Purification and properties of a haemolysin produced by group A haemolytic streptococci (streptolysin O)

Herbert, D.; Todd, E. W.

doi:10.1042/bj0351124

Cited by 72 publications

(48 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar level was also published for recombinant SLO (32 -571 amino acid sequence) (22). The mature SLO (contain 32 -571 amino acid) fused with His-tag had a specific activity (748,000 HU/mg protein) and its activity was identical to mature natural or recombinant SLO (16).…”

Section: The Pectate Lyase Could Strengthen the Hemolysis Activity Ofsupporting

confidence: 62%

Pectate Lyase Promoting Streptolysin O Expression in Escherichia coli and Strengthening Its Activity

Qingxin

Kaixia

et al. 2017

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Streptolysin O (SLO) from Streptococcus pyogenes could oligomerize to form large pores on the membrane of some eukaryotic cells. Because of the action, SLO had multifaceted applications in animal cell biology and medicine science. In the previous researches, the highest yield of recombinant expressed SLO might be no more than 124 mg/L medium. Objectives: A fusion protein of pectate lyase and streptolysin O (PEL-SLO) was highly expressed with the pectate lyase (PEL, from

show abstract

Section: The Pectate Lyase Could Strengthen the Hemolysis Activity Ofsupporting

confidence: 62%

Pectate Lyase Promoting Streptolysin O Expression in Escherichia coli and Strengthening Its Activity

Qingxin

Kaixia

et al. 2017

Jundishapur J Microbiol

View full text Add to dashboard Cite

show abstract

“…SLO, an oxygen-sensitive hemolysin, was first described by Neil and Mallory in 1928 and subsequently confirmed by Herbert and Todd in 1941. 27 Multiple roles in virulence have been attributed to SLO. Van Epps and Andersen 28 showed that SLO inhibits neutrophil chemotaxis and mobility.…”

Section: Discussionmentioning

confidence: 99%

Contribution of Secreted NADase and Streptolysin O to the Pathogenesis of Epidemic Serotype M1 Streptococcus pyogenes Infections

Zhu

Olsen

Lee

et al. 2017

The American Journal of Pathology

View full text Add to dashboard Cite

Streptococcus pyogenes secretes many toxins that facilitate human colonization, invasion, and dissemination. NADase (SPN) and streptolysin O (SLO) are two toxins that play important roles in pathogenesis. We previously showed that increased production of SPN and SLO in epidemic serotype M1 and M89 S. pyogenes strains is associated with rapid intercontinental spread and enhanced virulence. The biological functions of SPN and SLO have been extensively studied using eukaryotic cell lines, but the relative contribution of each of these two toxins to pathogenesis of epidemic M1 or M89 strains remains unexplored. Herein, using a genetically representative epidemic M1 strain and a panel of isogenic mutant derivative strains, we evaluated the relative contributions of SPN and SLO toxins to virulence in mouse models of necrotizing myositis, bacteremia, and skin and soft tissue infection. We found that isogenic mutants lacking SPN, SLO, and both toxins are equally impaired in ability to cause necrotizing myositis. In addition, mutants lacking either SPN or SLO are significantly attenuated in the bacteremia and soft tissue infection models, and the mutant strain lacking production of both toxins is further attenuated. The mutant strain lacking both SPN and SLO production is severely attenuated in ability to resist killing by human polymorphonuclear leukocytes. We conclude that both SPN and SLO contribute significantly to S. pyogenes pathogenesis in these virulence assays. (Am J Pathol 2017, 187: 605e613; http://dx.doi.org/10.1016/j.ajpath.2016.11.003) Streptococcus pyogenes (alias group A Streptococcus) is a major bacterial pathogen causing human morbidity and mortality globally. Streptococcus pyogenes produces many virulence factors that facilitate transmission, colonization, invasion, and dissemination.1 NADase (SPN) and streptolysin O (SLO) are two potent cytotoxins secreted by S. pyogenes, and multiple roles in virulence have been attributed to each protein. SPN is actively translocated into the host cells, resulting in cell injury and death via depletion of host cell NAD þ . 2e4 SLO is a cholesteroldependent cytolysin that forms pores in host cell membranes. SPN and SLO inhibit phagocytosis, 3 inhibit maturation of autophagosomes, 5,6 impair neutrophil oxidative burst, 7 and prevent the killing of S. pyogenes by a variety of host immune cells. 3,5e8 SPN and SLO are interconnected in many ways. Several lines of evidence suggest that the SPN and SLO proteins interact physically.4,9e11 Moreover, SPN and SLO toxins are functionally related. For example, translocation of SPN into host cells requires SLO.2 In addition, binding of SPN to the host cell membrane promotes SLOmediated pore formation.

