Purification and partial characterization of the antiviral protein from Phytolacca americana which inhibits eukaryotic protein synthesis

Irvin, James D.

doi:10.1016/0003-9861(75)90195-2

Cited by 181 publications

(83 citation statements)

References 8 publications

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“…1 is a 29-kDa naturally occurring protein isolated from the leaves of the pokeweed plant, Phytolacca americana (1)(2)(3). PAP belongs to a family of plant ribosome-inactivating proteins (RIPs) that catalytically depurinate ribosomal RNA (3,4).…”

Section: Pokeweed Antiviral Protein (Pap)mentioning

confidence: 99%

Modeling and Alanine Scanning Mutagenesis Studies of Recombinant Pokeweed Antiviral Protein

Rajamohan¹,

Pugmire²,

Kurinov³

et al. 2000

Journal of Biological Chemistry

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The Phytolacca americana-derived naturally occurring ribosome inhibitory protein pokeweed antiviral protein (PAP) is an N-glycosidase that catalytically removes a specific adenine residue from the stem loop of ribosomal RNA. We have employed molecular modeling studies using a novel model of PAP-RNA complexes and site-directed mutagenesis combined with bioassays to evaluate the importance of the residues at the catalytic site and a putative RNA binding active center cleft between the catalytic site and C-terminal domain for the enzymatic deadenylation of ribosomal RNA by PAP. substantially reduced the depurinating and ribosome inhibitory activity of PAP. These results provide unprecedented evidence that besides the active site residues of PAP, the conserved, charged, and polar side chains located at its active center cleft also play a critical role in the PAPmediated depurination of ribosomal RNA.

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Section: Pokeweed Antiviral Protein (Pap)mentioning

confidence: 99%

Modeling and Alanine Scanning Mutagenesis Studies of Recombinant Pokeweed Antiviral Protein

Rajamohan¹,

Pugmire²,

Kurinov³

et al. 2000

Journal of Biological Chemistry

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“…These proteins can be classified into two groups: proteins composed of two functionally different subunits like ricin [l] and abrin [2] and those having no subunits like gelonin [3], momordin [4], and PAP [5,6]. In the former proteins, one subunit, the B-chain, binds to the cell surface and heips to transfer into the cell the other subunit, the A-chain, which, once internalized, inhibits protein synthesis.…”

Section: Introductionmentioning

confidence: 99%

Protein‐synthesis inhibitory protein from seeds of Luffa cylindria roem

1983

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A protein (designated as Iuffin) with an app. I%& of 26~, which inhibits protein synthesis in rabbit reticulocyte lysate, was purified to homogeneity from the seeds of Luffa cylindria roem by extraction with 20 mM Na phosphate buffer (pH 7.2) containing 0.2 M NaCl, ammonium sulfate fractionation, and chromatography on Sephacryl S-200 and CM-Sephadex C-25. Luffin exhibited IO-times as strong inhibitory activity against protein synthesis in rabbit reticulocyte lysate (K'SO, 0.42 ng/ml) as that of ricin A-chain, but it showed only a weak cytotoxicity against murine leukemia L1210 cells, an activity of l/lo6 to l/lo5 that of ricin.Luffa cylindria roem puffin Protein synthesis Inhibition of protein synthesis Rabbit reticulocyte lysate Cytotoxic~ty

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“…The biological effects credited to these protein toxins go back to early times, owing to the high toxicities of the castor bean and jequirity bean [10]. Yet other plants, such as American pokeweed (P. americana) and common soapwort, synthesize pokeweed antiviral protein (PAP) and saporin, that impose lower toxicity on intact cells while inhibiting protein production in cell-free lysates [11][12][13]. RIPs exert their cytotoxicity by impairing ribosomes [14]; specifically they interfere with the advanced stages of translation [15][16][17][18][19].…”

Section: Ribosome Inactivating Proteins (Rips)mentioning

confidence: 99%

“…It has been postulated that a direct interaction of PAP with viral RNA (or DNA) is an alternative antiviral mechanism in play. The pokeweed plant produces several isozymes of PAP, all exerting potent antiviral properties [11,13,[36][37][38][39][40][41][42]. PAP isozymes evoke depurination of genomic HIV-1 RNA [43][44][45], TMV RNA [46], poliovirus [47], herpes simplex virus (HSV) [48], influenza virus [49], and brome mosaic virus (BMV) [50], among many others, showing a broad spectrum of antiviral activity [13].…”

Section: Activities Attributed To Papmentioning

confidence: 99%

Insights into the Molecular Antiviral Mechanism of Pokeweed Protein from Phytolacca americana

Aitbakieva¹,

Domashevskiy²

2016

Biochem Pharmacol (Los Angel)

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Purification and partial characterization of the antiviral protein from Phytolacca americana which inhibits eukaryotic protein synthesis

Cited by 181 publications

References 8 publications

Modeling and Alanine Scanning Mutagenesis Studies of Recombinant Pokeweed Antiviral Protein

Modeling and Alanine Scanning Mutagenesis Studies of Recombinant Pokeweed Antiviral Protein

Protein‐synthesis inhibitory protein from seeds of Luffa cylindria roem

Insights into the Molecular Antiviral Mechanism of Pokeweed Protein from Phytolacca americana

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