan et al, 2014).…”

Section: Discussionsupporting

confidence: 86%

Comparative studies on the antioxidant potential of hydrolysates of <i>Mormyrus rume</i> muscle protein

Jerome¹,

Odekanyin²

2019

Ife Journal of Science

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This study determined the degree of hydrolysis of Mormyrus rume muscle proteins by digestive proteases and the antioxidant potential of the hydrolysates produced was evaluated. The proteins were extracted with distilled water adjusted to neutral pH. The proteases used include trypsin, chymotrypsin and pepsin and the degree of hydrolysis was determined using trichloroacetic acid precipitation method. The antioxidative activities of the hydrolysates were evaluated using different in-vitro antioxidant assay methods such as 1,1-diphenyl-2picrylhydrazyl (DPPH) radical scavenging, hydrogen peroxide scavenging, metal chelating capacity and reductive potentials. Degree of hydrolysis was generally low and ranges between 17.68% and 32.20% with pepsin given the highest degree of hydrolysis. The protein hydrolysates obtained with trypsin exhibited the highest antioxidant activities when assessed with metal chelating activity assay with half maximal inhibitory concentration (IC ) of 0.32 ± 0.01 mg/ml; hydrogen peroxide scavenging assay with IC of 0.18 ± 0.01 mg/ml 50 50and reductive potential of 0.41 ± 0.002 units at the highest concentration tested (1.0 mg/ml). Pepsin-produced hydrolysates showed the highest DPPH radical scavenging potential at all concentrations with IC of 0.35 ± 50 0.003 mg/ml. The study concluded that the hydrolysates produced contain bioactive peptides which possessed significant amount of antioxidant activities, which may be a promising health promoting agent if isolated.ABSTRACT 431

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Section: Discussionsupporting

confidence: 86%

Comparative studies on the antioxidant potential of hydrolysates of <i>Mormyrus rume</i> muscle protein

Jerome¹,

Odekanyin²

2019

Ife Journal of Science

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“…Low-MW antioxidant peptides have a highly pronounced ability to hold back the oxidative stress because they can easily interact with target radicals to terminate the free radical chain reactions [5,49,50]. In addition, Li et al reported that the antioxidant activities of protein hydrolysates with average MW (AMW) from 0.64 kDa to 257.19 kDa were negatively related to the logarithm of their AMW [31].…”

Section: Structure-activity Relationship Of Mmp-4 Mmp-7 and Mmp-12mentioning

confidence: 99%

Antioxidant Peptides from the Protein Hydrolysate of Monkfish (Lophius litulon) Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by H2O2

Wang

Zhao

et al. 2020

Marine Drugs

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In the work, defatted muscle proteins of monkfish (Lophius litulon) were separately hydrolyzed by pepsin, trypsin, and in vitro gastrointestinal (GI) digestion methods, and antioxidant peptides were isolated from proteins hydrolysate of monkfish muscle using ultrafiltration and chromatography processes. The antioxidant activities of isolated peptides were evaluated using radical scavenging and lipid peroxidation assays and H2O2-induced model of HepG2 cells. In which, the cell viability, reactive oxygen species (ROS) content, and antioxidant enzymes and malondialdehyde (MDA) levels were measured for evaluating the protective extent on HepG2 cells damaged by H2O2. The results indicated that the hydrolysate (MPTH) prepared using in vitro GI digestion method showed the highest degree of hydrolysis (27.24 ± 1.57%) and scavenging activity on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (44.54 ± 3.12%) and hydroxyl radical (41.32 ± 2.73%) at the concentration of 5 mg protein/mL among the three hydrolysates. Subsequently, thirteen antioxidant peptides (MMP-1 to MMP-13) were isolated from MPTH. According to their DPPH radical and hydroxyl radical scavenging activity, three peptides with the highest antioxidant activity were selected and identified as EDIVCW (MMP-4), MEPVW (MMP-7), and YWDAW (MMP-12) with molecular weights of 763.82, 660.75, and 739.75 Da, respectively. EDIVCW, MEPVW, and YWDAW showed high scavenging activities on DPPH radical (EC50 0.39, 0.62, and 0.51 mg/mL, respectively), hydroxyl radical (EC50 0.61, 0.38, and 0.32 mg/mL, respectively), and superoxide anion radical (EC50 0.76, 0.94, 0.48 mg/mL, respectively). EDIVCW and YWDAW showed equivalent inhibiting ability on lipid peroxidation with glutathione in the linoleic acid model system. Moreover, EDIVCW, MEPVW, and YWDAW had no cytotoxicity to HepG2 cells at the concentration of 100.0 µM and could concentration-dependently protect HepG2 cells from H2O2-induced oxidative damage through decreasing the levels of reactive oxygen species (ROS) and MDA and activating intracellular antioxidant enzymes of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). These present results indicated that the protein hydrolysate and isolated antioxidant peptides from monkfish muscle, especially YWDAW could serve as powerful antioxidants applied in the treatment of some liver diseases and healthcare products associated with oxidative stress.

