Purification and properties of a novel fucose-specific hemagglutinin of Aleuria aurantia

Abstract

A fucose-binding lectin from fruiting bodies of Aleuria aurantia was purified by affinity chromatography by using the H-active glycopeptide of desialyzed porcine submaxillary mucine coupled to Sepharose 4B and eluting with L-fucose. Homogeneity of the active protein was confirmed by polyacrylamide gel electrophoresis, isoelectric focusing, column chromatography using Sephadex G-100, and ultracentrifugal analyses. It has a molecular weight of 72 000 and is proposed to be a dimer of identical subunits, each of which has combining site of uniform affinity. Chemical analyses revealed the absence of the sulfur-containing amino acid and carbohydrate, neutral and amino sugar, in the lectin molecule. It agglutinated human erythrocytes of all ABO and Lewis types, Bombay phenotype, and group O cells treated with alpha (1 leads to 2)-fucosidase. In double-diffusion experiments, the lectin formed a single precipitin line which fused with all of the fucose-containing blood-group substances tested, including the alpha-fucosidase-treated materials. These findings together with the results of hemagglutination and precipitation studies indicate that the lectin combines the terminal fucose in the carbohydrate chain but doses not require a particular linkage to the penultimate sugar moiety.

Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies

“…Homodimer 4 Gal na Entomotoxic (Hamshou et al 2013;Hamshou et al 2010b;Hamshou et al 2012;Skamnaki et al 2013) Bleuler-Martinez et al 2011;Fujihashi et al 2003;Fukumori et al 1990;Kochibe and Furukawa 1980;Olausson et al 2010;Olausson et al 2008;Tateno et al 2009;Wimmerova et al 2003) GlcNAc-β1,4-X na nd (Cioci et al 2006;Ueda et al 1999a;Ueda et al 2002;Ueda et al 1999b;Ueda et al 2003) Entomotoxic (Birck et al 2004;Bleuler-Martinez et al 2011;Damian et al 2005;Francis et al 2003;Jaber et al 2008;Jaber et al 2007;…”

Carbohydrate Binding Specificity of a Fucose-specific Lectin from Aspergillus oryzae

“…To date, some lectins have been identified as fucose-specific including Lotus tetragonolobus (3) and Ulex europaeus (9) lectins from plants, Anguilla lectin from eel (10), Aleuria aurantia lectin (AAL) 2 from mushroom (11), Rhizopus stolonifer lectin from fungi (12), and Ralstonia solanacearum lectin from bacteria (13). Among these lectins, AAL and R. stolonifer lectin preferentially bind to ␣1,6-fucosylated oligosaccharides, whereas Ulex europaeus and Lotus tetragonolobus lectins prefer ␣1,2-linked fucose residues (14).…”

Immunological Properties of Arabinogalactan Proteins from Leaves of Cruciferous Plants

“…The immunoprecipitations of the cruciferous AGPs with eel anti-H precipitin, a L-fucose-specific lectin of Aleuria aurantia, 3) which was kindly provided by Dr. N. Kochibe, Gunma University, Gunma, and rabbit anti-radish leaf AGP sera were done by a double diffusion technique 4 ) in agar (1 % Difco agar purified), and agarose plate (1.5% agarose for electrophoresis, Nakarai Chern. Co., Kyoto) with 0.025 M barbital buffer, pH 8.4, or 0.0145 M phosphate buffer, pH 7.2, containing 0.13 M NaCl (PBS) in the presence of 0.01 % Trypan blue.…”