Purification and preparation of compounds from an extract of Scutellaria barbata D. Don using preparative parallel high performance liquid chromatography

Abstract

Preparative parallel high performance liquid chromatography combined with solvent partition and other pretreatments were adopted to separate and purify compounds from an extract of Scutellaria barbata D. Don. Mass-triggered fraction collection allowed the rapid and precise isolation of target compounds. Twelve compounds were isolated from the extract of S. barbata D. Don, their purity in area percent was determined by HPLC analysis, and the structures of seven compounds were further identified with HPLC/ESI-MS, (1)H NMR, and( 13)C NMR, among which 4-(3,4-dihydroxy-phenyl)-but-3-en-2-one, acacetin-7-diglucuronide, and luteolin-7-diglucuronide were the first to be identified from this plant. The results demonstrated that multi-channel parallel preparative HPLC/UV/MS is an efficient method for isolation and purification of compounds from natural products.

Correlation between plasma antioxidant capacity and verbascoside levels in rats after oral administration of lemon verbena extract

“…Moreover, one methoxylated flavone, chrysoeriol-7-diglucuronide is reported for the first time in lemon verbena. Total mass data and fragmentation patterns of these flavones were identical to those ones reported in other plant sources (Wang et al, 2008).…”

BZL101, a phytochemical extract from theScutellaria barbataplant, disrupts proliferation of human breast and prostate cancer cells through distinct mechanisms dependent on the cancer cell phenotype

“…The chemical composition of BZL101 is complex [29][30][31] and has been shown to contain several anticancer phytochemicals such as apigenin, luteolin and resveratrol, 29,32 that can disrupt multiple proliferative pathways. In addition, this herbal mixture was recently shown to contain several anticancer flavones, such as Wogonin, Baicalein and Baicalin, which have both cytostatic and cytotoxic activities in certain cultured cells and can inhibit tumor growth in vivo.…”

Bioassay-Guided Separation and Identification of a New Anti-Lung Cancer Compound from Cordyceps militaris by Means of Off-Line Two-Dimensional Preparative Chromatography, Real-Time Cell Analysis, and X-ray Single-Crystal Diffraction

“…It was also worthy of note that heart-cutting was used as the repeated separation strategy to ensure the purity of the compounds. Good separation resulted in seven major peaks labeled from Fraction 10-1 to Fraction 10-7, eluting at 6,8,11,14,17,26 and 30 min, respectively (Fig. 1e).…”

“…Fourteen fractions were collected and dried by rotary evaporation at 50 °C. Figure 1b shows four major and eight minor peaks eluting at 6,7,8,10,15,17,18,19,20,22,27,34,36, and 40 min; the amounts by weight ranged from approximately 0.08 g for fraction 9 to 4.89 g for fraction 10. Recovery from the bare silica DAC preparative column was approximately 68 %.…”

“…However, because of successive solvent extractions and successive column isolation, low column efficiency, poor separation repeatability and poor column resolution, fractions with low pharmacological activity, only, have been obtained. Preparative high-performance liquid chromatography (PHPLC), one of the most effective methods for isolation and purification of active compounds from natural products, takes advantage of high-performance separation, online monitoring, and automatic control to achieve efficient isolation of single compounds [13][14][15][16][17]. However, because of the complex chemical composition of TCM extracts, it is impossible to obtain compounds of high purity by one-dimensional separation only, because of relatively low column efficiency and peak capacity, and limited selectivity.…”