1988
DOI: 10.1002/jnr.490190212
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Purification and partial characterization of brain adenosine deaminase: Inhibition by purine compounds and by drugs

Abstract: Rat brain adenosine deaminase (E.C. 3.5.4.4.) was purified 667-fold from the supernatant fraction by the following techniques: heat treatment (60 degrees C), fractionation with ammonium sulfate, column chromatography on DEAE-Sepharose, and preparative gel electrophoresis. The purified enzyme was homogeneous by the criterion of polyacrylamide disc gel electrophoresis and isoelectric focusing. Amino acid composition is given. The isoelectric point of the enzyme (5.2) was determined by isoelectric focusing on aga… Show more

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Cited by 51 publications
(30 citation statements)
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“…All solids were dissolved when the reaction was heated to 90 °C. The reaction mixture was then stirred under these conditions for another 12 h and volatile residues were removed under reduced pressure, followed by oil pump vacuum for 24 h. The solid residues were subject to recrystallization in hot water to give pure [6-13 C], [6][7][8][9][10][11][12][13][14][15] C]-labeled adenosines were prepared from radiolabeled riboses 7 and isotopically labeled adenines through two one-pot enzymatic reactions. Specifically labeled ATPs were synthesized as described previously with some modification.…”
Section: Synthesismentioning
confidence: 99%
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“…All solids were dissolved when the reaction was heated to 90 °C. The reaction mixture was then stirred under these conditions for another 12 h and volatile residues were removed under reduced pressure, followed by oil pump vacuum for 24 h. The solid residues were subject to recrystallization in hot water to give pure [6-13 C], [6][7][8][9][10][11][12][13][14][15] C]-labeled adenosines were prepared from radiolabeled riboses 7 and isotopically labeled adenines through two one-pot enzymatic reactions. Specifically labeled ATPs were synthesized as described previously with some modification.…”
Section: Synthesismentioning
confidence: 99%
“…Generation of Meisenheimer complex is followed by [ Table 2). The primary [6][7][8][9][10][11][12][13][14][15] (Figure 3). The hydroxyl attack and sp 3 rehybridization at C6 make the N6 electrostatic potential more negative in the transition states with overall charges of -0.912 to -0.925.…”
Section: α-Primary [6-15 N] Kiesmentioning
confidence: 99%
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“…Therefore, *To whom correspondence should be addressed. Tel: 0098-21-6498819; Fax: 0098-21-6404680 E-mail: saboury@chamran.ut.ac.ir the modulation of ADA with the use of highly specific inhibitors might modify the action of endogenous adenosine under various physiological and pathological conditions (Agarwal, 1982;Phillis et al, 1985;Centelles et al, 1988). In this study, we investigated the inhibitory effect of the inosine product on the enzymatic reaction of ADA by spectroscopy and calorimetric methods.…”
Section: Introductionmentioning
confidence: 99%