Purification and properties of extracellular glucosyltransferase from Streptococcus bovis

Abstract

Eight Streptococcus bovis strains were classified into 3 types on the basis of isoelectric point (pI) and molecular mass (M(r)) of extracellular glucosyltransferase. Strains ATCC 9809, 35034 and 43143 produced glucosyltransferase of pI 3.7 and M(r) 165 kDa; strains ATCC 15351, 27960 and 33317 produced glucosyltransferase of pI 4.1 and M(r) 140 kDa; strains ATCC 43085 and 43144 did not produce any glucosyltransferase. The glucosyltransferase form S. bovis 9809 was purified by Bio-Gel hydroxyapatite chromatography and DEAE-Toyopearl chromatography. The S. bovis 9809 glucosyltransferase was immunologically identical with the other 5 S. bovis glucosyltransferases and not related to mutants streptococcal glucosyltransferases. The specific activity, the optimum pH and the Km value for sucrose were 17.9 U/mg protein, 6.0 and 5.0 mM, respectively. The first 11 N-terminal amino acid residues of the glucosyltransferases were DETSAVTLTRE, and the region was hydrophilic. The glucosyltransferases from S. bovis 9809 and 3317 synthesized from sucrose 1, 6-alpha-D-glucan with 9 and 2 mol%, 1, 3, 6-alpha-branched glucose, respectively.