Purification and Properties of a β-1,3-glucanase from Chaetomium sp. that is Involved in Mycoparasitism

Sun, Hui; Yang, Jinkui; Lin, Chao; Huang, Xiaowei; Xing, Ruihuan; Zhang, Ke‐Qin

doi:10.1007/s10529-005-5132-0

Cited by 25 publications

(23 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Trichoderma koningii inhibits the growth of Sclerotium cepivorum in onion root via the production of exo- and endo-chitinases, which digest the cell walls of pathogens [42]. β-1, 3-glucanase, also associated with mycoparasitism, induces strong hydrolysis of pathogen cell walls, thereby inhibiting germination of pathogen spores and digestion of the mycelium [43]. In addition, proteases play an important role in mycoparasitism, as they are necessary for the complete hydrolysis of pathogen cell walls [44].…”

Section: Discussionmentioning

confidence: 99%

Identification of a novel fungus, Trichoderma asperellum GDFS1009, and comprehensive evaluation of its biocontrol efficacy

Sun

et al. 2017

PLoS ONE

126

View full text Add to dashboard Cite

Due to its efficient broad-spectrum antimicrobial activity, Trichoderma has been established as an internationally recognized biocontrol fungus. In this study, we found and identified a novel strain of Trichoderma asperellum, named GDFS1009. The mycelium of T. asperellum GDFS1009 exhibits a high growth rate, high sporulation capacity, and strong inhibitory effects against pathogens that cause cucumber fusarium wilt and corn stalk rot. T. asperellum GDFS1009 secretes chitinase, glucanase, and protease, which can degrade the cell walls of fungi and contribute to mycoparasitism. The secreted xylanases are good candidates for inducing plant resistance and enhancing plant immunity against pathogens. RNA sequencing (RNA-seq) and gas chromatography-mass spectrometry (GC-MS) showed that T. asperellum GDFS1009 produces primary metabolites that are precursors of antimicrobial compounds; it also produces a variety of antimicrobial secondary metabolites, including polyketides and alkanes. In addition, this study speculated the presence of six antimicrobial peptides via ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF-MS/MS). Future studies should focus on these antimicrobial metabolites for facilitating widespread application in the field of agricultural bio-control.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of a novel fungus, Trichoderma asperellum GDFS1009, and comprehensive evaluation of its biocontrol efficacy

Sun

et al. 2017

PLoS ONE

126

View full text Add to dashboard Cite

show abstract

“…NoS3 from Nerium oleander L. with strong antioxidant activity. Sun et al (2006) reported the antifungal activity of Chaetomium sp. and isolated from it an extracellular b-1, 3-glucanase.…”

Section: Identification Of 48 Strains Belonging To Sordariomycetesmentioning

confidence: 99%

Molecular identification of endophytic fungi from medicinal plant Huperzia serrata based on rDNA ITS analysis

Chen

Wei

et al. 2010

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Fifty-two endophytic fungi strains with different colony morphologies were isolated from stems, leaves and roots of Huperzia serrata (Thunb. ex Murray) Trevis. collected from Bawangling Reserve of Hainan Province in southern China. They were identified mainly based on rDNA ITS sequences and phylogenetic analysis. The results showed that all strains belonged to four classes, i.e. Sordariomycetes (92.31%), Dothideomycetes (3.85%), Pezizomycetes (1.92%) and Agaricomycetes (1.92%). Forty-seven strains were identified at the genus level, including Glomerella (Colletotrichum), Hypocrea (Trichoderma), Pleurostoma, Chaetomium, Coniochaeta (Lecythophora), Daldinia, Xylaria, Hypoxylon, Nodulisporium, Cazia and Phellinus. As to the other five strains, three were identified at the order level and two at the family level, indicating that a great diversity of fungi taxa exists in H. serrata. Most isolated strains belonged to the genus of Glomerella (Colletotrichum) and Hypoxylon, twenty-one from Glomerella and its anamorph Colletotrichum (42.3% of total isolated strains) and ten from Hypoxylon (19.2% of total isolated strains). Pleurostoma, Chaetomium, Coniochaeta (Lecythophora), Daldinia, Xylaria, Hypoxylon, Nodulisporium, Cazia and Phellinus were reported as endophytic fungi isolated from H. serrata for the first time.

show abstract

“…and R. solani, P. byssoides and plant pathogenic fungi were inoculated on the surface of a water agar (2% w/v) plate (9 cm), and they were incubated on opposite each other (6 cm) (Sun et al 2006). The interactions between P. byssoides and the plant pathogenic fungi were examined under a light microscope after culturing for 3-15 days at 27°C.…”

Section: Dual-culture Plate Assaymentioning

confidence: 99%

Purification and characterization of a β-1,3-glucanase from the novel mycoparasite Periconia byssoides

et al. 2007

Self Cite

View full text Add to dashboard Cite

An extracellular beta-1,3-glucanase with antifungal properties was secreted by the novel mycoparasite, Periconia byssoides. The glucanase has a molecular mass of 35 kDa estimated by SDS-PAGE. Its optimum activity was at pH 6.0 and 50 degrees C (over 2 h). The purified beta-1,3-glucanase was capable of degrading cell walls, and inhibiting mycelia growth and spore germination of plant pathogenic fungi including Fulvia fulva, Fusarium sp. and Rhizoctonia solani. The N-terminal amino acid residues of the purified beta-1,3-glucanase are LKNGGPSFGA, which do not have any homology with previously described glucanases, suggesting it may be a novel member of the fungal beta-1,3-glucanases.

show abstract

Purification and Properties of a β-1,3-glucanase from Chaetomium sp. that is Involved in Mycoparasitism

Cited by 25 publications

References 16 publications

Identification of a novel fungus, Trichoderma asperellum GDFS1009, and comprehensive evaluation of its biocontrol efficacy

Identification of a novel fungus, Trichoderma asperellum GDFS1009, and comprehensive evaluation of its biocontrol efficacy

Molecular identification of endophytic fungi from medicinal plant Huperzia serrata based on rDNA ITS analysis

Purification and characterization of a β-1,3-glucanase from the novel mycoparasite Periconia byssoides

Contact Info

Product

Resources

About