Purification and Identification of Flavonoids from the Yellow Green Cocoon Shell (Sasamayu) of the Silkworm,Bombyx mori

Kurioka, Akira; Yamazaki, Motoyoshi

doi:10.1271/bbb.66.1396

Cited by 85 publications

(61 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Yellow-, pink-, golden-yellow-, and flesh-colored cocoon pigments are derivatives of carotenoids (9), whereas sasaand green-cocoon pigments are derivatives of flavonoids (10,11). These pigments protect pupae from sunlight as antioxidants (12).…”

Section: The Human Macular Carotenoid Transporter Discovered Using Anmentioning

confidence: 99%

Can the silkworm (Bombyx mori) be used as a human disease model?

Tabunoki

Bono

Ito

et al. 2016

DD&T

View full text Add to dashboard Cite

Section: The Human Macular Carotenoid Transporter Discovered Using Anmentioning

confidence: 99%

Can the silkworm (Bombyx mori) be used as a human disease model?

Tabunoki

Bono

Ito

et al. 2016

DD&T

View full text Add to dashboard Cite

“…The pigments in yellow, pink, golden-yellow and flesh cocoons are derived from carotenoids [1], while those of sasa and green are from flavonoids [2,3]. These pigments are absorbed from mulberry leaves, transferred from the midgut to the silk gland via the hemolymph, and are accumulated in the layers of the cocoon sericine [4].…”

Section: Introductionmentioning

confidence: 99%

A carotenoid‐binding protein (CBP) plays a crucial role in cocoon pigmentation of silkworm (Bombyx mori) larvae

et al. 2004

View full text Add to dashboard Cite

We examined the role of carotenoid-binding protein (CBP) in yellow cocoon pigmentation. First, using yellow or white cocoon races, we investigated the linkage between the yellow pigmentation and CBP expression. CBP was expressed only in the silk gland of the yellow cocoon races, which utilize carotenoids for cocoon pigmentation. Furthermore, CBP expression in the silk glands of day 1-7 fifth instar larvae matched the period of carotenoid uptake into the silk gland. Finally, we gave double-stranded CBP RNA to Bombyx mori (B. mori) larvae to induce RNA interference. The significantly reduced expression of CBP in the silk gland of fifth instar larva was confirmed on day 4 and a decrease in yellow pigmentation was observed in the cocoon. We showed that CBP plays a key role in the yellow cocoon pigmentation caused by carotenoids.

show abstract

“…This is mainly because the metabolic processing of flavonoids absorbed from diets is much more complex than that of carotenoids. In fact, green cocoons of some silkworm varieties contain more than 30 kinds of flavonoids that are not present in their diet (8)(9)(10)(11)(12), whereas the main constituents of yellow and pinkish cocoons are lutein and β-carotene (5, 7), respectively, both of which are original components in mulberry leaves. In addition, green cocoon is usually observed as a quantitative trait (i.e., continuous color variations ranging from pale or light green to deep or yellowish green), modified variously by environmental conditions, and governed by many loci (2,3,5).…”

mentioning

confidence: 99%

The silkworm Green b locus encodes a quercetin 5- O -glucosyltransferase that produces green cocoons with UV-shielding properties

Daimon

Hirayama

Kanai

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

106

View full text Add to dashboard Cite

In the silkworm Bombyx mori, dietary flavonoids are metabolized and accumulate in cocoons, thereby causing green coloration. Classical genetic studies suggest that more than seven independent loci are associated with this trait; however, because of the complex inheritance pattern, none of these loci have been characterized molecularly, and a plausible and comprehensive model for their action has not been proposed. Here, we report the identification of the gene responsible for the Green b (Gb) locus involving the green cocoon trait. In + Gb animals, glucosylation at the 5-O position of dietary quercetin did not occur, and the total amount of flavonoids in tissues and cocoons was dramatically reduced. We performed positional cloning of Gb and found a 38-kb deletion in a UDP-glucosyltransferase (UGT) gene cluster associated with the + Gb allele. RT-PCR and biochemical studies suggested that deletion of Bm-UGT10286 (UGT) is responsible for Gb and Bm-UGT10286 is virtually the sole source of UGT activity toward the 5-O position of quercetin. Our data show that the regiospecific glucosylation of flavonoids by the quercetin 5-O-glucosyltransferase can greatly affect the overall bioavailability of flavonoids in animals. Furthermore, we provide evidence that flavonoids increase the UV-shielding activity of cocoons and thus could confer an increased survival advantage to insects contained in these cocoons. This study will lead to greater understanding of mechanisms for metabolism, uptake, and transport of dietary flavonoids, which have a variety of biological activities in animals and beneficial effects on human health.

show abstract

Purification and Identification of Flavonoids from the Yellow Green Cocoon Shell (Sasamayu) of the Silkworm,Bombyx mori

Cited by 85 publications

References 10 publications

Can the silkworm (<i>Bombyx mori</i>) be used as a human disease model?

Can the silkworm (<i>Bombyx mori</i>) be used as a human disease model?

A carotenoid‐binding protein (CBP) plays a crucial role in cocoon pigmentation of silkworm (Bombyx mori) larvae

The silkworm Green b locus encodes a quercetin 5- O -glucosyltransferase that produces green cocoons with UV-shielding properties

Contact Info

Product

Resources

About