Purification and properties of l-valyl-sRNA synthetase from Escherichia coli

Aminoacyl-tRNA synthetases from yeast: generality of chemical proofreading in the prevention of misaminoacylation of tRNA

“…The specificity of valyl-tRNA synthetase (E. coli) has previously been examined by a number of workers (Bergmann et al, 1961;Loftfield & Eigner, 1966;George & Meister, 1967;Yaniv & Gros, 1969;Owens & Bell, 1970). The results…”

“… found only trace activity with isoleucine whileGeorge & Meister (1967) observed a negligible extent of ATP-PPj exchange with isoleucine. Alanine, which was inactive in the system of George & Meister (1967), inhibited EXXX.…”

“…a References are as follows: (1)Loftfield & Eigner (1966); (2)George & Meister (1967); (3)Freundlich (1967); (4)Igloi et al (1977); (5) Bergmann et al (1961); (6) Owens & Bell (1970); (7) Anderson & Fowden (1970b); (8) Chuang et al (1967); (9) Yaniv & Gros (1969); (10) Kondo & Woese (1969); (11) Berg et al (1961); (12) Arca et al (1967); (13) Smulson & Rabinovitz (1968); (14) Holler et al (1973); (15) Trupin et al (1966); (16) Old & Jones (1977); (17) Stern & Mehler (1965); (18) Harding & DeShazo (1967); (19) Nevinsky et al (1974); (20) Lemaire et al (1967); (21) Calendar & Berg (1966); (22)Mitra & Mehler (1967); (23)Peterson & Fowden (1965); (24)Papas & Mehler (1970); (25)Fowden & Richmond (1963); (26) Atherly & Bell (1964); (27) Conway et al (1962); (28) Anderson & Fowden (1970a); (29) Santi & Danenberg (1971); (30) Smith & Fowden (1968); (31) this work; (32) Porter et al (1977); (33) Lodeman et al (1977); (34) Shirota…”

Hydrolytic action of aminoacyl-tRNA synthetases from baker's yeast. "Chemical proofreading" of Thr-tRNAVal by valyl-tRNA synthetase studied with modified tRNAVal and amino acid analogs

“…As is the case for normal Sn2 reactions, if the center of nucleophilic attack is optically active, inversion of the configuration takes place. In the case of threonine, formation of a /3-lactone from the natural L-threonine would, after hydrolysis, give the inverted L-allothreonine which is not a substrate for valyl-tRNA synthetase (in the PP exchange, at least;Bergmann et al, 1961;George and Meister, 1967). During AMP/PP independent hydrolysis under aminoacylating conditions when the ATP concentration is not limiting, such a conversion of threonine to allothreonine should lead to a plateau in the amount of AMP produced at a level equal to the initial threonine concentration.…”

Addendum

“…Information on the purification of various aminoacyl-s-RNA synthetases from Escherichia coli has been collected together as a series of papers (Cantoni & Davies, 1966). In addition, further reports have appeared on the purification of these enzymes specific for cysteine (Bucovaz, Morrison Sr Wood, 1966); separate enzymes for glutamic acid and glutamine (Deutscher, Lemaire, Dorizzi & Labouesse, 1967); phenylalanine (Stulberg, 1967) and valine (Lagerkvist, Rymo & Waldenstrom, 1966;George & Meister, 1967).…”