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“…For in vitro systems where no chemical oxidizing agent is added, but LPO occurs through different pathways, lipid peroxides thus formed become the main oxidizing compounds in the medium, and LPO can be quantified by using a reagent prone to be oxidized by lipid peroxides (amount of reagent lost can be converted in amount of lipid peroxides). This is the case of the thiocyanate method [68], which has been used to track LPO in lipid-solution-based LPIP assays (as described in the next section) [69][70][71][72][73][74][75][76][77][78]. Given the lack of an oxidizing trigger in these assays, the capacity of the medium to oxidize iron (II) to iron (III) is directly proportional to the concentration of lipid peroxides.…”

Section: On the Quantification Of Lipid Peroxidationmentioning

confidence: 99%

“…Importantly, one should note that quantifying the lipid peroxides of a sample is a measure of LPO that will decay along time, as lipid peroxides degrade into smaller molecules; thus, it is a measure of "early lipid peroxidation". In the thiocyanate method, ferrous ions are oxidized into ferric ions, which in turn form a colored complex with thiocyanate (ferric thiocyanate), with maximum absorbance at 485 nm [68][69][70][75][76][77].…”

Section: On the Quantification Of Lipid Peroxidationmentioning

confidence: 99%

“…Thus, lipid peroxidation can be detected by using methods that rely on oxidation themselves, since the lipid peroxides will act on the reagents and cause their oxidative state to increase, developing color. For this reason, linoleic-acid-based systems have often been used as LPO quantification method in the thiocyanate-iron(III) complex spectrophotometric assay [69][70][71][72][73][74][75][76][77][78], which is an advantage of using this substrate given the increased simplicity and decreased interferences of this method.…”

Section: Watermentioning

confidence: 99%

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Evaluating the In Vitro Potential of Natural Extracts to Protect Lipids from Oxidative Damage

et al. 2020

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Lipid peroxidation is a chemical reaction known to have negative impacts on living organisms’ health and on consumer products’ quality and safety. Therefore, it has been the subject of extensive scientific research concerning the possibilities to reduce it, both in vivo and in nonliving organic matrices. It can be started by a variety of oxidants, by both ROS-dependent and -independent pathways, all of them reviewed in this document. Another feature of this reaction is the capacity of lipid peroxyl radicals to react with the non-oxidized lipids, propagating the reaction even in the absence of an external trigger. Due to these specificities of lipid peroxidation, regular antioxidant strategies—although being helpful in controlling oxidative triggers—are not tailored to tackle this challenge. Thus, more suited antioxidant compounds or technologies are required and sought after by researchers, either in the fields of medicine and physiology, or in product development and biotechnology. Despite the existence of several laboratory procedures associated with the study of lipid peroxidation, a methodology to perform bioprospecting of natural products to prevent lipid peroxidation (a Lipid Peroxidation Inhibitory Potential assay, LPIP) is not yet well established. In this review, a critical look into the possibility of testing the capacity of natural products to inhibit lipid peroxidation is presented. In vitro systems used to peroxidize a lipid sample are also reviewed on the basis of lipid substrate origin, and, for each of them, procedural insights, oxidation initiation strategies, and lipid peroxidation extent monitoring are discussed.

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Purification and Identification of Antioxidant Peptides from Protein Hydrolysate of Scalloped Hammerhead (Sphyrna lewini) Cartilage

Cited by 55 publications

References 51 publications

Comparative studies on the antioxidant potential of hydrolysates of <i>Mormyrus rume</i> muscle protein

Comparative studies on the antioxidant potential of hydrolysates of <i>Mormyrus rume</i> muscle protein

Antioxidant Peptides from the Protein Hydrolysate of Monkfish (Lophius litulon) Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by H2O2

Evaluating the In Vitro Potential of Natural Extracts to Protect Lipids from Oxidative Damage

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