Purification and properties of tyrosinases from Vibrio tyrosinaticus

Pomerantz, Seymour H.; Murthy, Vadiraja V.

doi:10.1016/s0003-9861(74)80010-x

Cited by 89 publications

(41 citation statements)

References 35 publications

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“…This point is important, since laccase activity has been involved in the melanization of A. lipoferum (17) and in the formation of fungal dihydroxynaphthalene-melanins (15). In contrast, our data support most of the bacterial strains so far studied, where L-tyrosine is the most common substrate for melanization and tyrosinase has been linked to this process (27,32).…”

Section: Discussionsupporting

confidence: 85%

Marinomonas mediterranea MMB-1 Transposon Mutagenesis: Isolation of a Multipotent Polyphenol Oxidase Mutant

Solano¹,

Lucas-Elı́o

Fernández³

et al. 2000

J Bacteriol

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Marinomonas mediterranea is a melanogenic marine bacterium expressing a multifunctional polyphenol oxidase (PPO) able to oxidize substrates characteristic for laccases and tyrosinases, as well as produce a classical tyrosinase. A new and quick method has been developed for screening laccase activity in culture plates to detect mutants differentially affected in this PPO activity. Transposon mutagenesis has been applied for the first time to M. mediterranea by using different minitransposons loaded in R6K-based suicide delivery vectors mobilizable by conjugation. Higher frequencies of insertions were obtained by using mini-Tn10 derivatives encoding kanamycin or gentamycin resistance. After applying this protocol, a multifunctional PPO-negative mutant was obtained. By using the antibiotic resistance cassette as a marker, flanking regions were cloned. Then the wild-type gene was amplified by PCR and was cloned and sequenced. This is the first report on cloning and sequencing of a gene encoding a prokaryotic enzyme with laccase activity. The deduced amino acid sequence shows the characteristic copper-binding sites of other blue copper proteins, including fungal laccases. In addition, it shows some extra copper-binding sites that might be related to its multipotent enzymatic capability.

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Section: Discussionsupporting

confidence: 85%

Marinomonas mediterranea MMB-1 Transposon Mutagenesis: Isolation of a Multipotent Polyphenol Oxidase Mutant

Solano¹,

Lucas-Elı́o

Fernández³

et al. 2000

J Bacteriol

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“…This brownish to black pigment is synthesized by the enzyme tyrosinase (monophenol, L-dopa: oxygen oxidoreductase; EC 1.14.18.1), a copper-containing monooxygenase that has both cresolase and catecholase activities (23). These enzymatic activities mediate the orthohydroxylation of tyrosine and other monophenols and aromatic amines to o-diphenols and the subsequent oxidation of o-diphenols to o-quinones; these steps are followed by several nonenzymatic oxidation and polymerization steps (25). Several important functions and properties have been associated with these pigments (2).…”

mentioning

confidence: 99%

Melanin production by Rhizobium meliloti GR4 is linked to nonsymbiotic plasmid pRmeGR4b: cloning, sequencing, and expression of the tyrosinase gene mepA

et al. 1993

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Melanin production by Rhizobium meliloti GR4 is linked to nonsymbiotic plasmid pRmeGR4b (140 MDa). Transfer of this plasmid to GR4-cured derivatives or to Agrobacterium tumefaciens enables these bacteria to produce melanin. Sequence analysis of a 3.5-kb PstI fragment of plasmid pRmeGR4b has revealed the presence of a open reading frame 1,481-bp that codes for a protein whose sequence shows strong homology to two conserved regions involved in copper binding in tyrosinases and hemocyanins. In vitro-coupled transcription-translation experiments showed that this open reading frame codes for a 55-kDa polypeptide. Melanin production in GR4 is not under the control of the RpoN-NifA regulatory system, unlike that in R. leguminosarum bv. phaseoli 8002. The GR4 tyrosinase gene could be expressed in Escherichia coli under the control of the lacZ promoter. For avoiding confusion with mel genes (for melibiose), a change of the name of the previously reported mel genes of R. leguminosarum bv. phaseoli and other organisms to mep genes (for melanin production) is proposed.

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“…Phacomelanin is produced by some vibrio strains (Pomerantz and murthy, 1974;Ivins and Holmes, 1981) Some S. marcescens strains are able to produce different pigments. The best known prodigiosin is a linear tripyrrole attach to the cytoplasmic membrane (Vinas et al, 1983;Parachuri and Harshey, 1987).…”

Section: Role Of Tyrosine In Pigmentationmentioning

confidence: 99%

Effect of Certain Compounds on the Pigmentation Characteristis of Some Bacterial Isolates

Qureshi¹,

Sankaran²,

Subbaiah³

2018

Int.J.Curr.Microbiol.App.Sci

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Purification and properties of tyrosinases from Vibrio tyrosinaticus

Cited by 89 publications

References 35 publications

Marinomonas mediterranea MMB-1 Transposon Mutagenesis: Isolation of a Multipotent Polyphenol Oxidase Mutant

Marinomonas mediterranea MMB-1 Transposon Mutagenesis: Isolation of a Multipotent Polyphenol Oxidase Mutant

Melanin production by Rhizobium meliloti GR4 is linked to nonsymbiotic plasmid pRmeGR4b: cloning, sequencing, and expression of the tyrosinase gene mepA

Effect of Certain Compounds on the Pigmentation Characteristis of Some Bacterial Isolates

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