1986
DOI: 10.1016/s0021-9258(18)66730-4
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Purification and identification of the functional sodium- and chloride-coupled gamma-aminobutyric acid transport glycoprotein from rat brain.

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Cited by 178 publications
(51 citation statements)
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“…As can be seen in Figure 3A-where the same experiment was done as in Figure 1 except that the proteoliposomes were prepared in a potassium-containing rather than a sodium-containing medium-there is a GABAdependent of 22Na+ uptake. GABA influx is an electrogenic process that is strongly stimulated by an interior negative membrane potential (Kanner, 1978;Radian & Kanner, 1985; Radian et al, 1986). Addition of valinomycin to the potassium-containing proteoliposomes not only strongly stimulates GABA influx, its presence is required to detect the GABAdependent sodium flux (Table I).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…As can be seen in Figure 3A-where the same experiment was done as in Figure 1 except that the proteoliposomes were prepared in a potassium-containing rather than a sodium-containing medium-there is a GABAdependent of 22Na+ uptake. GABA influx is an electrogenic process that is strongly stimulated by an interior negative membrane potential (Kanner, 1978;Radian & Kanner, 1985; Radian et al, 1986). Addition of valinomycin to the potassium-containing proteoliposomes not only strongly stimulates GABA influx, its presence is required to detect the GABAdependent sodium flux (Table I).…”
Section: Resultsmentioning
confidence: 99%
“…This might constitute a cellular mechanism by which the transporter could be regulated; conversion of the high-affinity to the low-affinity form might very effectively stop reuptake of GABA from the synaptic cleft. The high-affinity GABA transport has been purified to homogeneity in a reconstitutively active form, and antibodies against it have been prepared (Radian et al, 1986).…”
Section: Discussionmentioning
confidence: 99%
“…Despite the challenges, the use of reconstitution of transport activity to monitor the purification led to the successful isolation of a GABA transporter [ 14 , 20 ] by Rodica Radian-Gordon and Annie Bendahan in Kanner’s laboratory (Fig. 6 a and b).…”
Section: Scientific Impact Of This Reconstitution Methodsmentioning
confidence: 99%
“…She worked with Kanner for 30 years from 1981 and generated or helped generating many of the most important data from the laboratory. b Rodica Radian-Gordon who isolated GABA transporter 1 [ 14 , 20 ] is here depicted in the laboratory assisted by her husband, Alexander Gordon (Alex). Her career path has been extraordinary.…”
Section: Scientific Impact Of This Reconstitution Methodsmentioning
confidence: 99%
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