Purification and properties of histidine decarboxylase from Lactobacillus 30a.

Rosenthaler, J.; Guirard, Beverly M.; Chang, Gu‐Gang; Snell, Esmond E.

doi:10.1073/pnas.54.1.152

Cited by 58 publications

(25 citation statements)

References 12 publications

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“…Prior to performing in vitro studies, we characterized our cultured mast cell line by measuring typical markers of mast cells by real-time PCR, including: HDC, MAO-B (enzyme responsible for the breakdown of histamine 43 ), chymase, tryptase, and c-kit. We also evaluated the expression of CK-19 (the cholangiocyte-specific marker 25,28 ) by real-time PCR.…”

Section: Treatment With Cromolyn Sodium In Vitro Decreases Mast Cell mentioning

confidence: 99%

Inhibition of mast cell-derived histamine secretion by cromolyn sodium treatment decreases biliary hyperplasia in cholestatic rodents

Kennedy

Hargrove

Graf

et al. 2014

Laboratory Investigation

143

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Cholangiopathies are characterized by dysregulation of the balance between biliary growth and loss. We have shown that histamine (HA) stimulates biliary growth via autocrine mechanisms. To evaluate the paracrine effects of mast cell (MC) stabilization on biliary proliferation, sham or BDL rats were treated by IP-implanted osmotic pumps filled with saline or cromolyn sodium (24 mg/kg BW/day (inhibits MC histamine release)) for 1 week. Serum, liver blocks and cholangiocytes were collected. Histidine decarboxylase (HDC) expression was measured using real-time PCR in cholangiocytes. Intrahepatic bile duct mass (IBDM) was evaluated by IHC for CK-19. MC number was determined using toluidine blue staining and correlated to IBDM. Proliferation was evaluated by PCNA expression in liver sections and purified cholangiocytes. We assessed apoptosis using real-time PCR and IHC for BAX. Expression of MC stem factor receptor, c-kit, and the proteases chymase and tryptase were measured by real-time PCR. HA levels were measured in serum by EIA. In vitro, MCs and cholangiocytes were treated with 0.1% BSA (basal) or cromolyn (25 mM) for up to 48 h prior to assessing HDC expression, HA levels and chymase and tryptase expression. Supernatants from MCs treated with or without cromolyn were added to cholangiocytes before measuring (i) proliferation by MTT assays, (ii) HDC gene expression by real-time PCR and (iii) HA release by EIA. In vivo, cromolyn treatment decreased BDL-induced: (i) IBDM, MC number, and biliary proliferation; (ii) HDC and MC marker expression; and (iii) HA levels. Cromolyn treatment increased cholangiocyte apoptosis. In vitro, cromolyn decreased HA release and chymase and tryptase expression in MCs but not in cholangiocytes. Cromolyn-treated MC supernatants decreased biliary proliferation and HA release. These studies provide evidence that MC histamine is key to biliary proliferation and may be a therapeutic target for the treatment of cholangiopathies.

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Section: Treatment With Cromolyn Sodium In Vitro Decreases Mast Cell mentioning

confidence: 99%

Inhibition of mast cell-derived histamine secretion by cromolyn sodium treatment decreases biliary hyperplasia in cholestatic rodents

Kennedy

Hargrove

Graf

et al. 2014

Laboratory Investigation

143

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“…It is the protein moiety of a given B, enzyme that determines which of the many potential pathways is adopted by the coenzyme-substrate adduct. PLP and pyridoxamine-5'-phosphate serve also as cofactors in a particular group of bacterial enzymes that participate in the biosynthesis of dideoxy and deoxyamine sugars (Rubinstein and Strominger, 1974) and are evolutionarily related with the B, enzymes in amino acid metabolism. The role of PLP in glucan phosphorylases (Palm et al, 1990) is entirely different; these enzymes constitute another independent evolutionary lineage that will not be discussed here in detail.…”

Section: Introductionmentioning

confidence: 99%

The Molecular Evolution of Pyridoxal‐5′‐Phosphate‐Dependent Enzymes

Mehta

Christen

2000

Advances in Enzymology - And Related Areas of Molecular Biology

140

159

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“…However, the decarboxylation of N p -, and N t -methyl-L-histidines occurs very slowly under the experimental conditions studied. 21 In our experimental conditions we observed that HDC converts N p -methyl-L-histidine into pMeHA more effectively than in the case of N t -methyl-Lhistidine. Over a period of one week the decarboxylation of N p -methyl-L-histidine the amine is formed with nearly 80% chemical yield.…”

Section: Resultsmentioning

confidence: 50%