1980
DOI: 10.1128/iai.30.1.140-146.1980
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Purification and immunological characterization of a rhamnose-glucose antigen from Streptococcus mutans 6517-T2 (serotype g)

Abstract: The serotype antigens of Streptococcus mutans have been described as cell wall-associated polysaccharides. In this study, an additional wall polysaccharide antigen was purified and characterized from S. mutans strain 6715-T2, a mutant of 6715 (serotype g). Strain 6715-T2 lost the serotype antigen during animal passage. Rhamnose-containing carbohydrate fractions were solubilized from bacterial cells by extraction with 5% trichloroacetic acid at 40C for 18 h and with 0.01 N HOl at 100C for 20 min. Extracts were … Show more

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Cited by 12 publications
(12 citation statements)
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“…These results indicate that a rhamnose-containing polysaccharide comprises the core of the S. mutans carbohydrate antigen, which might be involved in the linkage of serotype g carbohydrate (galactose-glucose heteropolymer) and the peptidolycan basal layer ofthe cell wall, as has been suggested for C-polysaccha-ride peptidoglycan linkages in group A S. pyogenes cell walls (29). A glucoserhamnose antigen, comparable to the carbohydrate moiety of Ml PG, was recently isolated from a mutant strain (T2) of S. mutans 6715 which had lost its serotype specificity during animal passage (30). A similar mutant of S. mutans 6715 has been isolated and characterized immunochemically to demonstrate the absence of the serotype-specific carbohydrate determinant (20).…”
Section: Properties Of the Peptidoglycan Portion Isolated From Cell Wmentioning
confidence: 93%
“…These results indicate that a rhamnose-containing polysaccharide comprises the core of the S. mutans carbohydrate antigen, which might be involved in the linkage of serotype g carbohydrate (galactose-glucose heteropolymer) and the peptidolycan basal layer ofthe cell wall, as has been suggested for C-polysaccha-ride peptidoglycan linkages in group A S. pyogenes cell walls (29). A glucoserhamnose antigen, comparable to the carbohydrate moiety of Ml PG, was recently isolated from a mutant strain (T2) of S. mutans 6715 which had lost its serotype specificity during animal passage (30). A similar mutant of S. mutans 6715 has been isolated and characterized immunochemically to demonstrate the absence of the serotype-specific carbohydrate determinant (20).…”
Section: Properties Of the Peptidoglycan Portion Isolated From Cell Wmentioning
confidence: 93%
“…The cell walls of Streptococcus sobrinus (formerly S. mutans serotypes d and g) (2) possess two distinct polysaccharide antigens, the galactose-glucose polymers, which have been used in the serological classification of these organisms (9,15), and the rhamnose-glucose polymers (RGPs), which compose 40 to 50% of the dry weight of the cell walls (13,18). The RGPs of strains B13 and 6715-T2 (representing serotypes d and g, respectively) have previously been shown to be antigenic and appear to be chemically and serologically identical (17,18). The present study used analysis by gas chromatography-mass spectroscopy to verify the structural identity of the RGPs in organisms of the d and g serotypes.…”
mentioning
confidence: 99%
“…Batch cultures of S. sobrinus B13 and 6715-T2 were grown in dialysate of Todd-Hewitt broth supplemented with glucose and salts (17). Cultures were grown at 37°C for 18 h, harvested by centrifugation, washed twice with distilled water, and lyophilized.…”
mentioning
confidence: 99%
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“…Immunochemical studies have reported that the typing antigens of the c and d strains are wall-associated polysaccharides (9,11,17) that are chemically and serologically distinct. However, immunological cross-reactions have been noted between anti-whole cell serotype c serum and a second wall polysaccharide, the rhamnose-glucose polysaccharide (RGP) that has been characterized by this laboratory in strains of S. mutans serotypes d and g (14,15). Because of the importance of serological methods in the identification of S. mutans and because of the interest in the potential protective effects of antibodies to surface antigens of S. mutans with respect to dental caries, we sought to clarify these observed cross-reactions between serotypes c and d.…”
mentioning
confidence: 99%