show abstract

“…The properties of these hemolysins are described in the papers of Todd (10) and Herbert and Todd (11,12).…”

Section: Probable Identity Of Nucleic Acid-induced Streptolysin With mentioning

confidence: 99%

The Effect of Nucleic Acids and of Carbohydrates on the Formation of Streptolysin

Bernheimer

Rodbart

1948

Journal of Experimental Medicine

View full text Add to dashboard Cite

Yeast nucleic acid has been shown by Okamoto (1) to induce the formation of a potent hemolysin in cultures of Streptococcus pyogenes. As reported b y Okamoto, and confirmed in the present paper, the effect of nucleic acid is readily demonstrable since the hemolytic activity of filtrates or supernates of nucleic acid-broth cultures is at least 20 to 100 times greater than the activity of cultures prepared in broth to which no nucleic acid has been added. On blood agar containing yeast nucleic acid, the effect is manifest by the appearance of very large zones of beta hemolysis around the colonies.The nucleic acid effect has been investigated further in order to define the conditions necessary for producing the hemolysin on a large scale and in a manner which will facilitate its purification. In addition, although some of the properties of the nucleic acid-induced hemolysin have been described b y It6 (2), the relationship of this hemolysin to the better characterized streptococcal hemolysins, streptolysin O and streptolysin S, requires clarification. Finally, an opportunity is afforded of studying an almost unknown biological effect of nucleic acid. Methods, Materials, and TerminologyPreparation of Peptone-Infusion Broth.--Fresh beef hearts, freed of gross fat, were ground and then mixed well with tap water, 1 liter of water for each pound of ground heart. Mter being brought to 85°C. and infused at that temperature for 45 minutes, the mixture was filtered while hot, through paper. To each liter of infusion was added 10 gin. peptone (neopeptone Difco) and 5 gin. reagent sodium chloride. After the ingredients were completely dissolved by bringing to a boil, the pI-I was brought to 7.9, the medium was boiled 1 to 2 minutes, and then filtered immediately through paper. The medium was sterilized in the autoclave at 121°C. for 15 to 30 minutes. Immediately before inoculating, a freshly prepared solution of sodium thioglycollate was added to give a final concentration of 0.01 per cent.Culture Technique.--A 6 to 8 hour peptone-infusion broth culture of Streptococcus pyogenes, strain C203S, was distributed in 0.5 ce. amounts among a large number of sterile tubes, and then rapidly frozen solid in an alcohol-carbon dioxide freezing mixture. The tubes were stored in a dry-ice chest until needed. When required, a tube was thawed at room temperature, the contents diluted 10 times in sterile saline, and 0.1 cc. inoculated into each 10 cc. of

show abstract

Purification and properties of a haemolysin produced by group A haemolytic streptococci (streptolysin O)

Cited by 72 publications

References 6 publications

Pectate Lyase Promoting Streptolysin O Expression in Escherichia coli and Strengthening Its Activity

Pectate Lyase Promoting Streptolysin O Expression in Escherichia coli and Strengthening Its Activity

Contribution of Secreted NADase and Streptolysin O to the Pathogenesis of Epidemic Serotype M1 Streptococcus pyogenes Infections

The Effect of Nucleic Acids and of Carbohydrates on the Formation of Streptolysin

Contact Info

Product

Resources

About