Purification and properties of an extracellular protease of Aeromonas salmonicida, the causative agent of furunculosis

Shieh, H. S.; MacLean, Janet

doi:10.1016/0020-711x(75)90016-6

Cited by 49 publications

(27 citation statements)

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“…A variety of ECP activities of subsp, salmonicida have been described including glycerophospholipid-cholesterol acyltransferase (GCAT) (Buckley et al 1982, Lee & E k s 1990, a leucocytolysin (Fuller et al 1977), haemolysins (Titball & Munn 1981, Hastings & Ellis 1985, Fyfe et al 1987, Nomura e t al. 1988, Lee & Ellis 1990) and proteases (Shieh & McLean 1975, Mellergaard 1983, Tajima et al 1984, Hastings & Ellis 1985, Fyfe e t al. 1986, Fyfe et al 1987.…”

Section: "mentioning

confidence: 99%

Isolation of a new toxic protease from a strain of Aeromonas salmonicida subspecies achromogenes

Guðmundsdóttir¹,

Hastings²,

Ellis³

1990

Dis. Aquat. Org.

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Section: "mentioning

confidence: 99%

Isolation of a new toxic protease from a strain of Aeromonas salmonicida subspecies achromogenes

Guðmundsdóttir¹,

Hastings²,

Ellis³

1990

Dis. Aquat. Org.

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“…The cell envelope protein (7,15,36) and the extracellular products (2, 6, 21) of the bacterium have been investigated as the virulent factors causing the disease symptoms. The extracellular products include proteolytic (3, 25,27,28,30), glycerophospholipid-cholesterol acyltransferase (1,18), leukocytolytic (8), and hemolytic (14,22,33) activities. They reproduce the histopathological effects associated with furunculosis infection in rainbow trout.…”

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confidence: 99%

Purification and characterization of salmolysin, an extracellular hemolytic toxin from Aeromonas salmonicida

et al. 1988

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An extracellular hemolytic toxin of Aeromonas salmonicida, termed salmolysin, was purified 945-fold by ammonium sulfate precipitation, anion-exchange chromatography on DEAE-cellulose, and gel filtration chromatography on Sephadex G-100 and Sepharose 2B. Salmolysin appeared homogeneous upon cellulose acetate membrane electrophoresis and immunodiffusion analysis. The molecular weight of the toxin was estimated to be approximately 200,000 by the sedimentation equilibrium method. The UV absorption spectrum showed a maximum at 275 nm and a minimum at 262 nm. The isoelectric point was found to be at pl 5.4. Carbohydrate and protein analyses and other biochemical data indicated that salmolysin is a glycoprotein, containing approximately 62% carbohydrates. The toxin is a heat-labile substance and is stable at a neutral pH value. Ferrous ion inhibited the activity, whereas metal-chelating agents did not affect the activity. Sulfhydryl reagents did not inhibit the toxin, whereas reducing agents, such as L-cysteine and reduced glutathione, inhibited the toxin to a certain extent. Salmolysin was inactivated by a nonionic detergent but was stimulated by an anionic detergent, sodium deoxycholate, at a low concentration. The toxin was also inactivated by subtilisin and trypsin but was not inhibited by papain and pepsin. Salmolysin, with a remarkable hemolytic activity against salmonid erythrocytes, was lethal to rainbow trout when it was injected intramuscularly.

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“…Various A. salmonicida subspecies have been isolated from skin ulcerations in fish species such as salmonids ( Salmonidae ) (Shieh & Maclean, ; Wiklund & Dalsgaard, ), goldfish ( Carassius auratus ) (Elliott & Shotts, ), European flounder (Wiklund, ), turbot (Pedersen, Kofod, Dalsgaard, & Larsen, ; Toranzo & Barja, ), pike ( Esox sp.) (Wiklund, ) and common dab (Wiklund & Dalsgaard, ; Wiklund et al., ).…”

Section: Discussionmentioning

confidence: 99%

First isolation of Vibrio tapetis and an atypical strain of Aeromonas salmonicida from skin ulcerations in common dab (Limanda limanda) in the North Sea

Vercauteren

Swaef

Declercq

et al. 2017

Journal of Fish Diseases

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Skin ulcerations rank amongst the most prevalent lesions affecting wild common dab (Limanda limanda) with an increase in prevalence of up to 3.5% in the Belgian part of the North Sea. A complex aetiology of these ulcerations is suspected, and many questions remain on the exact factors contributing to these lesions. To construct the aetiological spectrum of skin ulcerations in flatfish, a one-day monitoring campaign was undertaken in the North Sea. Fifteen fish presented with one or more ulcerations on the pigmented and/or non-pigmented side. Pathological features revealed various stages of ulcerations with loss of epidermal and dermal tissue, inflammatory infiltrates and degeneration of the myofibers bordering the ulceration, albeit in varying degrees. Upon bacteriological examination, pure cultures of Vibrio tapetis were retrieved in high numbers from five fish and of Aeromonas salmonicida in one fish. The V. tapetis isolates showed cross-reactivity with the sera against the representative strain of serotype O2 originating form a carpet-shell clam (Ruditapes descussatus). Moreover, the A. salmonicida isolates displayed a previously undescribed vapA gene sequence (A-layer type) with possible specificity towards common dab. Further research is necessary to pinpoint the exact role of these agents in the development of skin ulcerations in common dab.

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Purification and properties of an extracellular protease of Aeromonas salmonicida, the causative agent of furunculosis

Cited by 49 publications

References 10 publications

Isolation of a new toxic protease from a strain of Aeromonas salmonicida subspecies achromogenes

Isolation of a new toxic protease from a strain of Aeromonas salmonicida subspecies achromogenes

Purification and characterization of salmolysin, an extracellular hemolytic toxin from Aeromonas salmonicida

First isolation of Vibrio tapetis and an atypical strain of Aeromonas salmonicida from skin ulcerations in common dab (Limanda limanda) in the North Sea

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