Purification and Properties of Hibiscus Chlorotic Ringspot Virus

Waterworth, H. E.; Lawson, R. H.; Monroe, R. L.

doi:10.1094/phyto-66-570

Cited by 29 publications

(23 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Virions were isolated from leaves of inoculated squash as described for Hibiscus chlorotic ringspot virus by Waterworth and colleagues (50) and analyzed by electron microscopy on 300-mesh formvar-coated copper grids after staining with 5% (wt/vol) uranyl acetate. Measurements of 100 particles representative of SqVYV were used to determine a mean particle length.…”

Section: E-xtra *mentioning

confidence: 99%

Identification and Characterization of a Novel Whitefly-Transmitted Member of the Family Potyviridae Isolated from Cucurbits in Florida

Adkins¹,

Webb²,

Achor³

et al. 2007

Phytopathology®

View full text Add to dashboard Cite

A novel whitefly-transmitted member of the family Potyviridae was isolated from a squash plant (Cucurbita pepo) with vein yellowing symptoms in Florida. The virus, for which the name Squash vein yellowing virus (SqVYV) is proposed, has flexuous rod-shaped particles of approximately 840 nm in length. The experimental host range was limited to species in the family Cucurbitaceae, with the most dramatic symptoms observed in squash and watermelon, but excluded all tested species in the families Amaranthaceae, Apocynaceae, Asteraceae, Chenopodiaceae, Fabaceae, Malvaceae, and Solanaceae. The virus was transmitted by whiteflies (Bemisia tabaci) but was not transmitted by aphids (Myzus persicae). Infection by SqVYV induced inclusion bodies visible by electron and light microscopy that were characteristic of members of the family Potyviridae. Comparison of the SqVYV coat protein gene and protein sequences with those of recognized members of the family Potyviridae indicate that it is a novel member of the genus Ipomovirus. A limited survey revealed that SqVYV also was present in watermelon plants suffering from a vine decline and fruit rot recently observed in Florida and was sufficient to induce these symptoms in greenhouse-grown watermelon, suggesting that SqVYV is the likely cause of this disease.

show abstract

Section: E-xtra *mentioning

confidence: 99%

Identification and Characterization of a Novel Whitefly-Transmitted Member of the Family Potyviridae Isolated from Cucurbits in Florida

Adkins¹,

Webb²,

Achor³

et al. 2007

Phytopathology®

View full text Add to dashboard Cite

show abstract

“…HCRSV has been identified in hibiscus species worldwide, including the United States and south-east Asia (Waterworth et al, 1976;Wong and Chng, 1992). In the US it infects kenaf (Hibiscus cannabinus), a tree crop of major interest to the wood pulp industry (Johnson, 2001).…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional reconstruction of hibiscus chlorotic ringspot virus

Doan

Lee

Laurinmäki

et al. 2003

Journal of Structural Biology

View full text Add to dashboard Cite

“…The virus is found worldwide where hibiscus is cultivated (16,41,42). The symptoms on HCRSV-infected plants range from generalized mottle to chlorotic ringspots and vein-banding patterns, severe stunting, and flower distortion (42,45). HCRSV possesses a singlestranded positive-sense RNA that is not polyadenylated at the 3Ј terminus.…”

mentioning

confidence: 99%

A Six-Nucleotide Segment within the 3′ Untranslated Region of Hibiscus Chlorotic Ringspot Virus Plays an Essential Role in Translational Enhancement

Koh

Liu

Wong

2002

J Virol

View full text Add to dashboard Cite

RNA plant viruses use various translational regulatory mechanisms to control their gene expression.Translational enhancement of viral mRNAs that leads to higher levels of protein synthesis from specific genes may be essential for the virus to successfully compete for cellular translational machinery. The control elements have yet to be analyzed for members of the genus Carmovirus, a small group of plant viruses with positive-sense RNA genomes. In this study, we examined the 3 untranslated region (UTR) of hibiscus chlorotic ringspot virus (HCRSV) genomic RNA (gRNA) and subgenomic RNA (sgRNA) for its role in the translational regulation of viral gene expression. The results showed that the 3 UTR of HCRSV significantly enhanced the translation of several open reading frames on gRNA and sgRNA and a viral gene in a bicistronic construct with an inserted internal ribosome entry site. Through deletion and mutagenesis studies of both the bicistronic construct and full-length gRNA, we demonstrated that a six-nucleotide sequence, GGGCAG, that is complementary to the 3 region of the 18S rRNA and a minimal length of 180 nucleotides are required for the enhancement of translation induced by the 3 UTR.The 3Ј untranslated regions (UTRs) of plant virus RNAs are likely to function in a manner similar to that of the corresponding regions of cellular mRNA in regulating translation and maintaining RNA stability. Due to their great variety in terminal structures, the 3Ј UTRs of plus-strand viral RNAs play varied roles in replication and translation (5). For example, the turnip yellow mosaic virus RNA contains a 3Ј tRNA mimic believed to regulate minus-strand RNA synthesis (6). For brome mosaic virus, the 3Ј UTR harbors a unique promoter element that directs minus-strand RNA synthesis (23). The 3Ј UTR of tobacco mosaic virus contains a translation enhancer (8).Hibiscus chlorotic ringspot virus (HCRSV), a member of the genus Carmovirus, is an isometric monopartite plant virus which measures 28 nm in diameter. The virus is found worldwide where hibiscus is cultivated (16,41,42). The symptoms on HCRSV-infected plants range from generalized mottle to chlorotic ringspots and vein-banding patterns, severe stunting, and flower distortion (42,45). HCRSV possesses a singlestranded positive-sense RNA that is not polyadenylated at the 3Ј terminus. The genomic RNA (gRNA) is 3,911 nucleotides (nt) long and has the potential to encode seven open reading frames (ORFs), including two novel ORFs, p23 and p25 (12). Two 3Ј-coterminated subgenomic RNA (sgRNA) species have been identified.In this study, we examined the 3Ј UTR of HCRSV gRNA and sgRNA for its role in the regulation of translation. Through deletion studies and elimination of putative translation enhancer elements, we were able to identify the prerequisites required for the translational enhancement induced by the 3Ј UTR of HCRSV. The results showed that the 3Ј UTR of HCRSV differentially enhanced the translation of ORFs on gRNA and sgRNA and an HCRSV gene in a bicistronic construct by 1.5-t...

show abstract

Purification and Properties of Hibiscus Chlorotic Ringspot Virus

Cited by 29 publications

References 0 publications

Identification and Characterization of a Novel Whitefly-Transmitted Member of the Family Potyviridae Isolated from Cucurbits in Florida

Identification and Characterization of a Novel Whitefly-Transmitted Member of the Family Potyviridae Isolated from Cucurbits in Florida

Three-dimensional reconstruction of hibiscus chlorotic ringspot virus

A Six-Nucleotide Segment within the 3′ Untranslated Region of Hibiscus Chlorotic Ringspot Virus Plays an Essential Role in Translational Enhancement

Contact Info

Product

Resources

About