Purification and molecular characterization of a novel mannose‐specific lectin from Dioclea reflexa hook seeds with inflammatory activity

Abstract

A novel lectin present in Dioclea reflexa seeds (DrfL) was discovered and described in this study. DrfL was purified in a single step by affinity chromatography in a Sephadex G-50 column. The lectin strongly agglutinated rabbit erythrocytes and was inhibited by α-methyl-D-mannoside, D-mannose, and D-glucose. The hemagglutinating activity of DrfL is optimum at pH 5.0-7.0, stable up to 50 °C, and dependent on divalent cations. Similar to other lectins of the subtribe Diocleinae, the analysis by mass spectrometry indicated that DrfL has three chains (α, β, and γ) with masses of 25,562, 12,874, and 12,706 Da, respectively, with no disulfide bonds or glycosylation. DrfL showed inflammatory activity in the paw edema model and exhibited low cytotoxicity against Artemia sp.

A new lectin from Crotalaria incana seeds and studies of toxicity in Artemia salina nauplii

“…Other lectins of the same botanical family show maximum activity at similar temperatures, such as the Collaea speciosa lectin isolated by Oliveira et al (2021) and the lectin isolated by Alves et al (2015) from seeds of Clathrotropis nitida, which showed low hemagglutinating activity at 100 ºC. Total dependence of CiL on divalent ions, as noted above, closely resembled the findings of Pinto-Junior et al (2016) who isolated a metal-dependent lectin from the seeds of Dioclea reflexa. These data diverge from those of other lectins, such as Dioclea lasiophylla (lectin II -specific lactose) (Cavada et al 2020) and Canavalia virosa (Osterne et al 2014).…”

Purification and characterization of a highly thermostable GlcNAc-binding lectin from Collaea speciosa seeds

“…The lethality test using Artemia is a simple preliminary assay to determine toxicity of CsL against cells [17,62,63,64]. When toxicity of CsL for Artemia was examined using lectin concentrations ranging between 3.125 and 200 μg/mL, it was found that this lectin is innocuous to Artemia sp.…”

ConA-Like Lectins: High Similarity Proteins as Models to Study Structure/Biological Activities Relationships

“…Edematogenic activity has been studied in ConA [31], ConGF [35], ConBr [29], Canavalia gladiata lectin (CGL) [29], C. maritima lectin (ConM) [29], C. virosa lectin (ConV) [36], C. oxyphylla lectin (CoxyL) [19], C. villosa lectin (CvilL) [20], Dioclea rostrata lectin (DRL) [37], D. wilsonii lectin (DWL) [38,39], D. virgata lectin (DvirL) [40], D. lasiophylla lectin (DlyL) [41], D. reflexa lectin (DrfL) [41] and Cymbosema roseum lectin (CRL1) [42] (Table 1). Anti-inflammatory effect has been studied in ConGF [43], DvirL [33], CRL1 [42], D. guianensis lectin (DguiL), D. violacea lectin (DVL) and Cratylia floribunda lectin (CFL) [28].…”