Purification and Partial Structural Characterization of a Complement Fixating Polysaccharide from Rhizomes of Ligusticum chuanxiong

Abstract

Rhizome of Ligusticum chuanxiong is an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China. Polysaccharides from this plant have been shown to have interesting bioactivities, but previous studies have only been performed on the neutral polysaccharides. In this study, LCP-I-I, a pectic polysaccharide fraction, was obtained from the 100 °C water extracts of L. chuangxiong rhizomes and purified by diethylaminethyl (DEAE) sepharose anion exchange chromatography and gel filtration. Monosaccharide analysis and linkage determination in addition to Fourier transform infrared (FT-IR) spectrometer and Nuclear magnetic resonance (NMR) spectrum, indicated that LCP-I-I is a typical pectic polysaccharide, with homo-galacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and type II (AG-I/AG-II) side chains. LCP-I-I exhibited potent complement fixation activity, ICH50 of 26.3 ± 2.2 µg/mL, and thus has potential as a natural immunomodulator.

Structure elucidation of a pectin fromDendrobium nobileLindl. and its immunological activity

“…Moreover, the substituted groups play an important role in the expression of biological activities [9,10]. In the food industry, pectic polysaccharides are used as thickener, stabilizer, gelling agent, emulsifier, flavour fixation agent and texture modifier [1,11,12]. Pectic polysaccharides are widely studied due to their bioactivities and applications, such as pectin from Panax ginseng [13], Centella asiatica [4] and Diospyros kaki Thumb [14], etc.…”

Purification, Characterization, and Antioxidant Activity of Polysaccharides Isolated from Cortex Periplocae

“…The signal peak at 98.57 ppm was confirmed to be the anomeric carbon of α-d-Glc p [ 34 , 36 ]. In the 13 C-NMR, the chemical shifts between 107.3–109.3 ppm were indicated to be Ara f ; the chemical shifts between 102.9–104.2 ppm were indicated to be Gal p and Glc p [ 35 , 37 ]. The 13 C signal from 67 to 70 ppm indicated the existence of (1 → 6) glycosidic linkages; the signals from 80 to 83 ppm confirmed the existence of (1 → 3/4) glycosidic linkages [ 35 ].…”

Fabrication of Ligusticum chuanxiong polylactic acid microspheres: A promising way to enhance the hepatoprotective effect on bioactive ingredients

“…(stretching vibration of C=O), 1665 cm -1 (stretching vibrations of -C=N) and 992 cm -1 (C=C) in LCE, disappeared in the LCE-PLA microspheres attributing to the adequate encapsulation process (Zou, Fu, Chen, Austarheim, Inngjerdingen, Huang, et al, 2017). The characteristic peaks at 1391 cm -1 (stretching vibration of -CH 3 ), 1756 cm -1…”