Purification and properties of a novel extra-cellular thermotolerant metallolipase of Bacillus coagulans MTCC-6375 isolate

Kanwar, Shamsher S.; Ghazi, Iraj; Chimni, Swapandeep Singh; Joshi, Gopal; Rao, G. V.; Kaushal, Rajeev; Gupta, Reena; Punj, Vasu

doi:10.1016/j.pep.2005.10.007

Cited by 40 publications

(23 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The maximum lipase activity 18.56 U/g was observed at 45 and the lipase activity increased with an increase in temperature but the increase in temperature beyond 45 affected the lipase activity. The obtained result was similar to that for the temperature reported for B. coagulans MTCC-6375 Kanwar et al, 2006 . The temperature regulates the extracellular enzyme production by changing the physical properties of the cell membrane Lima et al, 2003 .…”

Section: Identification Of the Isolated Strainsupporting

confidence: 89%

Utilization of Coconut Oil Cake for the Production of Lipase Using <i>Bacillus coagulans</i> VKL1

Palanisamy¹,

Muthukumar²,

Velan³

2015

Biocontrol Sci.

View full text Add to dashboard Cite

The overproduction of enzymes was performed by manipulating the medium components. In our study, solvent-tolerant thermophilic lipase-producing Bacillus coagulans was isolated from soil samples and a stepwise optimization strategy was employed to increase the lipase production using coconut oil cake basal medium. In the first step, the influence of pH, temperature, carbon source, nitrogen source and inducers on lipase activity was investigated by the One-Factor-At-A-Time OFAT method. In the second step, the three significant factors resulted from OFAT were optimized by the statistical approach CCD . The optimum values of olive oil 0.5 , Tween 80 0.6 and FeSO 4 0.05 was found to be responsible for a 3.2-fold increase in the lipase production identified by Central Composite Design.

show abstract

Section: Identification Of the Isolated Strainsupporting

confidence: 89%

Utilization of Coconut Oil Cake for the Production of Lipase Using <i>Bacillus coagulans</i> VKL1

Palanisamy¹,

Muthukumar²,

Velan³

2015

Biocontrol Sci.

View full text Add to dashboard Cite

show abstract

“…One of the possibilities is that BSA used contains impurities that act as lipase activators. The enhancement of lipase activity by calcium ions has been widely described [27][28][29][30]. The effect of calcium ions is generally assigned to precipitation of calcium salts of fatty acids released in the enzymatic hydrolysis, therefore avoiding product inhibition.…”

Section: Effect Of Bovine Serum Albuminmentioning

confidence: 99%

Enzyme Substrates Protective Encapsulation within Polymeric Microspheres

Zajda¹,

Jastrzębska²,

Olszyna³

et al. 2013

AJAC

View full text Add to dashboard Cite

Determination of enzymes activity is an important task of analytical and biomedical oriented fluorimetry. Despite of a long track record of application, there is still some room for improvements in this field. In the case of hydrolases, spontaneous decomposition of substrate leads to substantial errors in determination of enzyme activity. An innovative and effective approach is proposed allowing protection of enzyme substrate within the lipophilic moiety of polyacrylate microspheres. It is shown that the introduction of substrate into microspheres is not only an effective method of prevention of unwanted spontaneous process, but also does not disturb the availability of substrate for enzymatic hydrolysis. The effect of presence of proteins in the sample on fluorimetric responses was studied. In contrary to previous studies related to application of lipophilic polymers in biomedical analysis, it is shown that the presence of bovine serum albumins enhances the sensitivity of fluorimetric determination. It is shown that this surprising effect is related to adsorption of proteins on the microspheres surface and change of surface charge of polymer.

show abstract

“…The concentration of saturation (60-70%) has already been described in the literature as adequate for precipitation of enzymes produced by different microorganisms and substrates [8,[15][16][17][18]. Lipases produced by Pseudomonas aeruginosa [19], Pseudomonas fluorescens [20], Aspergillus niger [21], Antrodia cinnamomea [8], and Bacillus sp.…”

Section: Concentration Of Crude Enzymatic Extractsmentioning

confidence: 99%

“…RSJ-1 [17] were concentrated using ammonium sulfate at 80% of saturation. Extracts obtained from Bacillus coagulans MTCC-6375 [16] and A. cinnamomea [8] could be concentrated at 30% of saturation, whereas 40% of saturation was used for lipases from Clostridium tetanomorphum [22] and 20% for that obtained from Favobacterium odoratum [23]. The concentration of crude lipase extract of Pseudomonas sp.…”

Section: Concentration Of Crude Enzymatic Extractsmentioning

confidence: 99%

Concentration, Partial Characterization, and Immobilization of Lipase Extract from P. brevicompactum by Solid-State Fermentation of Babassu Cake and Castor Bean Cake

Silva

Freire²,

Castro

et al. 2011

Appl Biochem Biotechnol

View full text Add to dashboard Cite

One relevant limitation hindering the industrial application of microbial lipases has been attributed to their production cost, which is determined by the production yield, enzyme stability among other. The objective of this work was to evaluate the concentration and immobilization of lipase extracts from Penicillium brevicompactum obtained by solid-state fermentation of babassu cake and castor bean cake. The precipitation with ammonium sulfate 60% of saturation of crude extract obtained with babassu cake as raw material showed an enhancement in hydrolytic and esterification activities from 31.82 to 227.57 U/g and from 170.92 to 207.40 U/g, respectively. Concentrated lipase extracts showed preference to medium-chain triglycerides and fatty acids. It is shown that the enzyme activity is maintained during storage at low temperatures (4 and -10°C) for up to 30 days. Higher esterification activities were achieved when the lipase extract was immobilized in sodium alginate and activated coal.

show abstract

Purification and properties of a novel extra-cellular thermotolerant metallolipase of Bacillus coagulans MTCC-6375 isolate

Cited by 40 publications

References 26 publications

Utilization of Coconut Oil Cake for the Production of Lipase Using <i>Bacillus coagulans</i> VKL1

Utilization of Coconut Oil Cake for the Production of Lipase Using <i>Bacillus coagulans</i> VKL1

Enzyme Substrates Protective Encapsulation within Polymeric Microspheres

Concentration, Partial Characterization, and Immobilization of Lipase Extract from P. brevicompactum by Solid-State Fermentation of Babassu Cake and Castor Bean Cake

Contact Info

Product

Resources

About