Purification and Immobilization of Superoxide Dismutase Obtained from Saccharomyces cerevisiae TBRC657 on Bacterial Cellulose and its Protective Effect against Oxidative Damage in Fibroblasts

Pinmanee, Phitsanu; Sompinit, Kamonwan; Jantimaporn, Angkana; Khongkow, Mattaka; Haltrich, Dietmar; Nimchua, Thidarat; Sukyai, Prakit

doi:10.3390/biom13071156

Cited by 9 publications

(3 citation statements)

References 47 publications

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“…Kefir grain bacteria and yeast are thought to be the sources of antioxidants in WJB kefir. One of these antioxidants, superoxide dismutase (SOD), comes from the cellular antioxidant system ( Najmuldeen et al, 2019 ; Pinmanee et al, 2023 ). Superoxide is one of the most abundant reactive oxygen species (ROS) produced by the mitochondria, while SOD catalyzes the breakdown of superoxide into hydrogen peroxide and water and is therefore a central regulator of ROS levels ( Najmuldeen et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Potential of White Jack Bean (Canavalia ensiformis L. DC) Kefir as a Microencapsulated Antioxidant

Yusuf,

Kholifaturrohmah,

Nurcholis

et al. 2023

Prev. Nutr. Food Sci.

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Oxidative stress plays a major role in the pathogenesis and progression of noncommunicable diseases. Kefir is a fermented food that has been reported to repress oxidative stress. This study aimed to assess the antioxidant activity, bioactive composition, and encapsulation efficiency of white jack bean (WJB) kefir. The following procedures were conducted: WJB was prepared and converted into juice using water solvent. The sterilized WJB juice was then fermented with kefir grain (10%) for 24∼72 h. Every 24 h, the kefir was evaluated for antioxidant activity, and the dominant bioactive component suspected to be the source of the antioxidant activity was identified. The final stage was the encapsulation process. WJB kefir showed high antioxidant activity, inhibiting DPPH radicals by 90.51±4.73% and ABTS radicals by 86.63±2.34% after 72 h of fermentation. WJB kefir contained 0.35±0.01 mg GAE/g total phenolics and 0.08 mg/g total flavonoids. The LC/MS identification suggested that the bioactive antioxidant components of the WJB kefir were from the alkaloid, saponin, phenolic, and flavonoid groups. The encapsulation with maltodextrin using freeze drying resulted in microencapsulation of WJB kefir with a particle size of 6.42±0.13 μm. The encapsulation efficiency was 79.61%, and the IC 50 value was 32.62 ppm. The encapsulation method was able to maintain the antioxidant stability of the kefir and extend its shelf life. WJB kefir, a nondairy, lactose-free kefir, can be used as an antioxidant functional food.

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Section: Resultsmentioning

confidence: 99%

Potential of White Jack Bean (Canavalia ensiformis L. DC) Kefir as a Microencapsulated Antioxidant

Yusuf,

Kholifaturrohmah,

Nurcholis

et al. 2023

Prev. Nutr. Food Sci.

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“…The antioxidant benefits may be due to the substances’ capacity to impede lipid peroxidation and increase superoxide dismutase functionality. Superoxide dismutase is an enzyme that decreases tissue destruction by eliminating the superoxide radical in most cells when subjected to oxygen ( Pinmanee et al, 2023 ).…”

Section: Therapeutic Mechanismsmentioning

confidence: 99%

Harnessing the power of bee venom for therapeutic and regenerative medical applications: an updated review

Sadek,

Shib,

Taher

et al. 2024

Front. Pharmacol.

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Honeybees have been helpful insects since ancient centuries, and this benefit is not limited to being a honey producer only. After the bee stings a person, pain, and swelling occur in this place, due to the effects of bee venom (BV). This is not a poison in the total sense of the word because it has many benefits, and this is due to its composition being rich in proteins, peptides, enzymes, and other types of molecules in low concentrations that show promise in the treatment of numerous diseases and conditions. BV has also demonstrated positive effects against various cancers, antimicrobial activity, and wound healing versus the human immunodeficiency virus (HIV). Even though topical BV therapy is used to varying degrees among countries, localized swelling or itching are common side effects that may occur in some patients. This review provides an in-depth analysis of the complex chemical composition of BV, highlighting the diverse range of bioactive compounds and their therapeutic applications, which extend beyond the well-known anti-inflammatory and pain-relieving effects, showcasing the versatility of BV in modern medicine. A specific search strategy was followed across various databases; Web of sciences, Scopus, Medline, and Google Scholar including in vitro and in vivo clinical studies.to outline an overview of BV composition, methods to use, preparation requirements, and Individual consumption contraindications. Furthermore, this review addresses safety concerns and emerging approaches, such as the use of nanoparticles, to mitigate adverse effects, demonstrating a balanced and holistic perspective. Importantly, the review also incorporates historical context and traditional uses, as well as a unique focus on veterinary applications, setting it apart from previous works and providing a valuable resource for researchers and practitioners in the field.

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“…Immobilization is a well-known method to provide protection to sensitive substances and cells in harsh environments, rendering them more suitable for a range of sectors, such as tissue engineering, cosmetics, drug delivery, and environmental applications [ 3 ]. Thus, the system carrier + immobilized probiotic is a reasonable design for the goal of safe delivery of probiotics to the target organ (the large intestine) after having gone through aggressive environments of the stomach and the small intestine.…”

Section: Introductionmentioning

confidence: 99%

Prebiotic Cellulose–Pullulan Matrix as a “Vehicle” for Probiotic Biofilm Delivery to the Host Large Intestine

Savitskaya,

Zhantlessova,

Kistaubayeva

et al. 2023

Polymers

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This study describes the development of a new combined polysaccharide-matrix-based technology for the immobilization of Lactobacillus rhamnosus GG (LGG) bacteria in biofilm form. The new composition allows for delivering the bacteria to the digestive tract in a manner that improves their robustness compared with planktonic cells and released biofilm cells. Granules consisting of a polysaccharide matrix with probiotic biofilms (PMPB) with high cell density (>9 log CFU/g) were obtained by immobilization in the optimized nutrient medium. Successful probiotic loading was confirmed by fluorescence microscopy and scanning electron microscopy. The developed prebiotic polysaccharide matrix significantly enhanced LGG viability under acidic (pH 2.0) and bile salt (0.3%) stress conditions. Enzymatic extract of feces, mimicking colon fluid in terms of cellulase activity, was used to evaluate the intestinal release of probiotics. PMPB granules showed the ability to gradually release a large number of viable LGG cells in the model colon fluid. In vivo, the oral administration of PMPB granules in rats resulted in the successful release of probiotics in the colon environment. The biofilm-forming incubation method of immobilization on a complex polysaccharide matrix tested in this study has shown high efficacy and promising potential for the development of innovative biotechnologies.

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Purification and Immobilization of Superoxide Dismutase Obtained from Saccharomyces cerevisiae TBRC657 on Bacterial Cellulose and its Protective Effect against Oxidative Damage in Fibroblasts

Cited by 9 publications

References 47 publications

Potential of White Jack Bean (Canavalia ensiformis L. DC) Kefir as a Microencapsulated Antioxidant

Potential of White Jack Bean (Canavalia ensiformis L. DC) Kefir as a Microencapsulated Antioxidant

Harnessing the power of bee venom for therapeutic and regenerative medical applications: an updated review

Prebiotic Cellulose–Pullulan Matrix as a “Vehicle” for Probiotic Biofilm Delivery to the Host Large Intestine

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