Purification and properties of a heat-stable exoinulinase isoform from Aspergillus fumigatus

Gill, Prabhjot Kaur; Manhas, Rajesh Kumari; Singh, Prabhjeet

doi:10.1016/j.biortech.2005.04.034

Cited by 60 publications

(38 citation statements)

References 35 publications

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“…Cataña et al [26] determined a value for K m of 82 mM for sucrose hydrolysis using the free enzyme and of 184 mM for the immobilised enzyme. Gill et al [1] determined a value for K m of 33.33 mM for sucrose hydrolysis using a purified inulinase from Aspergillus fumigatus.…”

Section: Assay Of the Kinetics Parametersmentioning

confidence: 99%

“…The purification yield achieved was 74% with a purification factor of 10.4. Gill et al [1], studying the purification process of the inulinase obtained from Aspergillus fumigatus, obtained a lower yield of 30% using the ammonium sulphate fraction.…”

Section: Verification Of the Viability Of Expanded Bed Recoverymentioning

confidence: 99%

“…Fructose is considered as a safe alternative sweetener to sucrose because it shows beneficial effects in diabetic patients, increases iron absorption in children and has a higher sweetening capacity. Fructooligosaccharides have functional and nutritional properties for use in low-calorie diets, stimulation of Bifidus and as a source of dietary fibre in food preparations [1]. Both fructose and fructooligosaccharides can be produced by inulinase via the enzymatic hydrolysis of inulin, the latter being a fructose polymer found in plants such as Jerusalem artichoke, chicory and dahlia [2,3].…”

Section: Introductionmentioning

confidence: 99%

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Technical viability of the production, partial purification and characterisation of inulinase using pretreated agroindustrial residues

Treichel

Mazutti

Filho

et al. 2008

Bioprocess Biosyst Eng

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The present work aimed to study the viability of the use of sugarcane molasses and corn steep liquor (CSL) in a sequential inulinase production performing an up-stream pretreatment of these agroindustrial residues. A sequential strategy was used applying three central composite rotatable designs (CCRDs) to optimise medium composition, followed by a down-stream step. The medium containing 150 g L(-1) molasses, 50 g L(-1) CSL and 6 g L(-1) yeast extract, yielded a maximum inulinase production of 1,294 +/- 7 U mL(-1), after 72 h of fermentation. A down-stream evaluation was carried out using an expanded bed of Streamline DAE resin (Pharmacia), with and without the up-stream treatment. The results showed that the enzyme could not be recovered from the non-pretreated medium, whereas a yield of 91% was obtained in the adsorption stage from the medium prepared with the up-stream treatment, showing the viability of producing the enzyme inulinase from agroindustrial residues using the integrated process.

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Section: Assay Of the Kinetics Parametersmentioning

confidence: 99%

Section: Verification Of the Viability Of Expanded Bed Recoverymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Technical viability of the production, partial purification and characterisation of inulinase using pretreated agroindustrial residues

Treichel

Mazutti

Filho

et al. 2008

Bioprocess Biosyst Eng

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“…The enzymatic Anais da Academia Brasileira de Ciências (2012) formation of fructose from inulin has a single enzymatic step and yields up to 95% of fructose (Figueiredo-Ribeiro et al 2007). The use of microbial inulinases (EC 3.2.1.7), which yield up to 95% of pure fructose in a single-step enzymatic reaction, is therefore a viable alternative for the production of high-fructose syrup (Gill et al 2006). Inulinases have also been used for the production of inulooligosaccharides-low caloric saccharides by acting as a growth factor for beneficial microorganisms in the intestinal flora.…”

Section: Introductionmentioning

confidence: 99%

Production, characterization and application of inulinase from fungal endophyte CCMB 328

Nascimento

Valasques

Fernandes³

et al. 2012

An. Acad. Bras. Ciênc.

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Inulinase (β-2,1-D-fructan fructanohydrolase), EC 3.2.1.7, targets the β-2,1 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose. This use provides an alternative to produce fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and applications of inulinases from the fungal endophyte CCMB 328 isolated from the Brazilian semi-arid region. Response Surface Methodology (RSM) was employed to evaluate the effect of variables (concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in the inulin hydrolysis. The results showed that the best conditions for inulinase production by CCMB 328 are 9.89 g / L for glucose and 1.09 g / L for yeast extract. The concentration of 0.20 mol/L of NaCl and KCl increased the activity of inulinase from CCMB 328 by approximately 63% and 37%, respectively. The results also showed that the inulinase has potential for inulin hydrolysis, whose conversion yields roughly 72.48 % for an initial concentration of inulin at 1% (w/v).

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“…The use of salts, such as ammonium sulfate, is frequently cited in the literature in enzyme recovery, resulting in an increase of enzymatic activities (Silva et al 2011, Gill et al 2006.…”

Section: Purification Of Inulinases 61mentioning

confidence: 99%

Purification of inulinases by changing the ionic strength of the medium and precipitation with alcohols

Golunski

Silva

Marques

et al. 2017

An. Acad. Bras. Ciênc.

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The present study evaluated the purification of inulinase by changing the ionic strength of the medium by addition of NaCl and CaCl 2 followed by precipitation with n-propyl alcohol or iso-propyl alcohol. The effects of the concentration of alcohols and the rate of addition of alcohols in the crude extract on the purification yield and purification factor were evaluated. Precipitation caused an activation of enzyme and allowed purification factors up to 2.4-fold for both alcohols. The purification factor was affected positively by the modification of the ionic strength of the medium to 0.5 mol.L -1 NaCl before precipitation with the alcohol (n-propyl or iso-propyl). A purification factor of 4.8-fold and an enzyme yield of 78.1 % could be achieved by the addition of 0.5 mol.L -1 of NaCl to the crude extract, followed by the precipitation with 50 % (v/v) of n-propyl alcohol, added at a flow rate of 19.9 mL/min.

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Purification and properties of a heat-stable exoinulinase isoform from Aspergillus fumigatus

Cited by 60 publications

References 35 publications

Technical viability of the production, partial purification and characterisation of inulinase using pretreated agroindustrial residues

Technical viability of the production, partial purification and characterisation of inulinase using pretreated agroindustrial residues

Production, characterization and application of inulinase from fungal endophyte CCMB 328

Purification of inulinases by changing the ionic strength of the medium and precipitation with alcohols

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