Purification and properties of a fibrinolytic enzyme fromBacillus subtilis

Fayek, K. I.; El‐Sayed, Sanaa T.

doi:10.1002/jobm.3630200603

Cited by 19 publications

(5 citation statements)

References 9 publications

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“…Acetylcholinesterase (AChE) inhibitory activity was measured spectrophotometrically using the technique of Ellman et al [24]. Fibrinolytic activity was also assayed by the method of Fayek and El-Sayed [25]. …”

Section: Methodsmentioning

confidence: 99%

Extraction and Characteristics of Anti-obesity Lipase Inhibitor fromPhellinus linteus

et al. 2010

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To develop a potent anti-obesity lipase inhibitor from mushroom, the lipase inhibitory activities of various mushroom extracts were determined. Methanol extracts from Phellinus linteus fruiting body exhibited the highest lipase inhibitory activity (72.8%). The inhibitor was maximally extracted by treatment of a P. linteus fruiting body with 80% methanol at 40℃ for 24 hr. After partial purification by systematic solvent extraction, the inhibitor was stable in the range of 40~80℃ and pH 2.0~9.0. In addition to lipase inhibitory activity, the inhibitor showed 59.4% of superoxide dismutase-like activity and 56.3% of acetylcholinesterase inhibitory activity.

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Section: Methodsmentioning

confidence: 99%

Extraction and Characteristics of Anti-obesity Lipase Inhibitor fromPhellinus linteus

et al. 2010

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“…Fibrinolytic activity was assayed by the method of Fayek and El-Sayed [10]. Each sample of 0.5 mL was added to 3 mL of a substrate solution (0.6% fibrin in 0.1M Mcllvaine buffer, pH 7.0) and incubated at 40℃ for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Growth Characteristics and Physiological Functionality of Yeasts in Pear Marc Extracts

Jang

Kang

et al. 2011

Mycobiology

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Kluyveromyces fragilis KCTC 7260 and Saccharomyces cerevisiae KCTC 7904, which both grew well in pear marc extract, were selected and their growth profiles and physiological functionalities were determined. Both of the selected yeasts established maximal growth by 20 hr of cultivation at 30℃ in pear marc extract. The cell-free extracts showed high antihypertensive angiotensin I-converting enzyme inhibitory activity of 68.9% and 52.1%, respectively. The extracts also displayed 9.2 U/mL and 12.0 U/mL of protease activity, respectively.

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“…Fibrinolytic activity was assayed by the method of Fayek and El-Sayed [24]. We added 0.5 mL of each sample to 3 mL of the substrate solution (0.6% fibrin in 0.1M McIlvaine buffer, pH 7.0) and incubated them at 40℃ for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Physiological Functionalities ofVitis hybrid(Sheridan)-Rubus coreanusRed Wine Made bySaccharomyces cerevisiae

et al. 2011

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Vitis hybrid (Sheridan)-Robus coreanus red wine was vinified by fermentation of a mixture of Vitis hybrid and Robus coreanus must at 25℃ for 10 days. The Vitis hybrid-Robus coreanus red wine had ethanol contents of 10.9%. It had high antihypertensive angiotensin I-converting enzyme (ACE) inhibitory activity of 57.8% and antioxidant activity of 64.8%. Changes in the physicochemical properties and functionality of the Vitis hybrid-Robus coreanus red wine was investigated during a post-fermentation period of three months. The ACE inhibitory activity of the red wine increased as the post-fermentation period prolonged, and showed the highest ACE inhibitory activity of 70.4% 60 days post-fermentation. However, the antioxidant activity declined significantly to 47.2% during the post-fermentation period of 60 days. In terms of sensory evaluation, the Vitis hybrid-Robus coreanus red wine had the best acceptability 60 days post-fermentation.

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Purification and properties of a fibrinolytic enzyme fromBacillus subtilis

Cited by 19 publications

References 9 publications

Extraction and Characteristics of Anti-obesity Lipase Inhibitor fromPhellinus linteus

Extraction and Characteristics of Anti-obesity Lipase Inhibitor fromPhellinus linteus

Growth Characteristics and Physiological Functionality of Yeasts in Pear Marc Extracts

Physiological Functionalities ofVitis hybrid(Sheridan)-Rubus coreanusRed Wine Made bySaccharomyces cerevisiae

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