Purification and identification of globulin-1 S allele as a novel allergen with N-glycans in wheat (Triticum aestivum)

Zhu, Chengbo; Wang, Chong; Zhou, Jinru; Li, Han; Chen, Qing; Fu, Linglin

doi:10.1016/j.foodchem.2022.133189

Cited by 7 publications

(1 citation statement)

References 30 publications

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“…These results are in agreement with a previous study which reported that major oat globulins had molecular weight between ∼53 and 62 kDa . The structure of the wheat globulin-1 S allele has been characterized in a recent study which reported that the globulin-1 S allele is one of the most abundant glycoproteins in wheat and has a potential allergenicity due to its IgE-binding capacity . Future studies are needed to assess the quantity of the globulin-1 S allele in the OPI, which could be a potential allergen.…”

Section: Resultssupporting

confidence: 93%

Determining the Impact of Genotype × Environment on Oat Protein Isolate Composition Using HPLC and LC-MS Techniques

Mel,

Rampitsch,

Zvomuya

et al. 2024

J. Agric. Food Chem.

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The effect of genotype and environment on oat protein composition was analyzed through size exclusion-highperformance liquid chromatography (SE-HPLC) and liquid chromatography-mass spectrometry (LC-MS) to characterize oat protein isolate (OPI) extracted from three genotypes grown at three locations in the Canadian Prairies. SE-HPLC identified four fractions in OPI, including polymeric globulins, avenins, glutelins, and albumins, and smaller proteins. The protein composition was dependent on the environment, rather than the genotype. The proteins identified through LC-MS were grouped into eight categories, including globulins, prolamins/avenins, glutelins, enzymes/albumins, enzyme inhibitors, heat shock proteins, grain softness proteins, and allergenic proteins. Three main globulin protein types were also identified, including the P14812|SSG2-12S seed storage globulin, the Q6UJY8_TRITU-globulin, and the M7ZQM3_TRIUA-Globulin-1 S. Principal component analysis indicated that samples from Manitoba showed a positive association with the M7ZQM3_TRIUA-Globulin-1 S allele and Q6UJY8_TRITU-globulin, while samples from Alberta and Saskatchewan had a negative association with them. The results show that the influence of G × E on oat protein fractions and their relative composition is crucial to understanding genotypes' behavior in response to different environments.

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Section: Resultssupporting

confidence: 93%